東華大學圖書館 |

語系: 繁體中文

說明(常見問題)

回圖書館首頁

手機版館藏查詢

登入

回首頁

切換: 標籤 | MARC模式 | ISBD

Turkey arthritis reovirus: pathogene...

Sharaf Eldin, Tamer.

FindBook

Google Book

Amazon

博客來

Turkey arthritis reovirus: pathogenesis and immune response.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Turkey arthritis reovirus: pathogenesis and immune response./
作者:	Sharaf Eldin, Tamer.
面頁冊數:	172 p.
附註:	Source: Dissertation Abstracts International, Volume: 76-08(E), Section: B.
Contained By:	Dissertation Abstracts International76-08B(E).
標題:	Veterinary science. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3686989
ISBN:	9781321639964

Turkey arthritis reovirus: pathogenesis and immune response.
Sharaf Eldin, Tamer.

Turkey arthritis reovirus: pathogenesis and immune response. - 172 p.

Source: Dissertation Abstracts International, Volume: 76-08(E), Section: B.

Thesis (Ph.D.)--University of Minnesota, 2015.

In 2011, turkey reoviruses were isolated from tendons and synovial fluids of >15-week-old lame turkeys displaying swollen joints and occasionally ruptured leg tendons in Midwest, USA. These reoviruses were tentatively called turkey arthritis reoviruses (TARV) to differentiate them from reoviruses isolated from intestinal contents and feces of turkeys namely turkey enteric reoviruses (TERV). TARV were found to be genetically distinct from chicken arthritis reoviruses (CARV). Five experiments were conducted to test the pathogenicity of TARV in turkeys and in chickens and to compare it with that of TERV and CARV. Additionally, this work investigated the virus pathogenesis and cytokine immune responses. TARV showed unique capability to induce significantly higher tenosynovitis scores in turkeys as compared with TERV and CARV which induced minimal scores. Clinical lameness was first displayed at 8 weeks of age in TARV-inoculated turkeys at 1 week of age. Lameness in infected group reached approximately 50% at 16 weeks of age. TARV did not induce any lesions in chickens via intratracheal or oral route. TARV inoculation via footpad route induced tenosynovitis in chickens at 2 and 3 weeks PI with no clinical lameness. In pathogenesis study, TARV displayed the greatest replication in intestines and bursa of Fabricius than in leg tendons of turkeys. Viral infection mediated effective antiviral cytokines immune response that limited virus replication in the intestines. Furthermore, viral infection mediated a significantly elevated T helper-1(Th1) cytokine response in intestines and tendons and minimal Th2 and Th17 cytokine response during the early stage (2 weeks) of infection. This work established an experimental model to study TARV which provides early end points that are indicative of disease pathogenicity. Additionally this work developed a new grading system for histologic tenosynovitis which can be used in a wide variety of experimental models. For lameness evaluation in turkeys, this work developed a grading system for gait scores. In summary, this work showed unique pathogenicity of the newly isolated TARV and added significant knowledge to TARV pathogenesis and immune response using the newly established reproducible experimental model and the newly developed grading systems for evaluation of tenosynovitis and clinical lameness.

ISBN: 9781321639964Subjects--Topical Terms:

3172798
Veterinary science.

Turkey arthritis reovirus: pathogenesis and immune response.
LDR:03223nmm a2200265 4500 001 2066958
005 20160321080102.5
008 170521s2015 ||||||||||||||||| ||eng d
020 $a 9781321639964
035 $a (MiAaPQ)AAI3686989
035 $a AAI3686989
040 $a MiAaPQ $c MiAaPQ
100 1 $a Sharaf Eldin, Tamer. $3 3181813
245 1 0 $a Turkey arthritis reovirus: pathogenesis and immune response.
300 $a 172 p.
500 $a Source: Dissertation Abstracts International, Volume: 76-08(E), Section: B.
500 $a Advisers: Robert E. Porter; Sagar M. Goyal.
502 $a Thesis (Ph.D.)--University of Minnesota, 2015.
520 $a In 2011, turkey reoviruses were isolated from tendons and synovial fluids of >15-week-old lame turkeys displaying swollen joints and occasionally ruptured leg tendons in Midwest, USA. These reoviruses were tentatively called turkey arthritis reoviruses (TARV) to differentiate them from reoviruses isolated from intestinal contents and feces of turkeys namely turkey enteric reoviruses (TERV). TARV were found to be genetically distinct from chicken arthritis reoviruses (CARV). Five experiments were conducted to test the pathogenicity of TARV in turkeys and in chickens and to compare it with that of TERV and CARV. Additionally, this work investigated the virus pathogenesis and cytokine immune responses. TARV showed unique capability to induce significantly higher tenosynovitis scores in turkeys as compared with TERV and CARV which induced minimal scores. Clinical lameness was first displayed at 8 weeks of age in TARV-inoculated turkeys at 1 week of age. Lameness in infected group reached approximately 50% at 16 weeks of age. TARV did not induce any lesions in chickens via intratracheal or oral route. TARV inoculation via footpad route induced tenosynovitis in chickens at 2 and 3 weeks PI with no clinical lameness. In pathogenesis study, TARV displayed the greatest replication in intestines and bursa of Fabricius than in leg tendons of turkeys. Viral infection mediated effective antiviral cytokines immune response that limited virus replication in the intestines. Furthermore, viral infection mediated a significantly elevated T helper-1(Th1) cytokine response in intestines and tendons and minimal Th2 and Th17 cytokine response during the early stage (2 weeks) of infection. This work established an experimental model to study TARV which provides early end points that are indicative of disease pathogenicity. Additionally this work developed a new grading system for histologic tenosynovitis which can be used in a wide variety of experimental models. For lameness evaluation in turkeys, this work developed a grading system for gait scores. In summary, this work showed unique pathogenicity of the newly isolated TARV and added significant knowledge to TARV pathogenesis and immune response using the newly established reproducible experimental model and the newly developed grading systems for evaluation of tenosynovitis and clinical lameness.
590 $a School code: 0130.
650 4 $a Veterinary science. $3 3172798
690 $a 0778
710 2 $a University of Minnesota. $b Veterinary Medicine. $3 1682756
773 0 $t Dissertation Abstracts International $g 76-08B(E).
790 $a 0130
791 $a Ph.D.
792 $a 2015
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3686989