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Low-cost methods for molecular chara...

Till, Bradley J.

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Low-cost methods for molecular characterization of mutant plants = tissue desiccation, DNA extraction and mutation discovery: protocols /

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Low-cost methods for molecular characterization of mutant plants/ by Bradley J. Till ... [et al.].
其他題名:	tissue desiccation, DNA extraction and mutation discovery: protocols /
其他作者:	Till, Bradley J.
出版者:	Cham :Springer International Publishing : : 2015.,
面頁冊數:	x, 35 p. :ill. (some col.), digital ;24 cm.
內容註:	Introduction -- Health and Safety Considerations -- Sample Collection and Storage -- Low-Cost DNA Extraction -- PCR Amplification for Low-Cost Mutation Discovery -- Enzymatic Mismatch Cleavage and Agarose Gel Evaluation of Samples -- Alternative Enzymology for Mismatch Cleavage for TILLING and Ecotilling: Extraction of Enzymes form Common Weedy Plants -- Example Data -- Conclusions.
Contained By:	Springer eBooks
標題:	Plant molecular genetics. -
電子資源:	http://dx.doi.org/10.1007/978-3-319-16259-1
ISBN:	9783319162591 (electronic bk.)

Low-cost methods for molecular characterization of mutant plants = tissue desiccation, DNA extraction and mutation discovery: protocols /
Low-cost methods for molecular characterization of mutant plantstissue desiccation, DNA extraction and mutation discovery: protocols /[electronic resource] :by Bradley J. Till ... [et al.]. - Cham :Springer International Publishing :2015. - x, 35 p. :ill. (some col.), digital ;24 cm.

Introduction -- Health and Safety Considerations -- Sample Collection and Storage -- Low-Cost DNA Extraction -- PCR Amplification for Low-Cost Mutation Discovery -- Enzymatic Mismatch Cleavage and Agarose Gel Evaluation of Samples -- Alternative Enzymology for Mismatch Cleavage for TILLING and Ecotilling: Extraction of Enzymes form Common Weedy Plants -- Example Data -- Conclusions.

This book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single nucleotide polymorphism; and small insertion/deletion discovery using standard agarose gel electrophoresis. The methods described can be applied in any laboratory equipped for basic molecular biology and do away with the need for expensive freezers and toxic organic compounds. With the appropriate validation of sample quality and longevity, they can provide sufficient DNA for a variety of molecular applications, such as marker studies and TILLING, at approximately one tenth of the cost per sample when compared to commercial kits.

ISBN: 9783319162591 (electronic bk.)

Standard No.: 10.1007/978-3-319-16259-1doiSubjects--Topical Terms:

592577
Plant molecular genetics.

LC Class. No.: QK981.4

Dewey Class. No.: 572.82

Low-cost methods for molecular characterization of mutant plants = tissue desiccation, DNA extraction and mutation discovery: protocols /
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505 0 $a Introduction -- Health and Safety Considerations -- Sample Collection and Storage -- Low-Cost DNA Extraction -- PCR Amplification for Low-Cost Mutation Discovery -- Enzymatic Mismatch Cleavage and Agarose Gel Evaluation of Samples -- Alternative Enzymology for Mismatch Cleavage for TILLING and Ecotilling: Extraction of Enzymes form Common Weedy Plants -- Example Data -- Conclusions.
520 $a This book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single nucleotide polymorphism; and small insertion/deletion discovery using standard agarose gel electrophoresis. The methods described can be applied in any laboratory equipped for basic molecular biology and do away with the need for expensive freezers and toxic organic compounds. With the appropriate validation of sample quality and longevity, they can provide sufficient DNA for a variety of molecular applications, such as marker studies and TILLING, at approximately one tenth of the cost per sample when compared to commercial kits.
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