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Diversity within the genus Thermoana...

Verbeke, Tobin James.

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Diversity within the genus Thermoanaerobacter and its potential implications in lignocellulosic biofuel production through consolidated bioprocessing.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Diversity within the genus Thermoanaerobacter and its potential implications in lignocellulosic biofuel production through consolidated bioprocessing./
作者:	Verbeke, Tobin James.
面頁冊數:	397 p.
附註:	Source: Dissertation Abstracts International, Volume: 75-07(E), Section: B.
Contained By:	Dissertation Abstracts International75-07B(E).
標題:	Microbiology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=NS28287
ISBN:	9780499282873

Diversity within the genus Thermoanaerobacter and its potential implications in lignocellulosic biofuel production through consolidated bioprocessing.
Verbeke, Tobin James.

Diversity within the genus Thermoanaerobacter and its potential implications in lignocellulosic biofuel production through consolidated bioprocessing. - 397 p.

Source: Dissertation Abstracts International, Volume: 75-07(E), Section: B.

Thesis (Ph.D.)--University of Manitoba (Canada), 2014.

This item must not be sold to any third party vendors.

A major obstacle to achieving commercially viable lignocellulosic biofuels through consolidated bioprocessing (CBP) is the lack of "industry-ready" microorganisms. Ideally, a CBP-relevant organism would achieve efficient and complete hydrolysis of lignocellulose, simultaneous utilization of the diverse hydrolysis products and high yields of the desired biofuel. To date, no single microbe has been identified that can perform all of these processes at industrially significant levels.

ISBN: 9780499282873Subjects--Topical Terms:

536250
Microbiology.

Diversity within the genus Thermoanaerobacter and its potential implications in lignocellulosic biofuel production through consolidated bioprocessing.
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245 1 0 $a Diversity within the genus Thermoanaerobacter and its potential implications in lignocellulosic biofuel production through consolidated bioprocessing.
300 $a 397 p.
500 $a Source: Dissertation Abstracts International, Volume: 75-07(E), Section: B.
502 $a Thesis (Ph.D.)--University of Manitoba (Canada), 2014.
506 $a This item must not be sold to any third party vendors.
506 $a This item must not be added to any third party search indexes.
520 $a A major obstacle to achieving commercially viable lignocellulosic biofuels through consolidated bioprocessing (CBP) is the lack of "industry-ready" microorganisms. Ideally, a CBP-relevant organism would achieve efficient and complete hydrolysis of lignocellulose, simultaneous utilization of the diverse hydrolysis products and high yields of the desired biofuel. To date, no single microbe has been identified that can perform all of these processes at industrially significant levels.
520 $a As such, thermophilic decaying woodchip compost was investigated as a source of novel lignocellulolytic, biofuel producing bacteria. From a single sample, a collection of physiologically diverse strains were isolated, which displayed differences in substrate utilization and biofuel production capabilities. Molecular characterization of these isolates, and development of a genome relatedness prediction model based on the chaperonin-60 universal target sequence, identified these isolates as strains of Thermoanaerobacter thermohydrosulfuricus. Application of this model to other Thermoanaerobacter spp. further identified that these isolates belong to a divergent and lesser characterized lineage within the genus.
520 $a Based on this, the CBP-potential of a single isolate, T. thermohydrosulfuricus WC1, was selected for further investigation through metabolic, genomic and proteomic analyses. Its ability to grow on polymeric xylan, potentially catalyzed by an endoxylanase found in only a few Thermoanaerobacter strains, distinguishes T. thermohydrosulfuricus WC1 from many other strains within the genus. The simultaneous consumption of two important lignocellulose constituent saccharides, cellobiose and xylose was also observed and represents a desirable phenotype in CBP-relevant organisms.
520 $a However, at elevated sugar concentrations, T. thermohydrosulfuricus WC1 produces principally lactate, rather than the desired biofuel ethanol, as the major endproduct. Proteomic analysis identified that all likely end-product forming proteins were expressed at high levels suggesting that the end-product distribution patterns in T. thermohydrosulfuricus WC1 are likely controlled via metabolite-based regulation or are constrained by metabolic bottlenecks.
520 $a The xylanolytic and simultaneous substrate utilization capabilities of T. thermohydrosulfuricus WC1 identify it as a strain of interest for CBP. However, for its development into an "industry-ready" strain as a co-culture with a cellulolytic microorganism, improved biofuel producing capabilities are needed. The practical implications of CBP-relevant phenotypes in T. thermohydrosulfuricus WC1 in relation to other Thermoanaerobacter spp. will be discussed.
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773 0 $t Dissertation Abstracts International $g 75-07B(E).
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