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Roles of Phospholipases and Ribosoma...

Su, Yuan.

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Roles of Phospholipases and Ribosomal S6 Kinase in Lipid Remodeling and Growth in Arabidopsis Response to Phosphate Deprivation.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Roles of Phospholipases and Ribosomal S6 Kinase in Lipid Remodeling and Growth in Arabidopsis Response to Phosphate Deprivation./
Author:	Su, Yuan.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2018,
Description:	157 p.
Notes:	Source: Dissertation Abstracts International, Volume: 79-10(E), Section: B.
Contained By:	Dissertation Abstracts International79-10B(E).
Subject:	Molecular biology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10813763
ISBN:	9780438001404

Roles of Phospholipases and Ribosomal S6 Kinase in Lipid Remodeling and Growth in Arabidopsis Response to Phosphate Deprivation.
Su, Yuan.

Roles of Phospholipases and Ribosomal S6 Kinase in Lipid Remodeling and Growth in Arabidopsis Response to Phosphate Deprivation. - Ann Arbor : ProQuest Dissertations & Theses, 2018 - 157 p.

Source: Dissertation Abstracts International, Volume: 79-10(E), Section: B.

Thesis (Ph.D.)--University of Missouri - Saint Louis, 2018.

Phosphate (Pi) is one of three macronutrients for plants, which is vital for plant growth and development. Understanding the mechanism by which plants respond and adapt to Pi deficiency not only unveils functions of genes and pathways involved, but also provides potential tools to manipulate crops to better stand Pi stress in low Pi-containing lands. One of the significant metabolic changes in plants under Pi starvation is the membrane lipid remodeling that converts Pi-containing lipids such as phospholipids to Pi-free lipids, such as glycolipids. To elucidate the metabolism and regulation of lipid remodeling, this dissertation characterizes the role of two phospholipases, phospholipase D zeta2 (PLD?2) and nonspecific phospholipase C4 (NPC4) in the lipid remodeling process. I generated double knockout pldzeta2npc4 Arabidopsis and performed comprehensive growth and membrane lipid analysis in single and double knockout under Pi deprivation. NPC4 contributed DGDG accumulation at an early stage of Pi deprivation in roots while PLDzeta2 displayed a dominant effect on lipid remodeling at a later stage of Pi deprivation in leaves. In addition, NPC4 facilitated root hair elongation, but had no effect on root hair density. By comparison, PLDzeta2 constrained both root hair elongation and density. The results indicate that PLDzeta2 and NPC4 mediate the Pi deprivation-induced lipid remodeling in a tissue- and time-specific manner, and that PLDzeta2 negatively modulates root hair density and length whereas NPC4 promotes root hair elongation in response to Pi deprivation. To probe the regulation of membrane lipid remodeling, I found that S6K, a ribosomal protein kinase in the TOR (Target of Rapamycin) signaling pathway, binds to phosphatidic acid (PA), a central lipid intermediate. Knockout of S6K2 but not S6K1 in Arabidopsis impeded membrane lipid remodeling and root growth in response to Pi deficiency. In addition, comparative lipidomic profiling and growth analysis of s6k2, phr1(PHOSPHATE RESPONSE 1) and s6k2phr1 indicate that S6K2 participates in the same pathway as PHR1 because similar growth phenotypes and membrane lipid composition were observed between single mutants and double mutants. The results indicate that S6K2 is part of the regulatory pathway that controls lipid remodeling and growth adaption to Pi limitation.

ISBN: 9780438001404Subjects--Topical Terms:

517296
Molecular biology.

Roles of Phospholipases and Ribosomal S6 Kinase in Lipid Remodeling and Growth in Arabidopsis Response to Phosphate Deprivation.
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520 $a Phosphate (Pi) is one of three macronutrients for plants, which is vital for plant growth and development. Understanding the mechanism by which plants respond and adapt to Pi deficiency not only unveils functions of genes and pathways involved, but also provides potential tools to manipulate crops to better stand Pi stress in low Pi-containing lands. One of the significant metabolic changes in plants under Pi starvation is the membrane lipid remodeling that converts Pi-containing lipids such as phospholipids to Pi-free lipids, such as glycolipids. To elucidate the metabolism and regulation of lipid remodeling, this dissertation characterizes the role of two phospholipases, phospholipase D zeta2 (PLD?2) and nonspecific phospholipase C4 (NPC4) in the lipid remodeling process. I generated double knockout pldzeta2npc4 Arabidopsis and performed comprehensive growth and membrane lipid analysis in single and double knockout under Pi deprivation. NPC4 contributed DGDG accumulation at an early stage of Pi deprivation in roots while PLDzeta2 displayed a dominant effect on lipid remodeling at a later stage of Pi deprivation in leaves. In addition, NPC4 facilitated root hair elongation, but had no effect on root hair density. By comparison, PLDzeta2 constrained both root hair elongation and density. The results indicate that PLDzeta2 and NPC4 mediate the Pi deprivation-induced lipid remodeling in a tissue- and time-specific manner, and that PLDzeta2 negatively modulates root hair density and length whereas NPC4 promotes root hair elongation in response to Pi deprivation. To probe the regulation of membrane lipid remodeling, I found that S6K, a ribosomal protein kinase in the TOR (Target of Rapamycin) signaling pathway, binds to phosphatidic acid (PA), a central lipid intermediate. Knockout of S6K2 but not S6K1 in Arabidopsis impeded membrane lipid remodeling and root growth in response to Pi deficiency. In addition, comparative lipidomic profiling and growth analysis of s6k2, phr1(PHOSPHATE RESPONSE 1) and s6k2phr1 indicate that S6K2 participates in the same pathway as PHR1 because similar growth phenotypes and membrane lipid composition were observed between single mutants and double mutants. The results indicate that S6K2 is part of the regulatory pathway that controls lipid remodeling and growth adaption to Pi limitation.
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