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Wavelength- and polarization-depende...

Campbell, Kirby Robert.

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Wavelength- and polarization-dependent second harmonic generation imaging microscopy and development of a nonlinear tomography platform for characterizing collagen assembly.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Wavelength- and polarization-dependent second harmonic generation imaging microscopy and development of a nonlinear tomography platform for characterizing collagen assembly./
Author:	Campbell, Kirby Robert.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2017,
Description:	165 p.
Notes:	Source: Dissertation Abstracts International, Volume: 78-10(E), Section: B.
Contained By:	Dissertation Abstracts International78-10B(E).
Subject:	Biomedical engineering. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10282585
ISBN:	9781369770537

Wavelength- and polarization-dependent second harmonic generation imaging microscopy and development of a nonlinear tomography platform for characterizing collagen assembly.
Campbell, Kirby Robert.

Wavelength- and polarization-dependent second harmonic generation imaging microscopy and development of a nonlinear tomography platform for characterizing collagen assembly. - Ann Arbor : ProQuest Dissertations & Theses, 2017 - 165 p.

Source: Dissertation Abstracts International, Volume: 78-10(E), Section: B.

Thesis (Ph.D.)--The University of Wisconsin - Madison, 2017.

The use of second harmonic generation (SHG) imaging has provided a key role in studying a variety of ordered systems, such as complex surfaces, muscle, extracellular matrix (ECM) and other chiral materials. Moreover, the continued technological advancements of turnkey ultrafast laser systems have enabled smaller, less expensive commercial SHG imaging systems. Consequently, this miniaturization has allowed for more widespread clinical diagnostic and prognostic use of SHG imaging further fueling the need of development of robust techniques for measuring morphological attributes pertaining to normal and abnormal tissues. This thesis focuses on advancing these techniques on benchtop instruments and baselining SHG emission responses from a variety of ex vivo human tissue and tissue-model self-polymerizing collagen gels.

ISBN: 9781369770537Subjects--Topical Terms:

535387
Biomedical engineering.

Wavelength- and polarization-dependent second harmonic generation imaging microscopy and development of a nonlinear tomography platform for characterizing collagen assembly.
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245 1 0 $a Wavelength- and polarization-dependent second harmonic generation imaging microscopy and development of a nonlinear tomography platform for characterizing collagen assembly.
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300 $a 165 p.
500 $a Source: Dissertation Abstracts International, Volume: 78-10(E), Section: B.
500 $a Adviser: Paul J. Campagnola.
502 $a Thesis (Ph.D.)--The University of Wisconsin - Madison, 2017.
520 $a The use of second harmonic generation (SHG) imaging has provided a key role in studying a variety of ordered systems, such as complex surfaces, muscle, extracellular matrix (ECM) and other chiral materials. Moreover, the continued technological advancements of turnkey ultrafast laser systems have enabled smaller, less expensive commercial SHG imaging systems. Consequently, this miniaturization has allowed for more widespread clinical diagnostic and prognostic use of SHG imaging further fueling the need of development of robust techniques for measuring morphological attributes pertaining to normal and abnormal tissues. This thesis focuses on advancing these techniques on benchtop instruments and baselining SHG emission responses from a variety of ex vivo human tissue and tissue-model self-polymerizing collagen gels.
520 $a First, ovarian tissue architecture is characterized both locally and non-locally using SHG creation physics and optical scattering measurements based on wavelength dependence. Second, polarization-resolved SHG methods were advanced. These efforts include standardization of SHG-circular dichroism effects for various isoforms in tissue models by measuring differing concentrations of Col III/I self-polymerizing gel samples. Ovarian tissue collagen hierarchy assembly is then characterized via linear polarization and SHG-CD analyses. Lastly, a full-3D SHG imaging approach is proposed. A novel, nonlinear imaging platform laterally rotates specimens for multi-view acquisition. Multiple image stacks from different angles allows probing collagen fibers at various orientations relative to a fixed laser excitation. Subsequent registration and reconstruction of the views enable full visualization of all fibers in collagen-rich tissue specimens. These advancements of SHG imaging techniques and the development of true-3D SHG imaging may enhance the accuracy of current SHG imaging analytic strategies allowing for optimization of benchtop tissue characterizations to be later used as foundation of knowledge for potential clinical instruments.
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