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Development of a 3D Collagen-Hydroxy...

Golz, Brian T.

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Development of a 3D Collagen-Hydroxyapatite Composite in vitro Culture Model for Osteoblasts and Osteocytes.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Development of a 3D Collagen-Hydroxyapatite Composite in vitro Culture Model for Osteoblasts and Osteocytes./
作者:	Golz, Brian T.
出版者:	Ann Arbor : ProQuest Dissertations & Theses, : 2017,
面頁冊數:	54 p.
附註:	Source: Masters Abstracts International, Volume: 79-09.
Contained By:	Masters Abstracts International79-09.
標題:	Cellular biology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10638525
ISBN:	9780355617115

Development of a 3D Collagen-Hydroxyapatite Composite in vitro Culture Model for Osteoblasts and Osteocytes.
Golz, Brian T.

Development of a 3D Collagen-Hydroxyapatite Composite in vitro Culture Model for Osteoblasts and Osteocytes. - Ann Arbor : ProQuest Dissertations & Theses, 2017 - 54 p.

Source: Masters Abstracts International, Volume: 79-09.

Thesis (M.S.B.M.E.)--Purdue University, 2017.

This item must not be sold to any third party vendors.

Osteoporosis is a disease characterized by a severe loss in bone mineral density, puttingindividuals at elevated risk of bone fracture. The disorder is extremely prevalent, with highassociated costs. Pharmaceutical treatments exist, but currently have severe drawbacks,and preventative measures are eﬀective primarily within an individual's ﬁrst two decadesof life, long before the normal age of diagnosis. To better develop treatments in a highthroughput physiological setting, an in vitro model for bone cell culture that accuratelyrecapitulates the in vivo cellular environment is needed. Thus, a 3D culture system has beendeveloped utilizing porcine skin collagen oligomers (PSC) and precipitated hydroxyapatite(HA) nanoparticles to support de novo bone formation and diﬀerentiation of osteoblastsinto osteocytes. Cells isolated from DMP1-Cre x mT/mG mice were cultured in 3D matriceswith diﬀering concentrations of PSC and HA for 3 and 56 days in osteogenic medium, beforebeing sampled for testing. Fixed culture sections were imaged using confocal microscopyto quantify diﬀerentiation of osteoblasts to osteocytes, indicated by the shift from cellularexpression of red ﬂuorescent protein to green ﬂuorescent protein through the activation ofthe promoter for the osteocyte-speciﬁc dentin matrix protein 1. These data demonstrateda signiﬁcant proportional increase in late osteoblasts and and osteocytes. µCT analysisshowed a signiﬁcant increase in mineralization of the ECM, exclusive to cultures initiallyformed with HA. This was reﬂected by increased mechanical stiﬀness at high strain in someculture conditions.

ISBN: 9780355617115Subjects--Topical Terms:

3172791
Cellular biology.

Development of a 3D Collagen-Hydroxyapatite Composite in vitro Culture Model for Osteoblasts and Osteocytes.
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