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The effect of freezing rate on strip...

Frankel, Tyler Edward.

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The effect of freezing rate on striped bass (morone saxatilis) spermatozoa.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	The effect of freezing rate on striped bass (morone saxatilis) spermatozoa./
Author:	Frankel, Tyler Edward.
Description:	83 p.
Notes:	Source: Masters Abstracts International, Volume: 52-03.
Contained By:	Masters Abstracts International52-03(E).
Subject:	Agriculture, Animal Culture and Nutrition. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1547722
ISBN:	9781303498855

The effect of freezing rate on striped bass (morone saxatilis) spermatozoa.
Frankel, Tyler Edward.

The effect of freezing rate on striped bass (morone saxatilis) spermatozoa. - 83 p.

Source: Masters Abstracts International, Volume: 52-03.

Thesis (M.S.)--University of Maryland, College Park, 2013.

Several studies have attempted to determine the optimal freezing rate for the cryopreservation of striped bass spermatozoa. In this study, the effects of freezing rate (-10, -15, -20 and -40oC/min) on sperm quality was examined utilizing Sybr- 14/Propidium Iodide staining to confirm membrane integrity, a luciferin-luciferase assay to estimate ATP concentration and a Hamilton-Thorne CEROS(TM) system to characterize sperm motion. Males (n=12) were sampled once a week for five weeks. Samples were extended, cryo-protected using a 7.5% (v/v) dimethyl sulfoxide solution, and frozen using a controlled-rate freezer. Samples were stored in liquid nitrogen for 49 days and re-evaluated post-thaw. Sperm cryopreserved at -40oC per minute resulted in: total motility (10.06%), progressive motility (7.14%), ATP concentration (0.86 pmol/million cells), and sperm viability (56.5%); which were greater (P < 0.05) than the slower rates. Results demonstrate that -40oC/minute was the optimal freezing rate among those tested for the cryopreservation of striped bass spermatozoa.

ISBN: 9781303498855Subjects--Topical Terms:

1017857
Agriculture, Animal Culture and Nutrition.

The effect of freezing rate on striped bass (morone saxatilis) spermatozoa.
LDR:01946nam a2200289 4500 001 1965677
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008 150210s2013 ||||||||||||||||| ||eng d
020 $a 9781303498855
035 $a (MiAaPQ)AAI1547722
035 $a AAI1547722
040 $a MiAaPQ $c MiAaPQ
100 1 $a Frankel, Tyler Edward. $3 2102371
245 1 4 $a The effect of freezing rate on striped bass (morone saxatilis) spermatozoa.
300 $a 83 p.
500 $a Source: Masters Abstracts International, Volume: 52-03.
500 $a Adviser: Lewis C. Woods.
502 $a Thesis (M.S.)--University of Maryland, College Park, 2013.
520 $a Several studies have attempted to determine the optimal freezing rate for the cryopreservation of striped bass spermatozoa. In this study, the effects of freezing rate (-10, -15, -20 and -40oC/min) on sperm quality was examined utilizing Sybr- 14/Propidium Iodide staining to confirm membrane integrity, a luciferin-luciferase assay to estimate ATP concentration and a Hamilton-Thorne CEROS(TM) system to characterize sperm motion. Males (n=12) were sampled once a week for five weeks. Samples were extended, cryo-protected using a 7.5% (v/v) dimethyl sulfoxide solution, and frozen using a controlled-rate freezer. Samples were stored in liquid nitrogen for 49 days and re-evaluated post-thaw. Sperm cryopreserved at -40oC per minute resulted in: total motility (10.06%), progressive motility (7.14%), ATP concentration (0.86 pmol/million cells), and sperm viability (56.5%); which were greater (P < 0.05) than the slower rates. Results demonstrate that -40oC/minute was the optimal freezing rate among those tested for the cryopreservation of striped bass spermatozoa.
590 $a School code: 0117.
650 4 $a Agriculture, Animal Culture and Nutrition. $3 1017857
650 4 $a Agriculture, Fisheries and Aquaculture. $3 1020913
650 4 $a Biology, Physiology. $3 1017816
690 $a 0475
690 $a 0792
690 $a 0719
710 2 $a University of Maryland, College Park. $b Animal Sciences. $3 1023474
773 0 $t Masters Abstracts International $g 52-03(E).
790 $a 0117
791 $a M.S.
792 $a 2013
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1547722