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On the Physicochemical Control of Co...

Bradley, Patrick Anthony.

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On the Physicochemical Control of Collagen Fibrillogenesis and Biomineralization.

Record Type:	Electronic resources : Monograph/item
Title/Author:	On the Physicochemical Control of Collagen Fibrillogenesis and Biomineralization./
Author:	Bradley, Patrick Anthony.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2019,
Description:	231 p.
Notes:	Source: Dissertations Abstracts International, Volume: 80-11, Section: B.
Contained By:	Dissertations Abstracts International80-11B.
Subject:	Bioengineering. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=13861417
ISBN:	9781392151020

On the Physicochemical Control of Collagen Fibrillogenesis and Biomineralization.
Bradley, Patrick Anthony.

On the Physicochemical Control of Collagen Fibrillogenesis and Biomineralization. - Ann Arbor : ProQuest Dissertations & Theses, 2019 - 231 p.

Source: Dissertations Abstracts International, Volume: 80-11, Section: B.

Thesis (Ph.D.)--Northeastern University, 2019.

This item must not be sold to any third party vendors.

Tissue engineered collagen-based scaffolds have been widely explored for their ability to provide a three-dimensional, variable-stiffness extracellular matrix mimic capable of directing local cellular morphology, differentiation, and gene expression. Of the numerous approaches to fabricate collagen scaffolds, the method that most closely emulates the density and architecture of native tissue is the liquid crystal manipulation method. Despite significant advances in the last 25 years, the development of collagen scaffolds from liquid crystal phase solutions still faces two major hurdles: 1) an inability to control orientation of fibril arrays over clinically-relevant distances; 2) an inability to recapitulate the native-state morphology of collagen fibrils in connective tissues.The goal of this dissertation was to overcome the limitations of collagen self-assembly in the crowded state through the tuning of relevant physicochemical assembly parameters. To this end, we outline the design, optimization, and implementation of a liquid crystal manipulation method to fabricate collagen scaffolds with long-range crimp-like organizational order. To induce native-state fibril morphological characteristics, the assembly process was modified to include the application of ultrasound to densifying collagen solutions. This led to the development of novel collagen materials that exhibit native-like fibril diameters, characteristic D-periodic banding structures, and robust anisotropic mechanical properties representative of some dense connective tissues. In the next investigation, we demonstrated that material stability, including mechanical properties, solubility, and thermal resistance, could be tuned by varying the duration of applied ultrasound. Lastly, we investigated the effect of mechanical loading in the early stages of biomineralization on mineral and mechanical outcomes in an ex vivo tendon model.Our traditional understanding of supramolecular systems relies on understanding the relationship between molecular structure and function. By contrast, the driving hypothesis behind the work detailed in this dissertation is that function is inexorably linked to the system's supramolecular energy landscape. By altering this landscape through physicochemical means, we herein demonstrate control of molecular and supramolecular orientation, assembly, and stability.

ISBN: 9781392151020Subjects--Topical Terms:

657580
Bioengineering.

On the Physicochemical Control of Collagen Fibrillogenesis and Biomineralization.
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520 $a Tissue engineered collagen-based scaffolds have been widely explored for their ability to provide a three-dimensional, variable-stiffness extracellular matrix mimic capable of directing local cellular morphology, differentiation, and gene expression. Of the numerous approaches to fabricate collagen scaffolds, the method that most closely emulates the density and architecture of native tissue is the liquid crystal manipulation method. Despite significant advances in the last 25 years, the development of collagen scaffolds from liquid crystal phase solutions still faces two major hurdles: 1) an inability to control orientation of fibril arrays over clinically-relevant distances; 2) an inability to recapitulate the native-state morphology of collagen fibrils in connective tissues.The goal of this dissertation was to overcome the limitations of collagen self-assembly in the crowded state through the tuning of relevant physicochemical assembly parameters. To this end, we outline the design, optimization, and implementation of a liquid crystal manipulation method to fabricate collagen scaffolds with long-range crimp-like organizational order. To induce native-state fibril morphological characteristics, the assembly process was modified to include the application of ultrasound to densifying collagen solutions. This led to the development of novel collagen materials that exhibit native-like fibril diameters, characteristic D-periodic banding structures, and robust anisotropic mechanical properties representative of some dense connective tissues. In the next investigation, we demonstrated that material stability, including mechanical properties, solubility, and thermal resistance, could be tuned by varying the duration of applied ultrasound. Lastly, we investigated the effect of mechanical loading in the early stages of biomineralization on mineral and mechanical outcomes in an ex vivo tendon model.Our traditional understanding of supramolecular systems relies on understanding the relationship between molecular structure and function. By contrast, the driving hypothesis behind the work detailed in this dissertation is that function is inexorably linked to the system's supramolecular energy landscape. By altering this landscape through physicochemical means, we herein demonstrate control of molecular and supramolecular orientation, assembly, and stability.
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