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High-Throughput KASP DNA Genotyping ...

Suman, Shraddha.

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High-Throughput KASP DNA Genotyping to Select for Flowering Time among Progeny of Wild Cultivated Hybridization Populations of Chickpea.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	High-Throughput KASP DNA Genotyping to Select for Flowering Time among Progeny of Wild Cultivated Hybridization Populations of Chickpea./
作者:	Suman, Shraddha.
出版者:	Ann Arbor : ProQuest Dissertations & Theses, : 2017,
面頁冊數:	42 p.
附註:	Source: Masters Abstracts International, Volume: 56-06.
Contained By:	Masters Abstracts International56-06(E).
標題:	Plant sciences. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10265900
ISBN:	9780355149807

High-Throughput KASP DNA Genotyping to Select for Flowering Time among Progeny of Wild Cultivated Hybridization Populations of Chickpea.
Suman, Shraddha.

High-Throughput KASP DNA Genotyping to Select for Flowering Time among Progeny of Wild Cultivated Hybridization Populations of Chickpea. - Ann Arbor : ProQuest Dissertations & Theses, 2017 - 42 p.

Source: Masters Abstracts International, Volume: 56-06.

Thesis (M.S.)--University of California, Davis, 2017.

Because of the progress made in human genome SNP genotyping, high-throughput SNP genotyping is increasingly being used in plant breeding for the past decade. This project focuses on the KASP assay, which combines FRET with allele-specific amplification, to track the status of a gene that governs the flowering time trait in the chickpea plant. The Cook laboratory has identified a large-effect QTL locus, designated Dtf3-1, on chickpea linkage group 3 that controls flowering time differences among wild and cultivated chickpea, wherein the QTL region encompasses a candidate gene. In other plant species, orthologs of this candidate gene, from the Flowering locus T (FT, for short) protein family have been shown to regulate the transition to flowering. In the case of chickpea, individuals possessing the cultivated FT allele (FTc) in either the heterozygous or homozygous condition flower earlier than those with only the wild FT allele (FTw). The allelic state of the FT locus can be monitored by molecular markers from the QTL region and the candidate FT locus, which provides an opportunity to identify early flowering genotypes even in the seedling stage. DNA from F2 chickpea seeds was extracted using a crude genomic DNA extraction procedure and genotyped for the status of the FT locus using the KASP assay. The seeds were then planted and the day to first flower for each plant was observed to see if the flowering time correlated with the predicted genotype. Leaf tissue DNA from the grown plants was used to reconfirm the genotypes. The Kruskal-Wallis test was used to verify the KASP assay as a genotype predictive method.

ISBN: 9780355149807Subjects--Topical Terms:

3173832
Plant sciences.

High-Throughput KASP DNA Genotyping to Select for Flowering Time among Progeny of Wild Cultivated Hybridization Populations of Chickpea.
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