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Functional characterization of Dennd...

Qu, Enping.

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Functional characterization of Dennd5a/Rab6ip1 in early embryonic development.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Functional characterization of Dennd5a/Rab6ip1 in early embryonic development./
作者:	Qu, Enping.
面頁冊數:	86 p.
附註:	Source: Masters Abstracts International, Volume: 55-01.
Contained By:	Masters Abstracts International55-01(E).
標題:	Cellular biology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1597123
ISBN:	9781321997958

Functional characterization of Dennd5a/Rab6ip1 in early embryonic development.
Qu, Enping.

Functional characterization of Dennd5a/Rab6ip1 in early embryonic development. - 86 p.

Source: Masters Abstracts International, Volume: 55-01.

Thesis (M.S.)--Temple University, 2015.

Denn stands for differentially expressed in normal vs neoplastic cells, Dennd5a or Denn domain containing protein 5a is also named Rab6 interacting protein 1 (Rab6ip1), KIAA1091 (HUGE database). Rab6 is a small GTP-binding protein that involves in intra-Golgi transport. In a yeast two hybrid screen by Bruno Goud group in 1995, they used Rab6 as bait and found several interacting cDNA clones including Rab6 interacting protein 1 or Dennd5a. Several Dennd5a interaction partners have been discovered, Rab6, Rab11 and Sorting Nexin 1, which primarily locate Dennd5a to intracellular transport pathway. In Dr. Habas lab, Dennd5a was identified as a Daam1 interacting protein in a yeast two hybrid screen. Daam1 is a key player in non-canonical Wnt signaling and regulates vertebrate gastrulation and neural tube closure. I confirmed that Daam1 can physically bind to Dennd5a in immunoprecipitation assays using epitope tagged constructs. In the cellular level experiment, Dennd5a can colocalize with Daam1 on/near cell membrane and within punctate structures, however, Wnt5a and Wnt3a stimulations don't appear to affect their subcellular colocalization. In Xenopus in-vivo experiments, knockdown of Dennd5a by morpholino leads to unclosed blastopore and "open-back" defect during embryonic development. Interestingly, overexpression of Dennd5a by microinjection of RNA leads to very similar phenotype.

ISBN: 9781321997958Subjects--Topical Terms:

3172791
Cellular biology.

Functional characterization of Dennd5a/Rab6ip1 in early embryonic development.
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