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An in vivo system for the identifica...

Lee, Richard Heungki.

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An in vivo system for the identification of ligands for peroxisome proliferatoractivated receptors.

Record Type:	Electronic resources : Monograph/item
Title/Author:	An in vivo system for the identification of ligands for peroxisome proliferatoractivated receptors./
Author:	Lee, Richard Heungki.
Description:	86 p.
Notes:	Source: Masters Abstracts International, Volume: 55-02.
Contained By:	Masters Abstracts International55-02(E).
Subject:	Molecular biology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1604421
ISBN:	9781339269603

An in vivo system for the identification of ligands for peroxisome proliferatoractivated receptors.
Lee, Richard Heungki.

An in vivo system for the identification of ligands for peroxisome proliferatoractivated receptors. - 86 p.

Source: Masters Abstracts International, Volume: 55-02.

Thesis (M.Sc.)--University of Toronto (Canada), 2015.

Peroxisome proliferator-activated receptors (PPARs) are a group of nuclear receptors (NRs) that play major roles in metabolism and development and, when disrupted, lead to disorders such as cancer, diabetes and obesity. Our laboratory has established a transgenic Ligand Trap system in zebrafish for high throughput screening of drugs to identify novel activators of human NRs. We have identified Coenzyme Q10 (CoQ10) as an activator of PPAR-gamma in a presumed tissue specific manner. To demonstrate that CoQ10 directly binds to PPARs, we expressed PPARs in bacteria and zebrafish embryos and treated with CoQ10. Ligand-bound receptors were purified and analyzed by denaturing mass spectrometry. Data suggest that CoQ10 binds to PPAR-alpha and PPAR-gamma, but not to PPAR-beta. Future experiments will focus to confirm direct binding to PPAR subtypes. Once verified, subsequent studies will explore the significance of CoQ10 as a PPAR ligand in the context of transcription machinery complex formation.

ISBN: 9781339269603Subjects--Topical Terms:

517296
Molecular biology.

An in vivo system for the identification of ligands for peroxisome proliferatoractivated receptors.
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245 1 3 $a An in vivo system for the identification of ligands for peroxisome proliferatoractivated receptors.
300 $a 86 p.
500 $a Source: Masters Abstracts International, Volume: 55-02.
500 $a Adviser: Henry Krause.
502 $a Thesis (M.Sc.)--University of Toronto (Canada), 2015.
520 $a Peroxisome proliferator-activated receptors (PPARs) are a group of nuclear receptors (NRs) that play major roles in metabolism and development and, when disrupted, lead to disorders such as cancer, diabetes and obesity. Our laboratory has established a transgenic Ligand Trap system in zebrafish for high throughput screening of drugs to identify novel activators of human NRs. We have identified Coenzyme Q10 (CoQ10) as an activator of PPAR-gamma in a presumed tissue specific manner. To demonstrate that CoQ10 directly binds to PPARs, we expressed PPARs in bacteria and zebrafish embryos and treated with CoQ10. Ligand-bound receptors were purified and analyzed by denaturing mass spectrometry. Data suggest that CoQ10 binds to PPAR-alpha and PPAR-gamma, but not to PPAR-beta. Future experiments will focus to confirm direct binding to PPAR subtypes. Once verified, subsequent studies will explore the significance of CoQ10 as a PPAR ligand in the context of transcription machinery complex formation.
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