東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back

Switch To: Labeled | MARC Mode | ISBD

Extensin Peroxidase Identification a...

Dong, Wen.

Linked to FindBook

Google Book

Amazon

博客來

Extensin Peroxidase Identification and Characterization in Solanum lycopersicum.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Extensin Peroxidase Identification and Characterization in Solanum lycopersicum./
Author:	Dong, Wen.
Description:	205 p.
Notes:	Source: Dissertation Abstracts International, Volume: 77-02(E), Section: B.
Contained By:	Dissertation Abstracts International77-02B(E).
Subject:	Biochemistry. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3729408
ISBN:	9781339152622

Extensin Peroxidase Identification and Characterization in Solanum lycopersicum.
Dong, Wen.

Extensin Peroxidase Identification and Characterization in Solanum lycopersicum. - 205 p.

Source: Dissertation Abstracts International, Volume: 77-02(E), Section: B.

Thesis (Ph.D.)--Ohio University, 2015.

The regulation of plant cell growth and defense involves the insolubilization of hydroxyproline-rich glycoproteins (HRGPs), such as extensin, in the primary cell wall. In tomato (Solanum lycopersicum ), insolublization occurs by the formation of tyrosylcrosslinks catalyzed specifically by the pI 4.6 extensin peroxidase (EP). To date, neither the gene encoding EP nor the protein itself has been identified.

ISBN: 9781339152622Subjects--Topical Terms:

518028
Biochemistry.

Extensin Peroxidase Identification and Characterization in Solanum lycopersicum.
LDR:02531nmm a2200301 4500 001 2067054
005 20160321080125.5
008 170521s2015 ||||||||||||||||| ||eng d
020 $a 9781339152622
035 $a (MiAaPQ)AAI3729408
035 $a AAI3729408
040 $a MiAaPQ $c MiAaPQ
100 1 $a Dong, Wen. $3 3181910
245 1 0 $a Extensin Peroxidase Identification and Characterization in Solanum lycopersicum.
300 $a 205 p.
500 $a Source: Dissertation Abstracts International, Volume: 77-02(E), Section: B.
500 $a Advisers: Marcia J. Kieliszewski; Michael A. Held.
502 $a Thesis (Ph.D.)--Ohio University, 2015.
520 $a The regulation of plant cell growth and defense involves the insolubilization of hydroxyproline-rich glycoproteins (HRGPs), such as extensin, in the primary cell wall. In tomato (Solanum lycopersicum ), insolublization occurs by the formation of tyrosylcrosslinks catalyzed specifically by the pI 4.6 extensin peroxidase (EP). To date, neither the gene encoding EP nor the protein itself has been identified.
520 $a Here, tomato EP candidates were identified using both proteomic and bioinformatic approaches. Bioinformatic screening of the tomato genome yielded eight EP gene candidates, which encoded a putative signal sequence and a protein with a predicted pI near 4.6. Biochemical fractionation of tomato culture media followed by proteomic detection further refined our list of EP candidates to three, with the lead candidate designated a "CG5". The CG5 open-reading frame from a tomato cDNA was then cloned into a bacterial expression vector to test for EP crosslinking activity.
520 $a CG5 was expressed in E. coli, fractionated from inclusion bodies, and folded in vitro. Peroxidase activity of CG5 was assayed and quantified by the ABTS (2,2'- zinobis(3-ethylbenzothiazoline-6-sulphonic acid)) assay. Subsequent extensin crosslinking assays showed that CG5 can covalently crosslink authentic tomato P1 extensin and P3-type extensin analogs in vitro supporting the hypothesis that CG5 encodes a tomato EP. Recombinant CG5 was then purified for enzyme kinetics study which gave out a Km of 0.105 mM. A computational 3D model was generated for CG5 with highlighted active site and predicted heme group binding.
590 $a School code: 0167.
650 4 $a Biochemistry. $3 518028
650 4 $a Botany. $3 516217
690 $a 0487
690 $a 0309
710 2 $a Ohio University. $b Chemistry and Biochemistry (Arts and Sciences). $3 3181911
773 0 $t Dissertation Abstracts International $g 77-02B(E).
790 $a 0167
791 $a Ph.D.
792 $a 2015
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3729408