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Development of Nano/Micro Probes for...

Gao, Yang.

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Development of Nano/Micro Probes for Femtoliter Volume and Single Cell Measurements.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Development of Nano/Micro Probes for Femtoliter Volume and Single Cell Measurements./
Author:	Gao, Yang.
Description:	149 p.
Notes:	Source: Dissertation Abstracts International, Volume: 77-10(E), Section: B.
Contained By:	Dissertation Abstracts International77-10B(E).
Subject:	Nanotechnology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10131816
ISBN:	9781339895871

Development of Nano/Micro Probes for Femtoliter Volume and Single Cell Measurements.
Gao, Yang.

Development of Nano/Micro Probes for Femtoliter Volume and Single Cell Measurements. - 149 p.

Source: Dissertation Abstracts International, Volume: 77-10(E), Section: B.

Thesis (Ph.D.)--Drexel University, 2015.

Single cell analysis has recently emerged as an important field of biomedical re- search. It is now clear that heterogeneity of cell metabolism functions in complex biological systems is correlated to changes in biological function and disease processes. A variety of nano/micro probes were developed to enable investigation of cells properties such as membrane stiffness, pH value. However, very few designs were focused on single cell metabolic function studies. There is a critical need for technologies that provide analysis of heterogeneity of cell metabolic functions, especially on metabolism. Nevertheless, the few existing approaches suffer from fundamental defects and need to be improved.

ISBN: 9781339895871Subjects--Topical Terms:

526235
Nanotechnology.

Development of Nano/Micro Probes for Femtoliter Volume and Single Cell Measurements.
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020 $a 9781339895871
035 $a (MiAaPQ)AAI10131816
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100 1 $a Gao, Yang. $3 3180265
245 1 0 $a Development of Nano/Micro Probes for Femtoliter Volume and Single Cell Measurements.
300 $a 149 p.
500 $a Source: Dissertation Abstracts International, Volume: 77-10(E), Section: B.
500 $a Advisers: Adam Fontecchio; Timothy Kurzweg.
502 $a Thesis (Ph.D.)--Drexel University, 2015.
520 $a Single cell analysis has recently emerged as an important field of biomedical re- search. It is now clear that heterogeneity of cell metabolism functions in complex biological systems is correlated to changes in biological function and disease processes. A variety of nano/micro probes were developed to enable investigation of cells properties such as membrane stiffness, pH value. However, very few designs were focused on single cell metabolic function studies. There is a critical need for technologies that provide analysis of heterogeneity of cell metabolic functions, especially on metabolism. Nevertheless, the few existing approaches suffer from fundamental defects and need to be improved.
520 $a This work focused on developing nano/micro probes that are suitable for single cell functionality investigation. Both types of probes are designed to measure cell-to-cell/time-to-time heterogeneity in metabolic functions over a long period of time. Lab-made carbon nanoprobes were developed especially for electro-physiological measurement. The unique structure of the carbon nanoprobes makes them suitable for important intracellular applications like trans-membrane potential measurements and various electrochemical measurement for cell function studies. While it is important of have ability to carry out intracellular measure, there are also occasions where the information of a cell as a whole is collected. One of the most important indicator of a cells metabolic functions is cell respiration rate/oxygen consumption rate. A micro-perfusion based multi-functional single cell sensing probe was the developed to carry out measurements on cell as a whole. Formed by a double-barrel theta pipette, the perfusion flow enables the direct measurement of the metabolic flux for example oxygen consumption rate.
520 $a In conclusion, this work developed nano/micro-probes as novel single cell investigation tools. The data acquired from these tools could provide valuable assistance on applications including cell metabolism studies, cancer diagnoses, and therapy evaluations.
590 $a School code: 0065.
650 4 $a Nanotechnology. $3 526235
650 4 $a Biophysics. $3 518360
650 4 $a Analytical chemistry. $3 3168300
690 $a 0652
690 $a 0786
690 $a 0486
710 2 $a Drexel University. $b Electrical Engineering - College of Engineering. $3 3192805
773 0 $t Dissertation Abstracts International $g 77-10B(E).
790 $a 0065
791 $a Ph.D.
792 $a 2015
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10131816