東華大學圖書館 |

語系: 繁體中文

說明(常見問題)

回圖書館首頁

手機版館藏查詢

登入

回首頁

切換: 標籤 | MARC模式 | ISBD

Identification of Quantitative Trait...

Florez-Palacios, Sandra Liliana.

FindBook

Google Book

Amazon

博客來

Identification of Quantitative Trait Loci / Genes for Low-stachyose and High-sucrose Content in Soybean Seeds.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Identification of Quantitative Trait Loci / Genes for Low-stachyose and High-sucrose Content in Soybean Seeds./
作者:	Florez-Palacios, Sandra Liliana.
面頁冊數:	155 p.
附註:	Source: Dissertation Abstracts International, Volume: 75-11(E), Section: B.
Contained By:	Dissertation Abstracts International75-11B(E).
標題:	Agriculture, Agronomy. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3628518
ISBN:	9781321045178

Identification of Quantitative Trait Loci / Genes for Low-stachyose and High-sucrose Content in Soybean Seeds.
Florez-Palacios, Sandra Liliana.

Identification of Quantitative Trait Loci / Genes for Low-stachyose and High-sucrose Content in Soybean Seeds. - 155 p.

Source: Dissertation Abstracts International, Volume: 75-11(E), Section: B.

Thesis (Ph.D.)--University of Arkansas, 2014.

Seed carbohydrate content is an important aspect in breeding for food-grade soybeans commercialized in the soyfood market. Sucrose and stachyose are the primary carbohydrates in soybean seed. Sucrose affects the quality and taste of various soyfoods such as tofu, soymilk, and natto; however, consumption of soy-based products with high stachyose concentrations can cause diarrhea and flatulence. A mutant line (V99-5089) with high-sucrose and low-stachyose has been identified. The objectives of this research were: 1) to identify sucrose QTLs in a F2-derived mapping population; 2) to investigate the genetic relationship between two low-stachyose sources, V99-5089 and the germplasm line PI200508; and 3) to identify stachyose QTLs in a F2-derived mapping population. An allelism test for low stachyose was performed by using 121 F2-derived lines from the cross PI200508 x V99-5089 grown in three different environments. Carbohydrate content was determined by a high performance liquid chromatogram system, and lines were classified as high- or low- stachyose. Chi-square analysis was performed to test for goodness-of-fit of observed segregation to the expected genetic ratio. Results showed a 9 high-stachyose : 7 low-stachyose ratio, indicating that two independent recessive genes conferred the low-stachyose trait in the two mutant lines. Additionally, gene dosage effect was observed; however, further study is required in order to confirm its presence. The sucrose and stachyose QTL studies were carried out in 92 F2:7 lines derived from the cross V97-3000 x V99-5089. Leaf samples were collected at F2:6 for DNA extraction and subsequent molecular analysis using single nucleotide polymorphism (SNP) markers. Seed from F2:7 lines grown in two locations, each one with two replications, were analyzed for sugar content. Results showed two sucrose QTLs located on chromosomes 6 and 10, accounting for 17 and 11% of the phenotypic variation, respectively. Additionally, two stachyose QTLs were found on chromosome 10 and 11, explaining 11 and 46% of the stachyose variation. Results from this research indicate that the two low-stachyose sources may serve as valuable parents in breeding for low-stachyose soybeans. Additionally, V99-5089 may also provide favorable alleles for breeding high-sucrose varieties. The sucrose and stachyose QTLs identified in these studies are stable across environments and will facilitate the marker-assisted selection for both traits.

ISBN: 9781321045178Subjects--Topical Terms:

1018679
Agriculture, Agronomy.

Identification of Quantitative Trait Loci / Genes for Low-stachyose and High-sucrose Content in Soybean Seeds.
LDR:03399nmm a2200289 4500 001 2055701
005 20150217125038.5
008 170521s2014 ||||||||||||||||| ||eng d
020 $a 9781321045178
035 $a (MiAaPQ)AAI3628518
035 $a AAI3628518
040 $a MiAaPQ $c MiAaPQ
100 1 $a Florez-Palacios, Sandra Liliana. $3 3169387
245 1 0 $a Identification of Quantitative Trait Loci / Genes for Low-stachyose and High-sucrose Content in Soybean Seeds.
300 $a 155 p.
500 $a Source: Dissertation Abstracts International, Volume: 75-11(E), Section: B.
500 $a Adviser: Pengyin Chen.
502 $a Thesis (Ph.D.)--University of Arkansas, 2014.
520 $a Seed carbohydrate content is an important aspect in breeding for food-grade soybeans commercialized in the soyfood market. Sucrose and stachyose are the primary carbohydrates in soybean seed. Sucrose affects the quality and taste of various soyfoods such as tofu, soymilk, and natto; however, consumption of soy-based products with high stachyose concentrations can cause diarrhea and flatulence. A mutant line (V99-5089) with high-sucrose and low-stachyose has been identified. The objectives of this research were: 1) to identify sucrose QTLs in a F2-derived mapping population; 2) to investigate the genetic relationship between two low-stachyose sources, V99-5089 and the germplasm line PI200508; and 3) to identify stachyose QTLs in a F2-derived mapping population. An allelism test for low stachyose was performed by using 121 F2-derived lines from the cross PI200508 x V99-5089 grown in three different environments. Carbohydrate content was determined by a high performance liquid chromatogram system, and lines were classified as high- or low- stachyose. Chi-square analysis was performed to test for goodness-of-fit of observed segregation to the expected genetic ratio. Results showed a 9 high-stachyose : 7 low-stachyose ratio, indicating that two independent recessive genes conferred the low-stachyose trait in the two mutant lines. Additionally, gene dosage effect was observed; however, further study is required in order to confirm its presence. The sucrose and stachyose QTL studies were carried out in 92 F2:7 lines derived from the cross V97-3000 x V99-5089. Leaf samples were collected at F2:6 for DNA extraction and subsequent molecular analysis using single nucleotide polymorphism (SNP) markers. Seed from F2:7 lines grown in two locations, each one with two replications, were analyzed for sugar content. Results showed two sucrose QTLs located on chromosomes 6 and 10, accounting for 17 and 11% of the phenotypic variation, respectively. Additionally, two stachyose QTLs were found on chromosome 10 and 11, explaining 11 and 46% of the stachyose variation. Results from this research indicate that the two low-stachyose sources may serve as valuable parents in breeding for low-stachyose soybeans. Additionally, V99-5089 may also provide favorable alleles for breeding high-sucrose varieties. The sucrose and stachyose QTLs identified in these studies are stable across environments and will facilitate the marker-assisted selection for both traits.
590 $a School code: 0011.
650 4 $a Agriculture, Agronomy. $3 1018679
650 4 $a Agriculture, Plant Culture. $3 1018669
650 4 $a Biology, Genetics. $3 1017730
690 $a 0285
690 $a 0479
690 $a 0369
710 2 $a University of Arkansas. $b Crop, Soil & Environmental Sciences. $3 3169311
773 0 $t Dissertation Abstracts International $g 75-11B(E).
790 $a 0011
791 $a Ph.D.
792 $a 2014
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3628518