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Nonlinear optical methods for the an...

Dow, Ximeng You.

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Nonlinear optical methods for the analysis of protein nanocrystals and biological tissues.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Nonlinear optical methods for the analysis of protein nanocrystals and biological tissues./
Author:	Dow, Ximeng You.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2016,
Description:	251 p.
Notes:	Source: Dissertation Abstracts International, Volume: 78-02(E), Section: B.
Contained By:	Dissertation Abstracts International78-02B(E).
Subject:	Analytical chemistry. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10149319
ISBN:	9781369044379

Nonlinear optical methods for the analysis of protein nanocrystals and biological tissues.
Dow, Ximeng You.

Nonlinear optical methods for the analysis of protein nanocrystals and biological tissues. - Ann Arbor : ProQuest Dissertations & Theses, 2016 - 251 p.

Source: Dissertation Abstracts International, Volume: 78-02(E), Section: B.

Thesis (Ph.D.)--Purdue University, 2016.

Structural biology underpins rational drug design and fundamental understanding of protein function. X-ray diffraction (XRD) has been the golden standard for solving for high-resolution protein structure. Second harmonic generation (SHG) microscopy has been developed by the Simpson lab as a sensitive, crystal-specific detection method for the identification of protein crystal and help optimize the crystallization condition. Protein nanocrystals has been widely used for structure determination of membrane proteins in serial femtosecond nanocrystallography. In this thesis work, novel nonlinear optical methods were developed to address the challenges associated with the detection and characterization of protein nanocrystals. SHG-correlation spectroscopy (SHG-CS) was developed to take advantage of the diffusing motion and retrieve the size distribution and crystal quality of the nanocrystals. Polarization-dependent SHG imaging technique was developed to measure the relative orientation as well as the internal structure of the sample. Two photon- excited fluorescence has been used in the Simpson lab as a complementary measurement besides the inherent SHG signal from the crystals. A novel instrumentation development was also introduced in this thesis work to greatly improve the speed of fluorescence lifetime imaging (FLIM).

ISBN: 9781369044379Subjects--Topical Terms:

3168300
Analytical chemistry.

Nonlinear optical methods for the analysis of protein nanocrystals and biological tissues.
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100 1 $a Dow, Ximeng You. $3 3281832
245 1 0 $a Nonlinear optical methods for the analysis of protein nanocrystals and biological tissues.
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300 $a 251 p.
500 $a Source: Dissertation Abstracts International, Volume: 78-02(E), Section: B.
500 $a Adviser: Garth J. Simpson.
502 $a Thesis (Ph.D.)--Purdue University, 2016.
520 $a Structural biology underpins rational drug design and fundamental understanding of protein function. X-ray diffraction (XRD) has been the golden standard for solving for high-resolution protein structure. Second harmonic generation (SHG) microscopy has been developed by the Simpson lab as a sensitive, crystal-specific detection method for the identification of protein crystal and help optimize the crystallization condition. Protein nanocrystals has been widely used for structure determination of membrane proteins in serial femtosecond nanocrystallography. In this thesis work, novel nonlinear optical methods were developed to address the challenges associated with the detection and characterization of protein nanocrystals. SHG-correlation spectroscopy (SHG-CS) was developed to take advantage of the diffusing motion and retrieve the size distribution and crystal quality of the nanocrystals. Polarization-dependent SHG imaging technique was developed to measure the relative orientation as well as the internal structure of the sample. Two photon- excited fluorescence has been used in the Simpson lab as a complementary measurement besides the inherent SHG signal from the crystals. A novel instrumentation development was also introduced in this thesis work to greatly improve the speed of fluorescence lifetime imaging (FLIM).
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650 4 $a Analytical chemistry. $3 3168300
650 4 $a Optics. $3 517925
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710 2 $a Purdue University. $b Chemistry. $3 1019176
773 0 $t Dissertation Abstracts International $g 78-02B(E).
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856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10149319