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A Cross-Sectional Study Comparing To...

Berkhouse, Hudson Taylor.

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A Cross-Sectional Study Comparing Torque Teno Virus Infection and Bushmeat Exposure among Pneumonia Patients: Sarawak, Malaysia.

Record Type:	Electronic resources : Monograph/item
Title/Author:	A Cross-Sectional Study Comparing Torque Teno Virus Infection and Bushmeat Exposure among Pneumonia Patients: Sarawak, Malaysia./
Author:	Berkhouse, Hudson Taylor.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2017,
Description:	53 p.
Notes:	Source: Masters Abstracts International, Volume: 57-02.
Contained By:	Masters Abstracts International57-02(E).
Subject:	Epidemiology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10640873
ISBN:	9780355579048

A Cross-Sectional Study Comparing Torque Teno Virus Infection and Bushmeat Exposure among Pneumonia Patients: Sarawak, Malaysia.
Berkhouse, Hudson Taylor.

A Cross-Sectional Study Comparing Torque Teno Virus Infection and Bushmeat Exposure among Pneumonia Patients: Sarawak, Malaysia. - Ann Arbor : ProQuest Dissertations & Theses, 2017 - 53 p.

Source: Masters Abstracts International, Volume: 57-02.

Thesis (M.S.)--Duke University, 2017.

Background: Torque Teno Virus (TTV) is ubiquitous, possibly zoonotic, and has potential for clinical and global health research application. The goals of the current study were to determine prevalence of TTV among pneumonia patients in two Malaysian hospitals, compare results from qPCR and conventional PCR detection methods, and to compare TTV infection against self-reported exposure to bushmeat products.

ISBN: 9780355579048Subjects--Topical Terms:

568544
Epidemiology.

A Cross-Sectional Study Comparing Torque Teno Virus Infection and Bushmeat Exposure among Pneumonia Patients: Sarawak, Malaysia.
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100 1 $a Berkhouse, Hudson Taylor. $3 3429316
245 1 2 $a A Cross-Sectional Study Comparing Torque Teno Virus Infection and Bushmeat Exposure among Pneumonia Patients: Sarawak, Malaysia.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2017
300 $a 53 p.
500 $a Source: Masters Abstracts International, Volume: 57-02.
500 $a Adviser: Greg Gray.
502 $a Thesis (M.S.)--Duke University, 2017.
520 $a Background: Torque Teno Virus (TTV) is ubiquitous, possibly zoonotic, and has potential for clinical and global health research application. The goals of the current study were to determine prevalence of TTV among pneumonia patients in two Malaysian hospitals, compare results from qPCR and conventional PCR detection methods, and to compare TTV infection against self-reported exposure to bushmeat products.
520 $a Methods: Medical officers obtained plasma, PBMC, and NP swab samples, along with bushmeat exposure information from 34 pneumonia patients in Sibu and Kapit hospitals. Samples were tested for TTV using qPCR. Results were corroborated using conventional PCR. Cornfield's and McNemar's exact methods were used to analyze infection by exposure, and agreement between PCR results respectively. Diagnostic abilities of the qPCR test were analyzed using conventional PCR as the gold standard.
520 $a Results: Conventional PCR reported 17.65% TTV prevalence, while qPCR reported 91.18%. Household size (OR = 0.00, 95% CI = 0.00, 0.96) and gender (OR = infinity, 95% CI = 1.39, infinity) had statistically significant impacts on odds of infection. The qPCR method showed 100% sensitivity, but 7.69% specificity.
520 $a Conclusions: Results suggest low prevalence of TTV within the study population, and reaffirm findings by other researchers that smaller household size and being female are associated with decreased odds of TTV infection. Though statistically insignificant, indications that exposure to bushmeat in general increases odds of TTV infection should be investigated further. Researchers must be aware of differences in diagnostic capabilities between PCR methods for TTV detection when designing their own diagnostic studies or conducting TTV related literature reviews.
590 $a School code: 0066.
650 4 $a Epidemiology. $3 568544
650 4 $a Virology. $3 642304
650 4 $a Animal diseases. $3 3181862
650 4 $a Southeast Asian studies. $3 3344898
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710 2 $a Duke University. $b Global Health. $3 2092989
773 0 $t Masters Abstracts International $g 57-02(E).
790 $a 0066
791 $a M.S.
792 $a 2017
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10640873