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Gene Regulatory Control of Immune Ce...

Schrankel, Catherine.

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Gene Regulatory Control of Immune Cell Specification and Differentiation in the Sea Urchin Embryo and Larva.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Gene Regulatory Control of Immune Cell Specification and Differentiation in the Sea Urchin Embryo and Larva./
Author:	Schrankel, Catherine.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2017,
Description:	219 p.
Notes:	Source: Dissertation Abstracts International, Volume: 79-03(E), Section: B.
Contained By:	Dissertation Abstracts International79-03B(E).
Subject:	Immunology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10274130
ISBN:	9780355530018

Gene Regulatory Control of Immune Cell Specification and Differentiation in the Sea Urchin Embryo and Larva.
Schrankel, Catherine.

Gene Regulatory Control of Immune Cell Specification and Differentiation in the Sea Urchin Embryo and Larva. - Ann Arbor : ProQuest Dissertations & Theses, 2017 - 219 p.

Source: Dissertation Abstracts International, Volume: 79-03(E), Section: B.

Thesis (Ph.D.)--University of Toronto (Canada), 2017.

Hematopoietic systems in mammals have been the focus of intense study. However, complications such as paralog compensation among transcription factor genes in complex vertebrate models can make it difficult to characterize gene regulatory networks (GRNs) for immune cell differentiation in detail, especially in vivo. Simple invertebrates can provide systems-level models to explore GRN function as it directs development in the intact organism. The sea urchin (Strongylocentrotus purpuratus) is an emerging model for such studies. Larval immunocytes are patterned in the mesoderm at the blastula stage. Immunocyte precursors express single gene orthologs of vertebrate hematopoietic factors, including GATA-1,-2, 3 factors, Scl/Tal2/Lyl1, and the E-proteins (E2A/HEB/E2-2). Genome analyses also reveal a complex system of immune receptors and effectors.

ISBN: 9780355530018Subjects--Topical Terms:

611031
Immunology.

Gene Regulatory Control of Immune Cell Specification and Differentiation in the Sea Urchin Embryo and Larva.
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245 1 0 $a Gene Regulatory Control of Immune Cell Specification and Differentiation in the Sea Urchin Embryo and Larva.
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300 $a 219 p.
500 $a Source: Dissertation Abstracts International, Volume: 79-03(E), Section: B.
500 $a Adviser: Jonathan P. Rast.
502 $a Thesis (Ph.D.)--University of Toronto (Canada), 2017.
520 $a Hematopoietic systems in mammals have been the focus of intense study. However, complications such as paralog compensation among transcription factor genes in complex vertebrate models can make it difficult to characterize gene regulatory networks (GRNs) for immune cell differentiation in detail, especially in vivo. Simple invertebrates can provide systems-level models to explore GRN function as it directs development in the intact organism. The sea urchin (Strongylocentrotus purpuratus) is an emerging model for such studies. Larval immunocytes are patterned in the mesoderm at the blastula stage. Immunocyte precursors express single gene orthologs of vertebrate hematopoietic factors, including GATA-1,-2, 3 factors, Scl/Tal2/Lyl1, and the E-proteins (E2A/HEB/E2-2). Genome analyses also reveal a complex system of immune receptors and effectors.
520 $a In my thesis I sought to 1) characterize immune cell terminal differentiation genes in more detail, 2) characterize the role of E-protein transcription factor splice variants driving the emergence of distinct mesodermal cell types, and 3) characterize the later expression of key transcription factors in maintaining larval immunocyte populations. I identified two markers: a thioester ?-macroglobulin like protein, Tecp2, and MacpfA2. MacpfA2 is part of a multi-gene family of 22 perforin-related effectors. Phylogenetic and expression analyses of the Macpf family identified eight sub-families that exhibit differential expression domains and transcriptional responses to infection across embryonic and adult tissues and cells.
520 $a Two isoforms of SpE-protein were identified in immunocyte precursors: a longer canonical (Can) form and a truncated alternative (Alt) form that initiates from a unique first exon. SpE-Alt shares genomic organization and sequence conservation with the HEB and E2-2 vertebrate paralogs. SpE-Alt is required for immunocyte development and functions in a novel cis-regulatory mechanism between the two isoforms. A second isoform of SpGATA123 was also identified. A short isoform is predominant in immunocyte precursors and adult coelomocytes, whereas a longer form is expressed in larval endoderm. SpGATA123-S perturbation affects immunoctye migration and the expression of MacpfA2 . SpGATA123-S and other early regulators of immunocyte specification re-emerge in the coelomic pouches of the feeding larva.
520 $a These studies in the sea urchin have reinforced the hypothesis that although cell morphology and terminal effectors diversify rapidly among different animals, deep homology exists at the level of primitive hemocyte development across deuterostomes. Aspects of these systems are re-deployed in definitive stages of hematopoiesis. In sum, simple animal models offer tractable systems for studying the regulatory machinery governing immune cell emergence in vivo. This provides a foundation for comparative GRN investigations that will improve our understanding of the evolution of immune systems across phyla.
590 $a School code: 0779.
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650 4 $a Developmental biology. $3 592588
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