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Development of solid phase immunoext...

Grant, Gordon Alan.

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Development of solid phase immunoextraction matrix-assisted laser/desorption time-of-flight mass spectrometry for improving analysis efficiency of sulfonamide residues in environmental samples.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Development of solid phase immunoextraction matrix-assisted laser/desorption time-of-flight mass spectrometry for improving analysis efficiency of sulfonamide residues in environmental samples./
作者:	Grant, Gordon Alan.
出版者:	Ann Arbor : ProQuest Dissertations & Theses, : 2004,
面頁冊數:	282 p.
附註:	Source: Dissertation Abstracts International, Volume: 65-10, Section: B, page: 4912.
Contained By:	Dissertation Abstracts International65-10B.
標題:	Food science. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=NQ95939
ISBN:	9780612959392

Development of solid phase immunoextraction matrix-assisted laser/desorption time-of-flight mass spectrometry for improving analysis efficiency of sulfonamide residues in environmental samples.
Grant, Gordon Alan.

Development of solid phase immunoextraction matrix-assisted laser/desorption time-of-flight mass spectrometry for improving analysis efficiency of sulfonamide residues in environmental samples. - Ann Arbor : ProQuest Dissertations & Theses, 2004 - 282 p.

Source: Dissertation Abstracts International, Volume: 65-10, Section: B, page: 4912.

Thesis (Ph.D.)--University of Alberta (Canada), 2004.

The main objective of this research was to improve the speed of mass spectrometric analysis of pollutant residues in environmental samples without compromising sensitivity or accuracy. Humanity produces and uses a growing quantity and variety of chemicals that reach the ecosystem with mostly unknown long-term fates and consequences. Sulfamethazine (SMT) is a synthetic, chemically-stable, antibacterial compound, and served as a model for the development of a qualitative detection system for complex matrices, such as food or environmental samples. SMT is routinely used in animal husbandry to prevent disease, and occasionally, violative residues can be found in milk, eggs, or meat, and it has been detected in waterway surveys.

ISBN: 9780612959392Subjects--Topical Terms:

3173303
Food science.

Development of solid phase immunoextraction matrix-assisted laser/desorption time-of-flight mass spectrometry for improving analysis efficiency of sulfonamide residues in environmental samples.
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500 $a Source: Dissertation Abstracts International, Volume: 65-10, Section: B, page: 4912.
500 $a Adviser: Peter Sporns.
502 $a Thesis (Ph.D.)--University of Alberta (Canada), 2004.
520 $a The main objective of this research was to improve the speed of mass spectrometric analysis of pollutant residues in environmental samples without compromising sensitivity or accuracy. Humanity produces and uses a growing quantity and variety of chemicals that reach the ecosystem with mostly unknown long-term fates and consequences. Sulfamethazine (SMT) is a synthetic, chemically-stable, antibacterial compound, and served as a model for the development of a qualitative detection system for complex matrices, such as food or environmental samples. SMT is routinely used in animal husbandry to prevent disease, and occasionally, violative residues can be found in milk, eggs, or meat, and it has been detected in waterway surveys.
520 $a A novel drug residue detection system was developed, called solid phase immunoextraction (SPIE) matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS). SPIE recovered SMT and its N4-acetyl metabolite (NA-SMT), and was comprised of a primary solid phase extraction (SPE) cartridge for concentration, then a high capacity anti-SMT immunoaffinity column (IAC) for purification, and lastly, a micro-SPE concentration step. The SPIE MALDI-TOF MS unequivocally detected trace-levels of SMT and NA-SMT in fortified water (0.1 ng/L), soil (12 ng/kg) and manure (15 ng/kg) samples or incurred in field samples from normal farm practices of spreading pig manure on the land. Quantitative estimates for field samples were made relative to an N4-proprionyl-SMT internal standard. Improvements are required to validate this as a quantitative method due to inconsistent recoveries at the primary SPE stage for soil and manure samples (20--180%). The whole process took less than 2 hours per 8-sample batch, representing two or 3-fold improvement in analysis efficiency compared to other conventional confirmatory MS methodologies. The high throughput potential of immuno-MALDI-TOF MS systems and their future of residue analysis are discussed.
520 $a The chemical nature of the sulfonamide-antibody interaction was partly elucidated by testing various sulfonamide analogues and derivatives in competitive enzyme-linked immunosorbent assay (ELISA). These experiments, which used polyclonal anti-sulfonamide sera raised using different sulfonamide immunogens, described a possible antibody screening system for IAC applications, where ELISA results (affinity, specificity, immunogen linker arm effects) can be correlated to subsequent IAC performance.
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