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Investigation of microbial aspects r...

Howe, Kevin Matthew.

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Investigation of microbial aspects related to Salmonella as a food pathogen: bioluminescent reporting system and mechanisms for host invasion.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Investigation of microbial aspects related to Salmonella as a food pathogen: bioluminescent reporting system and mechanisms for host invasion./
Author:	Howe, Kevin Matthew.
Description:	119 p.
Notes:	Source: Dissertation Abstracts International, Volume: 76-12(E), Section: B.
Contained By:	Dissertation Abstracts International76-12B(E).
Subject:	Microbiology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3716700
ISBN:	9781321958560

Investigation of microbial aspects related to Salmonella as a food pathogen: bioluminescent reporting system and mechanisms for host invasion.
Howe, Kevin Matthew.

Investigation of microbial aspects related to Salmonella as a food pathogen: bioluminescent reporting system and mechanisms for host invasion. - 119 p.

Source: Dissertation Abstracts International, Volume: 76-12(E), Section: B.

Thesis (Ph.D.)--Mississippi State University, 2015.

Salmonella can reside in healthy animals without the manifestation of any adverse effects on the carrier. If raw products of animal origin are not handled properly during processing or cooked to a proper temperature during preparation, salmonellosis can occur. In this research, microbial aspects related to Salmonella as a food pathogen are investigated. A bioluminescent reporting system was developed for Salmonella to monitor the attachment and growth of the pathogen on food products. Twelve and eleven Salmonella strains from the broiler production continuum were tagged with bioluminescence by plasmid and integration of the lux operon into the chromosome, respectively. To assess the usefulness of bioluminescent Salmonella strains in food safety studies, an attachment model using chicken skin was developed. Variables including washing and temperature were tested in the attachment model to determine the effects on attachment of Salmonella strains to chicken skin, a characteristic that enhances persistence during processing. Additionally, the invasion process for two serovars of Salmonella with differing host tropism was examined with emphasis on the initial establishment of the bacterium in the host. The major facilitator for invasion, type III secretion system, was inactivated through deletion mutation to evaluate invasion of human epithelial cell line by additional means. The difference in host tropism between the two subspecies of Salmonella was also taken into account when evaluating invasion. Results showed that invasion of human epithelial cells can be initiated despite inactivation of the type III secretion system. A serovar of Salmonella that is not typically associated with human illness was also shown to initiate invasion of human epithelial cells, a result that carries public health implication as this serovar has recently been shown to be multi-drug resistant.

ISBN: 9781321958560Subjects--Topical Terms:

536250
Microbiology.

Investigation of microbial aspects related to Salmonella as a food pathogen: bioluminescent reporting system and mechanisms for host invasion.
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100 1 $a Howe, Kevin Matthew. $3 3181876
245 1 0 $a Investigation of microbial aspects related to Salmonella as a food pathogen: bioluminescent reporting system and mechanisms for host invasion.
300 $a 119 p.
500 $a Source: Dissertation Abstracts International, Volume: 76-12(E), Section: B.
500 $a Adviser: Richard H. Bailey.
502 $a Thesis (Ph.D.)--Mississippi State University, 2015.
520 $a Salmonella can reside in healthy animals without the manifestation of any adverse effects on the carrier. If raw products of animal origin are not handled properly during processing or cooked to a proper temperature during preparation, salmonellosis can occur. In this research, microbial aspects related to Salmonella as a food pathogen are investigated. A bioluminescent reporting system was developed for Salmonella to monitor the attachment and growth of the pathogen on food products. Twelve and eleven Salmonella strains from the broiler production continuum were tagged with bioluminescence by plasmid and integration of the lux operon into the chromosome, respectively. To assess the usefulness of bioluminescent Salmonella strains in food safety studies, an attachment model using chicken skin was developed. Variables including washing and temperature were tested in the attachment model to determine the effects on attachment of Salmonella strains to chicken skin, a characteristic that enhances persistence during processing. Additionally, the invasion process for two serovars of Salmonella with differing host tropism was examined with emphasis on the initial establishment of the bacterium in the host. The major facilitator for invasion, type III secretion system, was inactivated through deletion mutation to evaluate invasion of human epithelial cell line by additional means. The difference in host tropism between the two subspecies of Salmonella was also taken into account when evaluating invasion. Results showed that invasion of human epithelial cells can be initiated despite inactivation of the type III secretion system. A serovar of Salmonella that is not typically associated with human illness was also shown to initiate invasion of human epithelial cells, a result that carries public health implication as this serovar has recently been shown to be multi-drug resistant.
590 $a School code: 0132.
650 4 $a Microbiology. $3 536250
650 4 $a Molecular biology. $3 517296
650 4 $a Veterinary science. $3 3172798
690 $a 0410
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710 2 $a Mississippi State University. $b Veterinary Medicine. $3 1263176
773 0 $t Dissertation Abstracts International $g 76-12B(E).
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791 $a Ph.D.
792 $a 2015
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3716700