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Quantitative analysis of the total b...

Jung, Deborah Osterholm.

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Quantitative analysis of the total bacteria, Lactobacillus, and Bifidobacterium colonic microflora in rats fed conventional, prebiotic, and probiotic soy diets.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Quantitative analysis of the total bacteria, Lactobacillus, and Bifidobacterium colonic microflora in rats fed conventional, prebiotic, and probiotic soy diets./
Author:	Jung, Deborah Osterholm.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2015,
Description:	65 p.
Notes:	Source: Masters Abstracts International, Volume: 55-03.
Contained By:	Masters Abstracts International55-03(E).
Subject:	Nutrition. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10012779
ISBN:	9781339477251

Quantitative analysis of the total bacteria, Lactobacillus, and Bifidobacterium colonic microflora in rats fed conventional, prebiotic, and probiotic soy diets.
Jung, Deborah Osterholm.

Quantitative analysis of the total bacteria, Lactobacillus, and Bifidobacterium colonic microflora in rats fed conventional, prebiotic, and probiotic soy diets. - Ann Arbor : ProQuest Dissertations & Theses, 2015 - 65 p.

Source: Masters Abstracts International, Volume: 55-03.

Thesis (M.S.)--Southern Illinois University at Carbondale, 2015.

Research suggests that specific compositions of gut microbiota can directly affect energy harvesting and fat storage, which may indicate a potential role of intestinal bacteria in the regulation of body weight (i.e., obesity). The purpose of the current study was to determine if prebiotic- and probiotic-based diets modify gut microbiota in genetically obese rodents. For this, female Zucker diabetic fatty (ZDF) rats were assigned diets containing fructooligosaccharides (FOS), Bifidobacterium (BIF), or Lactobacillus (LAC) for three weeks. qPCR was then used to measure levels of colonic Bifidobacterium, Lactobacillus, and total bacteria. At termination, there was no significant difference in Lactobacillus levels between diets. However, there was significantly less Bifidobacterium in BIF vs. FOS or LAC-fed rats. The evidence in this study shows there were no significant differences in Lactobacillus levels between any of the feeding groups and the control group, supporting the conclusion that ingestion of any of the tested supplemented feed does not statistically modulate Lactobacillus numbers in female ZDF rats. However, the rats from the Bifidobacterium and FOS feeding groups had significantly higher colonic Bifidobacterium levels than the control group from ingesting the supplemented feed, indicating that the presence of the probiotic Bifidobacterium animalis subspecies lactis and the prebiotic FOS stimulated the growth of Bifidobacterium.

ISBN: 9781339477251Subjects--Topical Terms:

517777
Nutrition.

Quantitative analysis of the total bacteria, Lactobacillus, and Bifidobacterium colonic microflora in rats fed conventional, prebiotic, and probiotic soy diets.
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100 1 $a Jung, Deborah Osterholm. $3 3289436
245 1 0 $a Quantitative analysis of the total bacteria, Lactobacillus, and Bifidobacterium colonic microflora in rats fed conventional, prebiotic, and probiotic soy diets.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2015
300 $a 65 p.
500 $a Source: Masters Abstracts International, Volume: 55-03.
500 $a Adviser: Jeremy E. Davis.
502 $a Thesis (M.S.)--Southern Illinois University at Carbondale, 2015.
520 $a Research suggests that specific compositions of gut microbiota can directly affect energy harvesting and fat storage, which may indicate a potential role of intestinal bacteria in the regulation of body weight (i.e., obesity). The purpose of the current study was to determine if prebiotic- and probiotic-based diets modify gut microbiota in genetically obese rodents. For this, female Zucker diabetic fatty (ZDF) rats were assigned diets containing fructooligosaccharides (FOS), Bifidobacterium (BIF), or Lactobacillus (LAC) for three weeks. qPCR was then used to measure levels of colonic Bifidobacterium, Lactobacillus, and total bacteria. At termination, there was no significant difference in Lactobacillus levels between diets. However, there was significantly less Bifidobacterium in BIF vs. FOS or LAC-fed rats. The evidence in this study shows there were no significant differences in Lactobacillus levels between any of the feeding groups and the control group, supporting the conclusion that ingestion of any of the tested supplemented feed does not statistically modulate Lactobacillus numbers in female ZDF rats. However, the rats from the Bifidobacterium and FOS feeding groups had significantly higher colonic Bifidobacterium levels than the control group from ingesting the supplemented feed, indicating that the presence of the probiotic Bifidobacterium animalis subspecies lactis and the prebiotic FOS stimulated the growth of Bifidobacterium.
590 $a School code: 0209.
650 4 $a Nutrition. $3 517777
650 4 $a Food science. $3 3173303
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