東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back

Switch To: Labeled | MARC Mode | ISBD

Endothelin-Converting Enzyme-1 Depen...

Johnson, Michael G.

Linked to FindBook

Google Book

Amazon

博客來

Endothelin-Converting Enzyme-1 Dependent Endothelin 1 Signaling in Osteogenesis.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Endothelin-Converting Enzyme-1 Dependent Endothelin 1 Signaling in Osteogenesis./
Author:	Johnson, Michael G.
Description:	107 p.
Notes:	Source: Dissertation Abstracts International, Volume: 77-05(E), Section: B.
Contained By:	Dissertation Abstracts International77-05B(E).
Subject:	Endocrinology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3746011
ISBN:	9781339391281

Endothelin-Converting Enzyme-1 Dependent Endothelin 1 Signaling in Osteogenesis.
Johnson, Michael G.

Endothelin-Converting Enzyme-1 Dependent Endothelin 1 Signaling in Osteogenesis. - 107 p.

Source: Dissertation Abstracts International, Volume: 77-05(E), Section: B.

Thesis (Ph.D.)--The University of Wisconsin - Madison, 2016.

Previously, we identified Ece1, encoding endothelin-converting-enzyme-1 (ECE1), as a positional candidate for a pleiotropic quantitative trait locus affecting femoral size, shape, and biomechanical performance. We bred congenic mice to isolate the smallest possible DNA interval within Bmd7 that affects bone size and strength (3MB) and found that this interval, containing Ece1, affects bone size and strength. To test the hypothesis that endothelin 1 (ET1) signaling influences mineralization of osteoblasts, we exposed immortalized mouse osteoblast-like (TMOb) cells to big ET1 resulting in greater mineralization, increased RUNX2 and collagen production, increased secretion of IGF1 and decreased secretion of SOST and DKK1 than unexposed TMOb cells. Blockade of ET1 signaling in TMOb cells reduced mineralization and decreased IGF1 secretion and increased secretion of SOST and DKK1. In ex vivo bone organ culture on bovine and human trabecular bone cores, we determined the effect of exogenous big ET1 and EDNRA blockade on osteogenesis and response to mechanical load in whole tissue. Addition of exogenous big ET1 increased the percent change of elastic modulus (Eapp) relative to control. In human subjects, blockade of ET1 decreased Eapp in the female subject but not the male. We built Tamoxifen inducible and osteoblast lineage Ece1 conditional knockouts. We hypothesize that bones from Ece1 knockout mice will have smaller and weaker bones. Analysis of these bone is underway.

ISBN: 9781339391281Subjects--Topical Terms:

610914
Endocrinology.

Endothelin-Converting Enzyme-1 Dependent Endothelin 1 Signaling in Osteogenesis.
LDR:02359nmm a2200265 4500 001 2072631
005 20160808081012.5
008 170521s2016 ||||||||||||||||| ||eng d
020 $a 9781339391281
035 $a (MiAaPQ)AAI3746011
035 $a AAI3746011
040 $a MiAaPQ $c MiAaPQ
100 1 $a Johnson, Michael G. $3 3187831
245 1 0 $a Endothelin-Converting Enzyme-1 Dependent Endothelin 1 Signaling in Osteogenesis.
300 $a 107 p.
500 $a Source: Dissertation Abstracts International, Volume: 77-05(E), Section: B.
500 $a Adviser: Marc K. Drezner.
502 $a Thesis (Ph.D.)--The University of Wisconsin - Madison, 2016.
520 $a Previously, we identified Ece1, encoding endothelin-converting-enzyme-1 (ECE1), as a positional candidate for a pleiotropic quantitative trait locus affecting femoral size, shape, and biomechanical performance. We bred congenic mice to isolate the smallest possible DNA interval within Bmd7 that affects bone size and strength (3MB) and found that this interval, containing Ece1, affects bone size and strength. To test the hypothesis that endothelin 1 (ET1) signaling influences mineralization of osteoblasts, we exposed immortalized mouse osteoblast-like (TMOb) cells to big ET1 resulting in greater mineralization, increased RUNX2 and collagen production, increased secretion of IGF1 and decreased secretion of SOST and DKK1 than unexposed TMOb cells. Blockade of ET1 signaling in TMOb cells reduced mineralization and decreased IGF1 secretion and increased secretion of SOST and DKK1. In ex vivo bone organ culture on bovine and human trabecular bone cores, we determined the effect of exogenous big ET1 and EDNRA blockade on osteogenesis and response to mechanical load in whole tissue. Addition of exogenous big ET1 increased the percent change of elastic modulus (Eapp) relative to control. In human subjects, blockade of ET1 decreased Eapp in the female subject but not the male. We built Tamoxifen inducible and osteoblast lineage Ece1 conditional knockouts. We hypothesize that bones from Ece1 knockout mice will have smaller and weaker bones. Analysis of these bone is underway.
590 $a School code: 0262.
650 4 $a Endocrinology. $3 610914
690 $a 0409
710 2 $a The University of Wisconsin - Madison. $b Endocrinol-Reprod Physiol. $3 3187832
773 0 $t Dissertation Abstracts International $g 77-05B(E).
790 $a 0262
791 $a Ph.D.
792 $a 2016
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3746011