東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back

Switch To: Labeled | MARC Mode | ISBD

A Competition Mechanism for a Homeot...

Gordon, Patricia M.

Linked to FindBook

Google Book

Amazon

博客來

A Competition Mechanism for a Homeotic Neuron Identity Transformation in Caenorhabditis elegans.

Record Type:	Electronic resources : Monograph/item
Title/Author:	A Competition Mechanism for a Homeotic Neuron Identity Transformation in Caenorhabditis elegans./
Author:	Gordon, Patricia M.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2015,
Description:	210 p.
Notes:	Source: Dissertation Abstracts International, Volume: 76-04(E), Section: B.
Contained By:	Dissertation Abstracts International76-04B(E).
Subject:	Developmental biology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3665587
ISBN:	9781321383089

A Competition Mechanism for a Homeotic Neuron Identity Transformation in Caenorhabditis elegans.
Gordon, Patricia M.

A Competition Mechanism for a Homeotic Neuron Identity Transformation in Caenorhabditis elegans. - Ann Arbor : ProQuest Dissertations & Theses, 2015 - 210 p.

Source: Dissertation Abstracts International, Volume: 76-04(E), Section: B.

Thesis (Ph.D.)--Columbia University, 2015.

This item is not available from ProQuest Dissertations & Theses.

As embryos proceed through development, they must undergo a series of cell fate decisions. At each division, potency is progressively restricted until a terminally differentiated, postmitotic cell is produced. An important part of that cell type determination is repression of alternative fate possibilities. In this thesis, I have explored the mechanisms by which a single transcription factor activates certain cell fates while inhibiting others, using the Caenorhabditis elegans ALM and BDU neurons as a model. ALM neuron identity is regulated by two interacting transcription factors: the POU homeobox gene unc-86 and the LIM homeobox gene mec-3. I investigated fate determination in BDU neurons, the sister cells of ALM. I found that BDU identity is broadly defined by a combination of unc-86 and the Zn finger transcription factor pag-3, while the neuropeptidergic subroutine of BDU is determined by the LIM homeobox gene ceh-14. In addition, I found that reciprocal homeotic transformations occur between ALM and BDU neurons upon loss of either mec-3 or pag-3. In mec-3 mutants, ALM neurons acquire the gene expression profile and morphological characteristics of BDU cells, while in pag-3 mutants, BDU neurons express genes normally found in ALM and change some aspects of their morphology to resemble ALM. While these fate switches appear to be a simple case of cross-repression, the mechanism is in fact more complicated, as pag-3 is expressed not just in BDU but also in ALM. In this thesis, I present evidence that MEC-3 inhibits execution of BDU identity in ALM by physically binding to UNC-86 and sequestering it away from the promoters of BDU genes. This work expands upon the literature examining simultaneous activation of one identity program and repression of alternate programs by introducing a novel mechanism by which a transcription factor competes to direct specific cell fates.

ISBN: 9781321383089Subjects--Topical Terms:

592588
Developmental biology.

A Competition Mechanism for a Homeotic Neuron Identity Transformation in Caenorhabditis elegans.
LDR:02963nmm a2200325 4500 001 2117594
005 20170530090102.5
008 180830s2015 ||||||||||||||||| ||eng d
020 $a 9781321383089
035 $a (MiAaPQ)AAI3665587
035 $a AAI3665587
040 $a MiAaPQ $c MiAaPQ
100 1 $a Gordon, Patricia M. $3 3279374
245 1 2 $a A Competition Mechanism for a Homeotic Neuron Identity Transformation in Caenorhabditis elegans.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2015
300 $a 210 p.
500 $a Source: Dissertation Abstracts International, Volume: 76-04(E), Section: B.
500 $a Adviser: Oliver Hobert.
502 $a Thesis (Ph.D.)--Columbia University, 2015.
506 $a This item is not available from ProQuest Dissertations & Theses.
520 $a As embryos proceed through development, they must undergo a series of cell fate decisions. At each division, potency is progressively restricted until a terminally differentiated, postmitotic cell is produced. An important part of that cell type determination is repression of alternative fate possibilities. In this thesis, I have explored the mechanisms by which a single transcription factor activates certain cell fates while inhibiting others, using the Caenorhabditis elegans ALM and BDU neurons as a model. ALM neuron identity is regulated by two interacting transcription factors: the POU homeobox gene unc-86 and the LIM homeobox gene mec-3. I investigated fate determination in BDU neurons, the sister cells of ALM. I found that BDU identity is broadly defined by a combination of unc-86 and the Zn finger transcription factor pag-3, while the neuropeptidergic subroutine of BDU is determined by the LIM homeobox gene ceh-14. In addition, I found that reciprocal homeotic transformations occur between ALM and BDU neurons upon loss of either mec-3 or pag-3. In mec-3 mutants, ALM neurons acquire the gene expression profile and morphological characteristics of BDU cells, while in pag-3 mutants, BDU neurons express genes normally found in ALM and change some aspects of their morphology to resemble ALM. While these fate switches appear to be a simple case of cross-repression, the mechanism is in fact more complicated, as pag-3 is expressed not just in BDU but also in ALM. In this thesis, I present evidence that MEC-3 inhibits execution of BDU identity in ALM by physically binding to UNC-86 and sequestering it away from the promoters of BDU genes. This work expands upon the literature examining simultaneous activation of one identity program and repression of alternate programs by introducing a novel mechanism by which a transcription factor competes to direct specific cell fates.
590 $a School code: 0054.
650 4 $a Developmental biology. $3 592588
650 4 $a Evolution & development. $3 3172418
650 4 $a Cellular biology. $3 3172791
650 4 $a Neurosciences. $3 588700
690 $a 0758
690 $a 0412
690 $a 0379
690 $a 0317
710 2 $a Columbia University. $b Biochemistry and Molecular Biophysics. $3 3276398
773 0 $t Dissertation Abstracts International $g 76-04B(E).
790 $a 0054
791 $a Ph.D.
792 $a 2015
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3665587