東華大學圖書館 |

語系: 繁體中文

說明(常見問題)

回圖書館首頁

手機版館藏查詢

登入

回首頁

切換: 標籤 | MARC模式 | ISBD

Immunostimulatory Effects of Antigen...

Lyle, Chris.

FindBook

Google Book

Amazon

博客來

Immunostimulatory Effects of Antigen-Conjugated InP/ZnS Quantum Dot Nanoparticles in an Avian Model.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Immunostimulatory Effects of Antigen-Conjugated InP/ZnS Quantum Dot Nanoparticles in an Avian Model./
作者:	Lyle, Chris.
出版者:	Ann Arbor : ProQuest Dissertations & Theses, : 2018,
面頁冊數:	169 p.
附註:	Source: Dissertation Abstracts International, Volume: 79-09(E), Section: B.
Contained By:	Dissertation Abstracts International79-09B(E).
標題:	Immunology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10813694
ISBN:	9780355915518

Immunostimulatory Effects of Antigen-Conjugated InP/ZnS Quantum Dot Nanoparticles in an Avian Model.
Lyle, Chris.

Immunostimulatory Effects of Antigen-Conjugated InP/ZnS Quantum Dot Nanoparticles in an Avian Model. - Ann Arbor : ProQuest Dissertations & Theses, 2018 - 169 p.

Source: Dissertation Abstracts International, Volume: 79-09(E), Section: B.

Thesis (Ph.D.)--University of Arkansas, 2018.

Due to their unique physicochemical and enhanced immunostimulatory properties, quantum dot (QD) nanoparticles have shown increasing promise in biomedical research applications including bioimaging, drug delivery, and as vaccine adjuvants. Toxicity, however, remains a concern for the use of QD in these applications and thus, there is an increased demand for effective in vitro and in vivo systems to measure the bioactivity of QD. In this study in vitro and in vivo chicken models were used to investigate the effects of QD on innate and adaptive immunity. Chicken macrophage cultures were treated in vitro with QD to measure macrophage activation and the effect of QD on cell viability. The chicken growing feather (GF) injection model was used as the cutaneous test-site to monitor leukocyte infiltration in response to intradermal injection of QD without and with conjugation to a protein antigen. Additionally, the humoral and cellular adaptive immune responses to protein antigen conjugated to QD were examined in chickens. In vitro results showed negligible macrophage activation in response to QD treatment; however, cell viability was negatively affected by QD in a dose-dependent manner. Leukocyte infiltration results of in vivo GF injections with QD revealed immunostimulatory activity of QD, independent of whether or not QD were conjugated to protein antigen. When used as a vaccine platform, immunization with QD conjugated with protein antigen generated a higher primary and secondary antibody response compared to antigen mixed with alum adjuvant. The adaptive cellular responses to protein antigen examined in GF of immunized chickens also support an important role of B cells in the local effector phases of the responses, while T cells appear to primarily help in B cell activation and differentiation, including antibody isotype switching from IgM to IgG. These results provide additional evidence that QD possess promising vaccine adjuvant properties while demonstrating the viability and utility of the minimally invasive chicken GF cutaneous test-site to study the immunostimulatory effects of nanoparticles initiated in vivo. Further investigation of nanoparticles using the chicken GF injection model should provide additional valuable knowledge of the relative risks and rewards of using QD in biological applications.

ISBN: 9780355915518Subjects--Topical Terms:

611031
Immunology.

Immunostimulatory Effects of Antigen-Conjugated InP/ZnS Quantum Dot Nanoparticles in an Avian Model.
LDR:03333nmm a2200313 4500 001 2162940
005 20181012133446.5
008 190424s2018 ||||||||||||||||| ||eng d
020 $a 9780355915518
035 $a (MiAaPQ)AAI10813694
035 $a (MiAaPQ)uark:13005
035 $a AAI10813694
040 $a MiAaPQ $c MiAaPQ
100 1 $a Lyle, Chris. $3 3350940
245 1 0 $a Immunostimulatory Effects of Antigen-Conjugated InP/ZnS Quantum Dot Nanoparticles in an Avian Model.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2018
300 $a 169 p.
500 $a Source: Dissertation Abstracts International, Volume: 79-09(E), Section: B.
500 $a Adviser: Gisela F. Erf.
502 $a Thesis (Ph.D.)--University of Arkansas, 2018.
520 $a Due to their unique physicochemical and enhanced immunostimulatory properties, quantum dot (QD) nanoparticles have shown increasing promise in biomedical research applications including bioimaging, drug delivery, and as vaccine adjuvants. Toxicity, however, remains a concern for the use of QD in these applications and thus, there is an increased demand for effective in vitro and in vivo systems to measure the bioactivity of QD. In this study in vitro and in vivo chicken models were used to investigate the effects of QD on innate and adaptive immunity. Chicken macrophage cultures were treated in vitro with QD to measure macrophage activation and the effect of QD on cell viability. The chicken growing feather (GF) injection model was used as the cutaneous test-site to monitor leukocyte infiltration in response to intradermal injection of QD without and with conjugation to a protein antigen. Additionally, the humoral and cellular adaptive immune responses to protein antigen conjugated to QD were examined in chickens. In vitro results showed negligible macrophage activation in response to QD treatment; however, cell viability was negatively affected by QD in a dose-dependent manner. Leukocyte infiltration results of in vivo GF injections with QD revealed immunostimulatory activity of QD, independent of whether or not QD were conjugated to protein antigen. When used as a vaccine platform, immunization with QD conjugated with protein antigen generated a higher primary and secondary antibody response compared to antigen mixed with alum adjuvant. The adaptive cellular responses to protein antigen examined in GF of immunized chickens also support an important role of B cells in the local effector phases of the responses, while T cells appear to primarily help in B cell activation and differentiation, including antibody isotype switching from IgM to IgG. These results provide additional evidence that QD possess promising vaccine adjuvant properties while demonstrating the viability and utility of the minimally invasive chicken GF cutaneous test-site to study the immunostimulatory effects of nanoparticles initiated in vivo. Further investigation of nanoparticles using the chicken GF injection model should provide additional valuable knowledge of the relative risks and rewards of using QD in biological applications.
590 $a School code: 0011.
650 4 $a Immunology. $3 611031
650 4 $a Nanotechnology. $3 526235
650 4 $a Animal sciences. $3 3174829
690 $a 0982
690 $a 0652
690 $a 0475
710 2 $a University of Arkansas. $b Poultry Science. $3 2091882
773 0 $t Dissertation Abstracts International $g 79-09B(E).
790 $a 0011
791 $a Ph.D.
792 $a 2018
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10813694