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Physics and Application of Nanofluid...

Karpusenka, Alena V.

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Physics and Application of Nanofluidic Systems.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Physics and Application of Nanofluidic Systems./
Author:	Karpusenka, Alena V.
Description:	144 p.
Notes:	Source: Dissertation Abstracts International, Volume: 75-03(E), Section: B.
Contained By:	Dissertation Abstracts International75-03B(E).
Subject:	Physics, Molecular. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3575762
ISBN:	9781303547430

Physics and Application of Nanofluidic Systems.
Karpusenka, Alena V.

Physics and Application of Nanofluidic Systems. - 144 p.

Source: Dissertation Abstracts International, Volume: 75-03(E), Section: B.

Thesis (Ph.D.)--North Carolina State University, 2013.

We report results of the three main groups of experiments: DNA bending, fluctuations and single cell mapping.

ISBN: 9781303547430Subjects--Topical Terms:

1018648
Physics, Molecular.

Physics and Application of Nanofluidic Systems.
LDR:03165nam a2200349 4500 001 1965569
005 20141030134121.5
008 150210s2013 ||||||||||||||||| ||eng d
020 $a 9781303547430
035 $a (MiAaPQ)AAI3575762
035 $a AAI3575762
040 $a MiAaPQ $c MiAaPQ
100 1 $a Karpusenka, Alena V. $3 2102248
245 1 0 $a Physics and Application of Nanofluidic Systems.
300 $a 144 p.
500 $a Source: Dissertation Abstracts International, Volume: 75-03(E), Section: B.
500 $a Adviser: Robert Riehn.
502 $a Thesis (Ph.D.)--North Carolina State University, 2013.
520 $a We report results of the three main groups of experiments: DNA bending, fluctuations and single cell mapping.
520 $a In our work on the estimation of the herniation onset, we have observed DNA molecules of various lengths confined to the different nanochannels. We have discovered a certain diviation from the commonly used theories and presented a newly qualitative theory based on the observed results. We have also performed numerical analysis of the energy profile at the junction of the nanochannels in a crisscross lattice. Results qualitatively agree with experimental observations.
520 $a We also performed experimental observation and analysis of the magnitude of length and density fluctuations in DNA that has been stretched to a new equilibrium state in the nanofluidic channels. We found that experimental data agrees with the Rouse model and can be described using a one-dimensional overdamped oscillator chain with nonzero equilibrium spring length. A discussion of how the measurement process would influence the apparent measured dynamic properties was done.
520 $a In the last section, we first report the profiling of the 5-methyl cytosine distribution within single genomic-sized barcode molecules. To achieve gene-relevant resolution, we linearized the molecule by stretching it in a nanochannel and detected the location of the methyl-CpG binding domain proteins (MBD) conjugated with methylated parts of the barcode.
520 $a The same technique was used in the chromatin mapping experiments. We report our work on the detection of the trimethylated H3K4 and acetylated H3K9 histone markers on the three different reconstituted chromatin (calf thymus, HeLa, chicken erythrocyte). We demonstrated successful results in quantification of the relative histone modifications at a single molecule scale.
520 $a Lastly, we report the results of development of the single cell fluidic system, which is able to operate with genetic material after cell lysis is performed on the chip. We also show that cleaning procedure and buffer exchange can be effectively performed on the same chip without extra manipulations to the DNA material, which could result in higher yield and precision of the experimental technique on a single cell level.
590 $a School code: 0155.
650 4 $a Physics, Molecular. $3 1018648
650 4 $a Chemistry, Polymer. $3 1018428
650 4 $a Nanoscience. $3 587832
690 $a 0609
690 $a 0495
690 $a 0565
710 2 $a North Carolina State University. $3 1018772
773 0 $t Dissertation Abstracts International $g 75-03B(E).
790 $a 0155
791 $a Ph.D.
792 $a 2013
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3575762