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New Approach to Selective Stem Cell ...

Kisa, Fikrullah.

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New Approach to Selective Stem Cell Sorting: Separation of Undifferentiated Stem Cells from Differentiated Stem Cells by Using Iron Oxide Core Shell Nanoparticles.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	New Approach to Selective Stem Cell Sorting: Separation of Undifferentiated Stem Cells from Differentiated Stem Cells by Using Iron Oxide Core Shell Nanoparticles./
作者:	Kisa, Fikrullah.
面頁冊數:	173 p.
附註:	Source: Dissertation Abstracts International, Volume: 77-05(E), Section: B.
Contained By:	Dissertation Abstracts International77-05B(E).
標題:	Animal sciences. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3741455
ISBN:	9781339337418

New Approach to Selective Stem Cell Sorting: Separation of Undifferentiated Stem Cells from Differentiated Stem Cells by Using Iron Oxide Core Shell Nanoparticles.
Kisa, Fikrullah.

New Approach to Selective Stem Cell Sorting: Separation of Undifferentiated Stem Cells from Differentiated Stem Cells by Using Iron Oxide Core Shell Nanoparticles. - 173 p.

Source: Dissertation Abstracts International, Volume: 77-05(E), Section: B.

Thesis (Ph.D.)--The University of Wisconsin - Madison, 2015.

An alternative approach to stem cell enrichment in another words sorting methods without changing the microenvironment of the cells to avoid the detrimental effects of present cell sorting methods by adopting iron-oxide gold (cFeAu) core-shell nanoparticles (NPs) is the focus of this thesis. Each chapter of this thesis focuses on different preliminary research in order to engender the adoption of cFeAu NPs for the selective killing of the mouse embryonic stem cells that are immunolabeled with the nanoparticles.

ISBN: 9781339337418Subjects--Topical Terms:

3174829
Animal sciences.

New Approach to Selective Stem Cell Sorting: Separation of Undifferentiated Stem Cells from Differentiated Stem Cells by Using Iron Oxide Core Shell Nanoparticles.
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245 1 0 $a New Approach to Selective Stem Cell Sorting: Separation of Undifferentiated Stem Cells from Differentiated Stem Cells by Using Iron Oxide Core Shell Nanoparticles.
300 $a 173 p.
500 $a Source: Dissertation Abstracts International, Volume: 77-05(E), Section: B.
500 $a Adviser: Ralph M. Albrecht.
502 $a Thesis (Ph.D.)--The University of Wisconsin - Madison, 2015.
520 $a An alternative approach to stem cell enrichment in another words sorting methods without changing the microenvironment of the cells to avoid the detrimental effects of present cell sorting methods by adopting iron-oxide gold (cFeAu) core-shell nanoparticles (NPs) is the focus of this thesis. Each chapter of this thesis focuses on different preliminary research in order to engender the adoption of cFeAu NPs for the selective killing of the mouse embryonic stem cells that are immunolabeled with the nanoparticles.
520 $a The first part of the research focuses on the synthesis of superparamagnetic iron-oxide nanoparticles with the co-precipitation method and coating the nanoparticles with colloidal gold (cAu) to stabilize the characteristics of the nanoparticles. Detailed information regarding the chemistry of iron-oxide nanoparticles, common synthesis methods, and some of the factors that affect nanoparticle growth and synthesis have been investigated. The heating ability of the nanoparticles under an oscillating magnetic field (OMF) and the size distribution of the particles under a transmission electron microscope (TEM) are shown.
520 $a The second part of the research focuses on selectively killing the RAW 264.7 macrophages which have internalized the synthesized nanoparticles in order to prove the biocompatibility and effectiveness of the nanoparticles. The particles' effect on the cells, the mechanism of killing, and the effectiveness of nanoparticles coated with colloidal gold and bovine serum albumin are investigated.
520 $a The last part of the research focuses on effectively labeling the mESCs with a specific antibody conjugated to cFeAu nanoparticles that has an affinity to stage specific embryonic antigen 1 (SSEA-1). The influence of the OMF and the effects of immunolabeling on cell growth were investigated. The successful conjugation of the nanoparticles onto the cell surface is shown under scanning electron microscope. The damage inflicted by the nanoparticles on the cells and their effects on neighboring cells are discussed. It was shown that the cells labeled by the cFeAu can be separated from the rest of the cells under the OMF. The success of this new approach with no toxic effects on the desired cells is shown.
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