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DNA polymerase exchange and lesion b...

Kath, James Evon.

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DNA polymerase exchange and lesion bypass in Escherichia coli.

Record Type:	Electronic resources : Monograph/item
Title/Author:	DNA polymerase exchange and lesion bypass in Escherichia coli./
Author:	Kath, James Evon.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2016,
Description:	131 p.
Notes:	Source: Dissertation Abstracts International, Volume: 77-09(E), Section: B.
Contained By:	Dissertation Abstracts International77-09B(E).
Subject:	Biophysics. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10102867
ISBN:	9781339664958

DNA polymerase exchange and lesion bypass in Escherichia coli.
Kath, James Evon.

DNA polymerase exchange and lesion bypass in Escherichia coli. - Ann Arbor : ProQuest Dissertations & Theses, 2016 - 131 p.

Source: Dissertation Abstracts International, Volume: 77-09(E), Section: B.

Thesis (Ph.D.)--Harvard University, 2016.

This item is not available from ProQuest Dissertations & Theses.

Translesion synthesis (TLS) alleviates replication stalling at DNA lesions. Bypass of lesions by specialized translesion DNA polymerases involves exchange with high-fidelity replicative polymerases. As a consequence of their lesion bypass activity, TLS polymerases are mutagenic, requiring careful regulation of polymerase selection. In this dissertation, I describe a single-molecule reconstitution of polymerase exchange and lesion bypass. Using Escherichia coli polymerases as a model system, I have determined that the dimeric processivity clamp can simultaneously bind a replicative polymerase and a translesion polymerase, facilitating rapid exchange during synthesis and lesion bypass. Overlapping sets of polymerase-clamp interactions additionally allow the TLS polymerase Polymerase IV to displace the replicative polymerase Polymerase III. I finally describe the observation of single Polymerase IV molecules in living cells and initial efforts to determine their localization and dynamics during TLS.

ISBN: 9781339664958Subjects--Topical Terms:

518360
Biophysics.

DNA polymerase exchange and lesion bypass in Escherichia coli.
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245 1 0 $a DNA polymerase exchange and lesion bypass in Escherichia coli.
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300 $a 131 p.
500 $a Source: Dissertation Abstracts International, Volume: 77-09(E), Section: B.
500 $a Adviser: James M. Hogle.
502 $a Thesis (Ph.D.)--Harvard University, 2016.
506 $a This item is not available from ProQuest Dissertations & Theses.
520 $a Translesion synthesis (TLS) alleviates replication stalling at DNA lesions. Bypass of lesions by specialized translesion DNA polymerases involves exchange with high-fidelity replicative polymerases. As a consequence of their lesion bypass activity, TLS polymerases are mutagenic, requiring careful regulation of polymerase selection. In this dissertation, I describe a single-molecule reconstitution of polymerase exchange and lesion bypass. Using Escherichia coli polymerases as a model system, I have determined that the dimeric processivity clamp can simultaneously bind a replicative polymerase and a translesion polymerase, facilitating rapid exchange during synthesis and lesion bypass. Overlapping sets of polymerase-clamp interactions additionally allow the TLS polymerase Polymerase IV to displace the replicative polymerase Polymerase III. I finally describe the observation of single Polymerase IV molecules in living cells and initial efforts to determine their localization and dynamics during TLS.
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650 4 $a Biophysics. $3 518360
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