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Detoxification of Lignocellulose-der...

Zhang, Yan.

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Detoxification of Lignocellulose-derived Microbial Inhibitory Compounds by Clostridium beijerinckii NCIMB 8052 during Acetone-Butanol-Ethanol Fermentation.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Detoxification of Lignocellulose-derived Microbial Inhibitory Compounds by Clostridium beijerinckii NCIMB 8052 during Acetone-Butanol-Ethanol Fermentation./
作者:	Zhang, Yan.
面頁冊數:	302 p.
附註:	Source: Dissertation Abstracts International, Volume: 76-05(E), Section: B.
Contained By:	Dissertation Abstracts International76-05B(E).
標題:	Agricultural engineering. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3673580
ISBN:	9781321512151

Detoxification of Lignocellulose-derived Microbial Inhibitory Compounds by Clostridium beijerinckii NCIMB 8052 during Acetone-Butanol-Ethanol Fermentation.
Zhang, Yan.

Detoxification of Lignocellulose-derived Microbial Inhibitory Compounds by Clostridium beijerinckii NCIMB 8052 during Acetone-Butanol-Ethanol Fermentation. - 302 p.

Source: Dissertation Abstracts International, Volume: 76-05(E), Section: B.

Thesis (Ph.D.)--The Ohio State University, 2013.

Pretreatment and hydrolysis of lignocellulosic biomass to fermentable sugars generate a complex mixture of microbial inhibitors such as furan aldehydes (e.g., furfural), which at sublethal concentration in the fermentation medium can be tolerated or detoxified by acetone butanol ethanol (ABE)-producing Clostridium beijerinckii NCIMB 8052. The response of C. beijerinckii to furfural at the molecular level, however, has not been directly studied. Therefore, this study was to elucidate mechanism employed by C. beijerinckii to detoxify lignocellulose-derived microbial inhibitors and use this information to develop inhibitor-tolerant C. beijerinckii .

ISBN: 9781321512151Subjects--Topical Terms:

3168406
Agricultural engineering.

Detoxification of Lignocellulose-derived Microbial Inhibitory Compounds by Clostridium beijerinckii NCIMB 8052 during Acetone-Butanol-Ethanol Fermentation.
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100 1 $a Zhang, Yan. $3 1035418
245 1 0 $a Detoxification of Lignocellulose-derived Microbial Inhibitory Compounds by Clostridium beijerinckii NCIMB 8052 during Acetone-Butanol-Ethanol Fermentation.
300 $a 302 p.
500 $a Source: Dissertation Abstracts International, Volume: 76-05(E), Section: B.
500 $a Adviser: Thaddeus Ezeji.
502 $a Thesis (Ph.D.)--The Ohio State University, 2013.
520 $a Pretreatment and hydrolysis of lignocellulosic biomass to fermentable sugars generate a complex mixture of microbial inhibitors such as furan aldehydes (e.g., furfural), which at sublethal concentration in the fermentation medium can be tolerated or detoxified by acetone butanol ethanol (ABE)-producing Clostridium beijerinckii NCIMB 8052. The response of C. beijerinckii to furfural at the molecular level, however, has not been directly studied. Therefore, this study was to elucidate mechanism employed by C. beijerinckii to detoxify lignocellulose-derived microbial inhibitors and use this information to develop inhibitor-tolerant C. beijerinckii .
520 $a Towards the long-term goal of developing inhibitor-tolerant Clostridium strains, the first objective was to evaluate ABE fermentation by C. beijerinckii using different proportions of Miscanthus giganteus hydrolysates as carbon source. Compared to the growth of C. beijerinckii in control medium, C. beijerinckii experienced different degrees of inhibition. The degree of inhibition was dose-dependent, and C. beijerinckii did not grow in P2 medium with greater than 25% (v/v) Miscanthus giganteus hydrolysates. To improve tolerance of C. beijerinckii to inhibitors, supplementation of P2 medium with undiluted (100%) Miscanthus giganteus hydrolysates with 4 g/L CaCO3 resulted in successful growth of and ABE production by C. beijerinckii. Spectrophotometric and HPLC analyses revealed that C. beijerinckii transformed lignocellulose-derived furan aldehydes such as furfural and hydroxymethylfurfural to furfuryl alcohol and 2, 5-bishydroxymethylfuran, respectively and at a rate of 0.15 and 0.08 g/L/h, respectively.
520 $a The next study aimed to compare differential gene expressions between C. beijerinckii cultures grown in P2 medium supplemented with and without furfural during acidogenic and solventogenic growth phases. The genomic microarray was used to comprehensively evaluate the inhibitory effects of furfural on C. beijerinckii, and potential adaptation mechanisms to furfural stress. Functional gene group analysis showed that increased expression of genes related to redox balancing may be responsible for the reduction of toxic effects of furfural and alleviation of furfural induced oxidative stress in C. beijerinckii during acidogenic growth phase. However, ABE accumulation, redox balance perturbations, and repression of phosphotransferase system may have caused the termination of the growth of C. beijerinckii following furfural challenge at the solventogenic growth phase.
520 $a The last objective was to accelerate biotransformation of furfural to furfuryl alcohol by overexpression of furfural-reducting enzymes in C. beijerinckii. Based on results obtained from the transcriptomic analysis of C. beijerinckii, two candidate genes, aldo/keto reductase (AKR) and short-chain dehydrogenase/reductase (SDR) encoded by Cbei_3974 and Cbei_3904 respectively, were selected, cloned and expressed in Escherichia coli to generate polyhistidine-tagged proteins and confirm the role of these enzymes in furfural reduction. Those (His)6-tagged proteins were purified by immobilized metal affinity chromatography. AKR and SDR reduced furfural to furfuryl alcohol using NADPH as cofactor, and they showed catalytic activities over a broad range of temperature, pH, and substrate specificity. Subsequently, AKR and SDR were overexpressed in C. beijerinckii, which resulted in the development of inhibitor-tolerant strains, C. beijerinckii AKR+ and C. beijerinckii SDR+. This integrated study enhanced our understanding of inhibitory effects of lignocellulose-derived aldehydes, and discussed poteintial strategies to engineer clostridia with high tolerance to lignocellulose hydrolysates.
590 $a School code: 0168.
650 4 $a Agricultural engineering. $3 3168406
650 4 $a Alternative Energy. $3 1035473
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710 2 $a The Ohio State University. $b Animal Sciences. $3 3186497
773 0 $t Dissertation Abstracts International $g 76-05B(E).
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791 $a Ph.D.
792 $a 2013
793 $a English
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