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Metabolomic Profiling of Natural Pro...

Kowalsick, Amanda Leigh.

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Metabolomic Profiling of Natural Products.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Metabolomic Profiling of Natural Products./
Author:	Kowalsick, Amanda Leigh.
Description:	145 p.
Notes:	Source: Dissertation Abstracts International, Volume: 76-10(E), Section: B.
Contained By:	Dissertation Abstracts International76-10B(E).
Subject:	Analytical chemistry. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3704590
ISBN:	9781321771060

Metabolomic Profiling of Natural Products.
Kowalsick, Amanda Leigh.

Metabolomic Profiling of Natural Products. - 145 p.

Source: Dissertation Abstracts International, Volume: 76-10(E), Section: B.

Thesis (Ph.D.)--Tufts University, 2015.

Plant-based diets have been used for therapeutic purposes, including the prevention and treatment of disease dating back to early times. The upsurge in consumer demand for health-enhancing, biologically active products can be attributed to the growing awareness that diets rich in plant-based materials may help to combat chronic diseases. Plants produce a diverse array of secondary metabolites which assist in the protection and reproduction of the plant. Variation in secondary metabolites produced by plants in response to abiotic and biotic stresses is a key factor that dictates the nutritional, functional and sensory quality of plant-based products. The chemical content of plant volatile extract varies with regards to plant species, geographical origin, cultivar, plant organ, maturity, and environmental factors, making identification of individual constituents difficult.

ISBN: 9781321771060Subjects--Topical Terms:

3168300
Analytical chemistry.

Metabolomic Profiling of Natural Products.
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100 1 $a Kowalsick, Amanda Leigh. $3 3181841
245 1 0 $a Metabolomic Profiling of Natural Products.
300 $a 145 p.
500 $a Source: Dissertation Abstracts International, Volume: 76-10(E), Section: B.
500 $a Adviser: Albert Robbat, Jr.
502 $a Thesis (Ph.D.)--Tufts University, 2015.
520 $a Plant-based diets have been used for therapeutic purposes, including the prevention and treatment of disease dating back to early times. The upsurge in consumer demand for health-enhancing, biologically active products can be attributed to the growing awareness that diets rich in plant-based materials may help to combat chronic diseases. Plants produce a diverse array of secondary metabolites which assist in the protection and reproduction of the plant. Variation in secondary metabolites produced by plants in response to abiotic and biotic stresses is a key factor that dictates the nutritional, functional and sensory quality of plant-based products. The chemical content of plant volatile extract varies with regards to plant species, geographical origin, cultivar, plant organ, maturity, and environmental factors, making identification of individual constituents difficult.
520 $a The complex nature of plant-based products makes it difficult to identify all of the constituents by gas chromatography/mass spectrometry (GC/MS) alone. The work described herein employs automated sequential, multidimensional gas chromatography/mass spectrometry (GC-GC/MS) to obtain matrix-specific, retention time/index and mass spectrometry libraries for natural products. Once targeted metabolite libraries are produced, spectral deconvolution of GC/MS data provides a reliable, unambiguous means to identify constituents in various natural products. Specifically, juniper berry essential oil constituents were tracked from starting materials through the manufacturing process to final gin product as a means to determine product and manufacturing quality. Differences in gin products from several manufacturers were observed based on juniper berry content. Matrix-specific citrus metabolites were used to identify individual oil content in citrus oil mixtures and gin products to determine product authenticity. Finally, metabolites were used to track seasonal variations in teas harvested before and after the onset of the East Asian Monsoon in SW China. Volatile extracts were prepared using two different sample preparation techniques to produce a more comprehensive investigation into the effects extreme weather events have on plant physiology. Striking differences in concentration were observed within and among chemical families in as little as five-days after the monsoon rains began.
590 $a School code: 0234.
650 4 $a Analytical chemistry. $3 3168300
650 4 $a Plant sciences. $3 3173832
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710 2 $a Tufts University. $b Chemistry. $3 1058685
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791 $a Ph.D.
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793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3704590