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Serotonin Modulates a Calcium-Driven...

Zahratka, Jeffrey A.

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Serotonin Modulates a Calcium-Driven Negative Feedback Loop in a C. elegans Nociceptor.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Serotonin Modulates a Calcium-Driven Negative Feedback Loop in a C. elegans Nociceptor./
作者:	Zahratka, Jeffrey A.
出版者:	Ann Arbor : ProQuest Dissertations & Theses, : 2015,
面頁冊數:	126 p.
附註:	Source: Dissertation Abstracts International, Volume: 78-06(E), Section: B.
Contained By:	Dissertation Abstracts International78-06B(E).
標題:	Neurobiology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10145313
ISBN:	9781369008456

Serotonin Modulates a Calcium-Driven Negative Feedback Loop in a C. elegans Nociceptor.
Zahratka, Jeffrey A.

Serotonin Modulates a Calcium-Driven Negative Feedback Loop in a C. elegans Nociceptor. - Ann Arbor : ProQuest Dissertations & Theses, 2015 - 126 p.

Source: Dissertation Abstracts International, Volume: 78-06(E), Section: B.

Thesis (Ph.D.)--The University of Toledo, 2015.

Neuromodulation in sensory circuits is critical because it allows an organism to respond appropriately to a given stimulus. Sensory systems are modulated by monoamine neurotransmitters as well as neuropeptides, which act in concert to regulate sensory circuits to give rise to complex behavioral states. One common technique for studying sensory circuits is brain activity mapping, where circuits are probed with fluorescent indicators whose readouts are related directly to neuronal activity. In the present work, we focus on the modulation of a pair of sensory neurons, the ASHs, in the nematode Caenorhabditis elegans. ASHs are polymodal, nociceptive neurons that are extensively modulated by monoamines and neuropeptides. Using a combination of genetics, Ca2+ imaging, electrophysiology, and behavioral assays, we have identified a complex instance where the monoamine serotonin (5-HT) stimulates aversive behaviors and neuronal depolarization, but decreases sensory-evoked Ca2+ signals, indicating that the recorded Ca 2+ levels do not positively correlate with neuronal activity. Mechanistically, 5-HT is likely acting through the SER-5 receptor and Galphaq signaling in ASHs to downregulate Ca2+ directly by initiating a Ca2+-driven negative feedback loop targeting the L-type Ca 2+ channel EGL-19. Together, these studies reveal a complex inhibitory feedback mechanism for sensory modulation, and have broad implications for activity mapping of complex neural circuits.

ISBN: 9781369008456Subjects--Topical Terms:

588707
Neurobiology.

Serotonin Modulates a Calcium-Driven Negative Feedback Loop in a C. elegans Nociceptor.
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