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Detection methods and intestinal adh...

Stromberg, Zachary R.

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Detection methods and intestinal adherence of non-O157 shiga toxin-producing Escherichia coli.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Detection methods and intestinal adherence of non-O157 shiga toxin-producing Escherichia coli./
Author:	Stromberg, Zachary R.
Description:	266 p.
Notes:	Source: Dissertation Abstracts International, Volume: 77-04(E), Section: B.
Contained By:	Dissertation Abstracts International77-04B(E).
Subject:	Veterinary science. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3738359
ISBN:	9781339286280

Detection methods and intestinal adherence of non-O157 shiga toxin-producing Escherichia coli.
Stromberg, Zachary R.

Detection methods and intestinal adherence of non-O157 shiga toxin-producing Escherichia coli. - 266 p.

Source: Dissertation Abstracts International, Volume: 77-04(E), Section: B.

Thesis (Ph.D.)--The University of Nebraska - Lincoln, 2015.

DETECTION METHODS AND INTESTINAL ADHERENCE OF NON-O157 SHIGA TOXIN-PRODUCING ESCHERICHIA COLI.

ISBN: 9781339286280Subjects--Topical Terms:

3172798
Veterinary science.

Detection methods and intestinal adherence of non-O157 shiga toxin-producing Escherichia coli.
LDR:03505nmm a2200325 4500 001 2068138
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020 $a 9781339286280
035 $a (MiAaPQ)AAI3738359
035 $a AAI3738359
040 $a MiAaPQ $c MiAaPQ
100 1 $a Stromberg, Zachary R. $3 3183048
245 1 0 $a Detection methods and intestinal adherence of non-O157 shiga toxin-producing Escherichia coli.
300 $a 266 p.
500 $a Source: Dissertation Abstracts International, Volume: 77-04(E), Section: B.
500 $a Adviser: Rodney A. Moxley.
502 $a Thesis (Ph.D.)--The University of Nebraska - Lincoln, 2015.
520 $a DETECTION METHODS AND INTESTINAL ADHERENCE OF NON-O157 SHIGA TOXIN-PRODUCING ESCHERICHIA COLI.
520 $a Zachary R. Stromberg, Ph.D.
520 $a University of Nebraska, 2015.
520 $a Advisor: Rodney A. Moxley.
520 $a Shiga toxin-producing Escherichia coli (STEC) are enteric pathogens of humans. Cattle serve as a reservoir and harbor STEC in their intestines. Intimin-positive STEC are referred to as enterohemorrhagic E.coli (EHEC). Seven serogroups (O26, O45, O103, O111, O121, O145, and O157) account for the majority of illness due to STEC and are hereafter referred to as STEC/EHEC-7. To improve detection, enrichment broths were compared for supporting growth of STEC-7 and STEC O104:H4 (STEC-8). In pure culture, STEC enriched in trypticase soy broth (TSB) had significantly greater growth compared to TSB containing antimicrobials. In fecal samples, E. coli broth enrichment yielded growth of STEC-8 that was significantly greater than in TSB. Optimized culture conditions allow for greater detection of EHEC-7 in cattle. To determine the prevalence of EHEC-7 in feedlot cattle, culture-based methods and molecular screening assays were used. In 576 feedlot cattle, EHEC-7 prevalence in hide samples as detected by NeoSEEK (NS) was 80.7% compared to 1.2% by culture. The prevalence of EHEC-7 on carcasses was 6.0% detected by NS. Additionally, EHEC-7 prevalence was determined in 100 culled dairy cows. The EHEC-7 prevalence in feces, hides, and carcasses, respectively, was 6.5%, 15.6%, and 1.0% by culture, and 25.9%, 64.9%, and 7.0% by NS. These studies provide evidence that EHEC are ubiquitous on cattle hides and to a lesser extent feces and carcasses. Given the discordant results, continued improvement in EHEC-7 detection methods is needed. Comparison studies were performed using CHROMagar STEC, Posse differential agar (Posse), Posse with modified antimicrobials, STEC heart infusion washed blood agar with mitomycin C (SHIBAM), and SHIBAM with modified antimicrobials. CHROMagar STEC performed better than Posse or SHIBAM for detection of EHEC-7 in cattle feces, but modifications of the antimicrobials in the latter two media resulted in significant improvements. STEC attachment facilitates colonization of the intestine. All STEC-8 strains tested adhered to bovine colonic explants and Caco-2 cells. One strain invaded both bovine colonic epithelial cells and Caco-2 cells. STEC O104:H4 had significantly higher levels of adherence on Caco-2 cells compared to most STEC. Interventions which block adherence may be effective for pre-harvest control.
590 $a School code: 0138.
650 4 $a Veterinary science. $3 3172798
650 4 $a Microbiology. $3 536250
690 $a 0778
690 $a 0410
710 2 $a The University of Nebraska - Lincoln. $b Integrative Biomedical Sciences. $3 3183049
773 0 $t Dissertation Abstracts International $g 77-04B(E).
790 $a 0138
791 $a Ph.D.
792 $a 2015
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3738359