東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back

Switch To: Labeled | MARC Mode | ISBD

Direct Intercellular Exchange throug...

McLean, Peter Foster.

Linked to FindBook

Google Book

Amazon

博客來

Direct Intercellular Exchange through Somatic Ring Canals in Drosophila.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Direct Intercellular Exchange through Somatic Ring Canals in Drosophila./
Author:	McLean, Peter Foster.
Description:	127 p.
Notes:	Source: Dissertation Abstracts International, Volume: 75-09(E), Section: B.
Contained By:	Dissertation Abstracts International75-09B(E).
Subject:	Cellular biology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3580769
ISBN:	9781321054132

Direct Intercellular Exchange through Somatic Ring Canals in Drosophila.
McLean, Peter Foster.

Direct Intercellular Exchange through Somatic Ring Canals in Drosophila. - 127 p.

Source: Dissertation Abstracts International, Volume: 75-09(E), Section: B.

Thesis (Ph.D.)--Yale University, 2014.

This item must not be sold to any third party vendors.

Ring canals are made from arrested cleavage furrows, and provide direct cytoplasmic connections among sibling cells. They are well documented for their participation in Drosophila oogenesis, but little is known about their role in several somatic tissues in which they are also found. Using a variety of genetic tools in live and fixed tissue, I demonstrate that the &sim;250 nm diameter somatic ring canals permit rapid intercellular exchange through somatic ring canals by diffusion. Additionally, intercellular diffusion of protein was observed between cells with highly disparate levels of mRNA transcript, suggesting a possible role for ring canals in smoothing gene expression within a tissue. I also used a novel combination of markers to evaluate the extent of protein movement within mitotic clones and across clone boundaries in ovarian follicle cells and imaginal discs, providing evidence of robust movement of GFP between the two sides of mitotic clones and frequently into non-recombined cells. These data suggest that, depending on the experimental setup and proteins of interest, inter-clonal diffusion of protein may alter the interpretation of clonal data in follicle cells. Our work illustrates the lack of cytoplasmic autonomy in these tissues and suggests a role for somatic ring canals in promoting homogeneous protein expression within the tissue.

ISBN: 9781321054132Subjects--Topical Terms:

3172791
Cellular biology.

Direct Intercellular Exchange through Somatic Ring Canals in Drosophila.
LDR:02399nmm a2200313 4500 001 2059779
005 20150827082954.5
008 170521s2014 ||||||||||||||||| ||eng d
020 $a 9781321054132
035 $a (MiAaPQ)AAI3580769
035 $a AAI3580769
040 $a MiAaPQ $c MiAaPQ
100 1 $a McLean, Peter Foster. $3 3173908
245 1 0 $a Direct Intercellular Exchange through Somatic Ring Canals in Drosophila.
300 $a 127 p.
500 $a Source: Dissertation Abstracts International, Volume: 75-09(E), Section: B.
500 $a Adviser: Lynn Cooley.
502 $a Thesis (Ph.D.)--Yale University, 2014.
506 $a This item must not be sold to any third party vendors.
506 $a This item must not be added to any third party search indexes.
520 $a Ring canals are made from arrested cleavage furrows, and provide direct cytoplasmic connections among sibling cells. They are well documented for their participation in Drosophila oogenesis, but little is known about their role in several somatic tissues in which they are also found. Using a variety of genetic tools in live and fixed tissue, I demonstrate that the &sim;250 nm diameter somatic ring canals permit rapid intercellular exchange through somatic ring canals by diffusion. Additionally, intercellular diffusion of protein was observed between cells with highly disparate levels of mRNA transcript, suggesting a possible role for ring canals in smoothing gene expression within a tissue. I also used a novel combination of markers to evaluate the extent of protein movement within mitotic clones and across clone boundaries in ovarian follicle cells and imaginal discs, providing evidence of robust movement of GFP between the two sides of mitotic clones and frequently into non-recombined cells. These data suggest that, depending on the experimental setup and proteins of interest, inter-clonal diffusion of protein may alter the interpretation of clonal data in follicle cells. Our work illustrates the lack of cytoplasmic autonomy in these tissues and suggests a role for somatic ring canals in promoting homogeneous protein expression within the tissue.
590 $a School code: 0265.
650 4 $a Cellular biology. $3 3172791
650 4 $a Developmental biology. $3 592588
650 4 $a Genetics. $3 530508
690 $a 0379
690 $a 0758
690 $a 0369
710 2 $a Yale University. $3 515640
773 0 $t Dissertation Abstracts International $g 75-09B(E).
790 $a 0265
791 $a Ph.D.
792 $a 2014
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3580769