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Synthesis and optical properties of ...

Pitter, Demar R. G.

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Synthesis and optical properties of novel turn-on, fluorescent nuclear stains with live cell compatibility.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Synthesis and optical properties of novel turn-on, fluorescent nuclear stains with live cell compatibility./
作者:	Pitter, Demar R. G.
面頁冊數:	144 p.
附註:	Source: Dissertation Abstracts International, Volume: 77-10(E), Section: B.
Contained By:	Dissertation Abstracts International77-10B(E).
標題:	Analytical chemistry. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10110373
ISBN:	9781339736280

Synthesis and optical properties of novel turn-on, fluorescent nuclear stains with live cell compatibility.
Pitter, Demar R. G.

Synthesis and optical properties of novel turn-on, fluorescent nuclear stains with live cell compatibility. - 144 p.

Source: Dissertation Abstracts International, Volume: 77-10(E), Section: B.

Thesis (Ph.D.)--University of Miami, 2016.

Fluorophores are an important class of compounds that are used as tools to better understand the processes that occur in cells. A subset of biologically useful fluorophores are nucleic acid binding probes. The inherent problems of many of the nucleic acid binding probes used on the market today is three-fold; (1) that the probes used produce some amount of autofluorescence from the biological sample due to the excitation wavelength needed for them to emit, (2) that most of the common probes lack cell membrane permeability, thus requiring fixation and permeabilization which limits the ability to observe dynamic processes of the biological sample and (3) that probes tend to have excitation maxima that poorly match commonly used visible laser lines. This research focuses on synthesizing probes that overcome these limitations by: exploiting the twisted intramolecular charge transfer (TICT) phenomena of probes to produce high on/off ratios when bound to nucleic acids, are membrane permeable, are live cell compatible, and match the major laser lines available for microscopy. Furthermore, with help from the development of these probes a new method for detecting DNA binding modes has been established through the use of two-photon spectroscopy.

ISBN: 9781339736280Subjects--Topical Terms:

3168300
Analytical chemistry.

Synthesis and optical properties of novel turn-on, fluorescent nuclear stains with live cell compatibility.
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520 $a Fluorophores are an important class of compounds that are used as tools to better understand the processes that occur in cells. A subset of biologically useful fluorophores are nucleic acid binding probes. The inherent problems of many of the nucleic acid binding probes used on the market today is three-fold; (1) that the probes used produce some amount of autofluorescence from the biological sample due to the excitation wavelength needed for them to emit, (2) that most of the common probes lack cell membrane permeability, thus requiring fixation and permeabilization which limits the ability to observe dynamic processes of the biological sample and (3) that probes tend to have excitation maxima that poorly match commonly used visible laser lines. This research focuses on synthesizing probes that overcome these limitations by: exploiting the twisted intramolecular charge transfer (TICT) phenomena of probes to produce high on/off ratios when bound to nucleic acids, are membrane permeable, are live cell compatible, and match the major laser lines available for microscopy. Furthermore, with help from the development of these probes a new method for detecting DNA binding modes has been established through the use of two-photon spectroscopy.
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