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Molecular Characterization of the Lo...

Ibba, Maria Itria.

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Molecular Characterization of the Low Molecular Weight Glutenin Subunit Gene Family Members and Their Effect on Wheat Quality.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Molecular Characterization of the Low Molecular Weight Glutenin Subunit Gene Family Members and Their Effect on Wheat Quality./
Author:	Ibba, Maria Itria.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2018,
Description:	183 p.
Notes:	Source: Dissertation Abstracts International, Volume: 79-11(E), Section: B.
Contained By:	Dissertation Abstracts International79-11B(E).
Subject:	Agriculture. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10785135
ISBN:	9780438104198

Molecular Characterization of the Low Molecular Weight Glutenin Subunit Gene Family Members and Their Effect on Wheat Quality.
Ibba, Maria Itria.

Molecular Characterization of the Low Molecular Weight Glutenin Subunit Gene Family Members and Their Effect on Wheat Quality. - Ann Arbor : ProQuest Dissertations & Theses, 2018 - 183 p.

Source: Dissertation Abstracts International, Volume: 79-11(E), Section: B.

Thesis (Ph.D.)--Washington State University, 2018.

Gluten is a macro polymer responsible for the unique viscoelastic properties of wheat dough and it is formed from the interaction of wheat seed storage proteins. The low-molecular-weight glutenin subunits (LMW-GSs) are one of the major components of gluten and play a critical role in the determination of wheat end-use quality. However, a clear association between different LMW-GSs and wheat quality is not well understood. The LMW-GSs are in fact hard to differentiate both at genic and protein level. They are encoded by a multigene family located on the short arm of the homoeologous group 1 chromosomes at the Glu-3 loci. The number of LMW-GS genes in each wheat variety differs and there is evidence that genes at each Glu-3 locus are divided by large intergenic and highly recombinogenic regions. Also, LMW-GSs are highly similar in structure among themselves and with many gliadins making them difficult to differentiate at the protein level. For these reasons, the identification of different LMW-GS protein profiles has been challenging, and conflicting results on the association between LMW-GS alleles and wheat end-use quality have been reported. The objective of this project was to develop a better understanding of the genetics of the LMW-GSs and of the role that each LMW-GS gene has in the determination of wheat end-use quality. First a set of common wheat varieties identified as standards for the Glu-3 alleles were analyzed for their LMW-GS genic profile to verify whether there was an association between a specific genic profile and the relative Glu-3 allele. Then, the genetic linkage between the LMW-GS genes at each Glu-3 locus was investigated to develop a better understanding of the Glu-3 loci structure. The results obtained from the two previous analyses were used to better interpret and characterize the LMW-GS profile of a set of elite bread wheat varieties and to correlate specific LMW-GS genes to different dough rheology parameters. Finally, a set of molecular markers specific for each LMW-GS haplotype was developed to facilitate the analysis of the LMW-GSs in wheat breeding programs. Results of this project will greatly improve the current knowledge of the LMW-GSs.

ISBN: 9780438104198Subjects--Topical Terms:

518588
Agriculture.

Molecular Characterization of the Low Molecular Weight Glutenin Subunit Gene Family Members and Their Effect on Wheat Quality.
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520 $a Gluten is a macro polymer responsible for the unique viscoelastic properties of wheat dough and it is formed from the interaction of wheat seed storage proteins. The low-molecular-weight glutenin subunits (LMW-GSs) are one of the major components of gluten and play a critical role in the determination of wheat end-use quality. However, a clear association between different LMW-GSs and wheat quality is not well understood. The LMW-GSs are in fact hard to differentiate both at genic and protein level. They are encoded by a multigene family located on the short arm of the homoeologous group 1 chromosomes at the Glu-3 loci. The number of LMW-GS genes in each wheat variety differs and there is evidence that genes at each Glu-3 locus are divided by large intergenic and highly recombinogenic regions. Also, LMW-GSs are highly similar in structure among themselves and with many gliadins making them difficult to differentiate at the protein level. For these reasons, the identification of different LMW-GS protein profiles has been challenging, and conflicting results on the association between LMW-GS alleles and wheat end-use quality have been reported. The objective of this project was to develop a better understanding of the genetics of the LMW-GSs and of the role that each LMW-GS gene has in the determination of wheat end-use quality. First a set of common wheat varieties identified as standards for the Glu-3 alleles were analyzed for their LMW-GS genic profile to verify whether there was an association between a specific genic profile and the relative Glu-3 allele. Then, the genetic linkage between the LMW-GS genes at each Glu-3 locus was investigated to develop a better understanding of the Glu-3 loci structure. The results obtained from the two previous analyses were used to better interpret and characterize the LMW-GS profile of a set of elite bread wheat varieties and to correlate specific LMW-GS genes to different dough rheology parameters. Finally, a set of molecular markers specific for each LMW-GS haplotype was developed to facilitate the analysis of the LMW-GSs in wheat breeding programs. Results of this project will greatly improve the current knowledge of the LMW-GSs.
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