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Expression of the Artemisia annua be...

Reinsvold, Robert Eric.

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Expression of the Artemisia annua beta-caryophyllene synthase in Synechocystis sp. strain PCC6803 results in cyanobacterial sesquiterpene accumulation.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Expression of the Artemisia annua beta-caryophyllene synthase in Synechocystis sp. strain PCC6803 results in cyanobacterial sesquiterpene accumulation./
Author:	Reinsvold, Robert Eric.
Description:	111 p.
Notes:	Source: Masters Abstracts International, Volume: 49-04, page: 2351.
Contained By:	Masters Abstracts International49-04.
Subject:	Biology, Molecular. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1489166
ISBN:	9781124489148

Expression of the Artemisia annua beta-caryophyllene synthase in Synechocystis sp. strain PCC6803 results in cyanobacterial sesquiterpene accumulation.
Reinsvold, Robert Eric.

Expression of the Artemisia annua beta-caryophyllene synthase in Synechocystis sp. strain PCC6803 results in cyanobacterial sesquiterpene accumulation. - 111 p.

Source: Masters Abstracts International, Volume: 49-04, page: 2351.

Thesis (M.S.)--University of Northern Colorado, 2010.

The sesquiterpene beta-caryophyllene is a ubiquitous component of many plant resins and has been used in the cosmetic industry to provide a woody, spicy aroma. Recent clinical studies have shown its potential effectiveness as an antibiotic, anesthetic, and anti-inflammatory agent. Additionally, there is significant interest in sesquiterpene accumulation in phototrophic microorganisms for the production of biofuels. Currently, the isolation of beta-caryophyllene relies on purification methods from oleoresins extracted from large amounts of plant material. The design of a cyanobacterium platform that produces beta-caryophyllene may provide a more sustainable and controllable means of production, allowing for an increase in yield without the potential deleterious effects to natural sources. To this end, the beta-caryophyllene synthase gene (QHS1 ) from Artemisia annua (Chinese wormwood) was stably inserted via double homologous recombination into the genome of the cyanobacterium Synechocystis sp. strain PCC6803, under the control of the strong endogenous psbAII promoter. Gene insertion into Synechocystis was confirmed through polymerase chain reaction assays and sequencing reactions. Transcription and expression of QHS1 was confirmed using reverse transcription PCR assays and production of beta-caryophyllene was confirmed in the transgenic strain using gas chromatography with flame ionization detection and mass-spectrometry detection.

ISBN: 9781124489148Subjects--Topical Terms:

1017719
Biology, Molecular.

Expression of the Artemisia annua beta-caryophyllene synthase in Synechocystis sp. strain PCC6803 results in cyanobacterial sesquiterpene accumulation.
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020 $a 9781124489148
035 $a (MiAaPQ)AAI1489166
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100 1 $a Reinsvold, Robert Eric. $3 2102743
245 1 0 $a Expression of the Artemisia annua beta-caryophyllene synthase in Synechocystis sp. strain PCC6803 results in cyanobacterial sesquiterpene accumulation.
300 $a 111 p.
500 $a Source: Masters Abstracts International, Volume: 49-04, page: 2351.
500 $a Adviser: Chhandak Basu.
502 $a Thesis (M.S.)--University of Northern Colorado, 2010.
520 $a The sesquiterpene beta-caryophyllene is a ubiquitous component of many plant resins and has been used in the cosmetic industry to provide a woody, spicy aroma. Recent clinical studies have shown its potential effectiveness as an antibiotic, anesthetic, and anti-inflammatory agent. Additionally, there is significant interest in sesquiterpene accumulation in phototrophic microorganisms for the production of biofuels. Currently, the isolation of beta-caryophyllene relies on purification methods from oleoresins extracted from large amounts of plant material. The design of a cyanobacterium platform that produces beta-caryophyllene may provide a more sustainable and controllable means of production, allowing for an increase in yield without the potential deleterious effects to natural sources. To this end, the beta-caryophyllene synthase gene (QHS1 ) from Artemisia annua (Chinese wormwood) was stably inserted via double homologous recombination into the genome of the cyanobacterium Synechocystis sp. strain PCC6803, under the control of the strong endogenous psbAII promoter. Gene insertion into Synechocystis was confirmed through polymerase chain reaction assays and sequencing reactions. Transcription and expression of QHS1 was confirmed using reverse transcription PCR assays and production of beta-caryophyllene was confirmed in the transgenic strain using gas chromatography with flame ionization detection and mass-spectrometry detection.
590 $a School code: 0161.
650 4 $a Biology, Molecular. $3 1017719
650 4 $a Biology, Microbiology. $3 1017734
690 $a 0307
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710 2 $a University of Northern Colorado. $b Biological Science. $3 2102744
773 0 $t Masters Abstracts International $g 49-04.
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792 $a 2010
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1489166