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Side population sorting separates su...

von Furstenberg, Richard Joseph.

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Side population sorting separates subfractions of intestinal stem cells.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Side population sorting separates subfractions of intestinal stem cells./
Author:	von Furstenberg, Richard Joseph.
Description:	45 p.
Notes:	Source: Masters Abstracts International, Volume: 51-06.
Contained By:	Masters Abstracts International51-06(E).
Subject:	Biology, Physiology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1538154
ISBN:	9781303109065

Side population sorting separates subfractions of intestinal stem cells.
von Furstenberg, Richard Joseph.

Side population sorting separates subfractions of intestinal stem cells. - 45 p.

Source: Masters Abstracts International, Volume: 51-06.

Thesis (M.S.)--The University of North Carolina at Chapel Hill, 2013.

The existence of intestinal stem cells (ISCs) which generate the epithelium has been recognized for over three decades. Recent work suggests there are two distinct ISC populations: active ISCs which are important for epithelial homeostasis, and quiescent ISCs that drive proliferation after injury. Although quiescent ISCs may be of greater therapeutic use, to date, isolation of this population has been achieved only with fluorescent reporter mice. My goal was to identify an isolation method for both ISC populations that can be used in wild-type mice and eventually humans. In the following experiments I use side population (SP) sorting to demonstrate that the USP: contains reporter cells of the active ISC, is highly proliferative by S phase analysis, and expresses high levels of the known active ISC transcripts. In contrast the LSP is: non-proliferative, de-enriched for active ISC transcripts, and enriched for those of quiescent ISCs.

ISBN: 9781303109065Subjects--Topical Terms:

1017816
Biology, Physiology.

Side population sorting separates subfractions of intestinal stem cells.
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020 $a 9781303109065
035 $a (MiAaPQ)AAI1538154
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040 $a MiAaPQ $c MiAaPQ
100 1 $a von Furstenberg, Richard Joseph. $3 2102329
245 1 0 $a Side population sorting separates subfractions of intestinal stem cells.
300 $a 45 p.
500 $a Source: Masters Abstracts International, Volume: 51-06.
500 $a Advisers: Susan J. Henning; Michael F. Goy.
502 $a Thesis (M.S.)--The University of North Carolina at Chapel Hill, 2013.
520 $a The existence of intestinal stem cells (ISCs) which generate the epithelium has been recognized for over three decades. Recent work suggests there are two distinct ISC populations: active ISCs which are important for epithelial homeostasis, and quiescent ISCs that drive proliferation after injury. Although quiescent ISCs may be of greater therapeutic use, to date, isolation of this population has been achieved only with fluorescent reporter mice. My goal was to identify an isolation method for both ISC populations that can be used in wild-type mice and eventually humans. In the following experiments I use side population (SP) sorting to demonstrate that the USP: contains reporter cells of the active ISC, is highly proliferative by S phase analysis, and expresses high levels of the known active ISC transcripts. In contrast the LSP is: non-proliferative, de-enriched for active ISC transcripts, and enriched for those of quiescent ISCs.
590 $a School code: 0153.
650 4 $a Biology, Physiology. $3 1017816
650 4 $a Biology, Cell. $3 1017686
650 4 $a Biology, Molecular. $3 1017719
690 $a 0719
690 $a 0379
690 $a 0307
710 2 $a The University of North Carolina at Chapel Hill. $b Cell and Molecular Physiology. $3 2102330
773 0 $t Masters Abstracts International $g 51-06(E).
790 $a 0153
791 $a M.S.
792 $a 2013
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1538154