東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back

Switch To: Labeled | MARC Mode | ISBD

Biochemical Characterization of Extr...

Killens, Rushyannah R.

Linked to FindBook

Google Book

Amazon

博客來

Biochemical Characterization of Extremophile Fatty Acid Metabolism Enzymes for Use in Algal-Based Biofuel Production.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Biochemical Characterization of Extremophile Fatty Acid Metabolism Enzymes for Use in Algal-Based Biofuel Production./
Author:	Killens, Rushyannah R.
Description:	161 p.
Notes:	Source: Dissertation Abstracts International, Volume: 75-10(E), Section: B.
Contained By:	Dissertation Abstracts International75-10B(E).
Subject:	Microbiology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3584323
ISBN:	9781303998348

Biochemical Characterization of Extremophile Fatty Acid Metabolism Enzymes for Use in Algal-Based Biofuel Production.
Killens, Rushyannah R.

Biochemical Characterization of Extremophile Fatty Acid Metabolism Enzymes for Use in Algal-Based Biofuel Production. - 161 p.

Source: Dissertation Abstracts International, Volume: 75-10(E), Section: B.

Thesis (Ph.D.)--North Carolina State University, 2014.

This item must not be sold to any third party vendors.

There is a renewed interest in renewable energy due to concerns over long-term fossil fuel supply, global warming, and global human population growth. Although promising alternative fuel sources have been derived from food crops (first-generation) and lignocellulose biomass (second generation), these feedstocks are not feasible for commercial use due to competition with food supplies and a requirement for technology development (pretreatment of biomass, enzymatic saccharification of the pretreated biomass, etc.) for the affordable conversion of lignocellulose biomass to fuel. For these reasons, there is a push to produce clean and renewable energy derived from algal biomass. The research reported here is focused on the biochemical characterization of fatty acid synthesis enzymes to augment microalgae-biofuel. Among microalgae, the highly productive, halophilic chlorophytes Dunaliella spp. are a rich source of lipids and have strong potential to be an economically viable source for renewable oil production.

ISBN: 9781303998348Subjects--Topical Terms:

536250
Microbiology.

Biochemical Characterization of Extremophile Fatty Acid Metabolism Enzymes for Use in Algal-Based Biofuel Production.
LDR:03731nmm a2200337 4500 001 2062709
005 20151027073805.5
008 170521s2014 ||||||||||||||||| ||eng d
020 $a 9781303998348
035 $a (MiAaPQ)AAI3584323
035 $a AAI3584323
040 $a MiAaPQ $c MiAaPQ
100 1 $a Killens, Rushyannah R. $3 3177125
245 1 0 $a Biochemical Characterization of Extremophile Fatty Acid Metabolism Enzymes for Use in Algal-Based Biofuel Production.
300 $a 161 p.
500 $a Source: Dissertation Abstracts International, Volume: 75-10(E), Section: B.
500 $a Adviser: Amy M. Grunden.
502 $a Thesis (Ph.D.)--North Carolina State University, 2014.
506 $a This item must not be sold to any third party vendors.
506 $a This item must not be added to any third party search indexes.
520 $a There is a renewed interest in renewable energy due to concerns over long-term fossil fuel supply, global warming, and global human population growth. Although promising alternative fuel sources have been derived from food crops (first-generation) and lignocellulose biomass (second generation), these feedstocks are not feasible for commercial use due to competition with food supplies and a requirement for technology development (pretreatment of biomass, enzymatic saccharification of the pretreated biomass, etc.) for the affordable conversion of lignocellulose biomass to fuel. For these reasons, there is a push to produce clean and renewable energy derived from algal biomass. The research reported here is focused on the biochemical characterization of fatty acid synthesis enzymes to augment microalgae-biofuel. Among microalgae, the highly productive, halophilic chlorophytes Dunaliella spp. are a rich source of lipids and have strong potential to be an economically viable source for renewable oil production.
520 $a To modify carbon flux through the fatty acid biosynthesis pathway and capture fatty acids incorporated into triacylglycerides, lipid biosynthesis genes from extremophiles were selected and biochemically characterized to establish their compatibility for functioning in Dunaliella to increase microalgal oil production. To this end, the acetyl-coenzyme A carboxylase (ACCase) from the bacterial halophile Chromohalobacter salexigens BAA-138 was recombinantly expressed in Escherichia coli to provide sufficient enzyme for biochemical characterization. The ACCase enzyme carries out the rate-limiting step during fatty acid synthesis (FAS), and it has been shown that increased ACCase expression in bacteria leads to elevated rates of FA production. Based on its rate-limiting role in the formation of fatty acids, it is proposed that by expressing a bacterial ACCase in microalgae the FAS limiting effects of transcriptional repression and feedback inhibition of the native microalgal ACCase could be mitigated.
520 $a In addition to increasing lipid production in microalgal strains, thermoactive thioesterases were also identified so that during high temperature conversion of lipids to fuel they could release the free fatty acids (FFAs) previously sequestered during algal cell growth as part of triacylglycerides. Therefore, heat stable thioesterases from Metallosphaera sedula DSM5348 and Sulfolobus solfataricus P2 were recombinantly expressed in E. coli to enable their biochemical characterization and evaluation for suitability for use in microalgae for improved biofuel production.
590 $a School code: 0155.
650 4 $a Microbiology. $3 536250
650 4 $a Molecular biology. $3 517296
650 4 $a Alternative Energy. $3 1035473
690 $a 0410
690 $a 0307
690 $a 0363
710 2 $a North Carolina State University. $b Microbiology. $3 2091961
773 0 $t Dissertation Abstracts International $g 75-10B(E).
790 $a 0155
791 $a Ph.D.
792 $a 2014
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3584323