東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back

Switch To: Labeled | MARC Mode | ISBD

Evaluation of quantitative polymeras...

Wood-Jones, Alicia Kay.

Linked to FindBook

Google Book

Amazon

博客來

Evaluation of quantitative polymerase reaction and microbial volatile organic compounds to determine resistance to Aspergillus flavus in maize.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Evaluation of quantitative polymerase reaction and microbial volatile organic compounds to determine resistance to Aspergillus flavus in maize./
Author:	Wood-Jones, Alicia Kay.
Description:	117 p.
Notes:	Source: Dissertation Abstracts International, Volume: 75-03(E), Section: B.
Contained By:	Dissertation Abstracts International75-03B(E).
Subject:	Agriculture, Plant Pathology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3603491
ISBN:	9781303581601

Evaluation of quantitative polymerase reaction and microbial volatile organic compounds to determine resistance to Aspergillus flavus in maize.
Wood-Jones, Alicia Kay.

Evaluation of quantitative polymerase reaction and microbial volatile organic compounds to determine resistance to Aspergillus flavus in maize. - 117 p.

Source: Dissertation Abstracts International, Volume: 75-03(E), Section: B.

Thesis (Ph.D.)--Mississippi State University, 2013.

Screening for resistance to Aspergillus flavus and aflatoxin contamination in maize is an ongoing effort by universities, state and federal agencies. We evaluated two techniques to screen for resistance; quantitative polymerase reaction (QPCR) and solid-phase microextraction (SPME). Methods were adapted to accurately detect and quantify the fungus in culture and in the vegetative stage of plant tissues. These assays can eliminate microbiological techniques. The primary objectives of the study were to utilize 1) QPCR to detect and quantify fungal biomass in maize stem tissues to evaluate resistance in maize genotypes to A. flavus colonization in situ and in vivo and 2) SPME to identify key MVOC's to differentiate aflatoxigenic and non-aflatoxigenic strains of A. flavus in situ. A novel QPCR TaqMan probe (OMG3) was designed to detect a region in the aflP gene. The OMG3 probe detected 98.3% of the aflatoxigenic strains. The predominant MVOC's extracted from both aflatoxigenic and non-aflatoxigenic strains were alcohols, ketones and hydrocarbons. The aflatoxigenic strain produced 39 compounds and the non-aflatoxigenic strain produced 41 compounds. Dimethylsulfide and 2-heptanol were key MVOC biomarkers produced only by the aflatoxigenic strain of A. flavus. Accuracy of the QPCR OMG3 probe, in vivo and in situ procedures were developed. A toothpick inoculation method was used to artificially inoculate maize stems in the vegetative stage five (V5). Plants were harvested at V7 and sampled at predetermined sites. This method was 91% consistent for infecting maize plants. The OMG3 probe was evaluated in in vivo and in situ studies conducted in the greenhouse, growth chamber, and field. Lesion length was greater in susceptible lines in 4 of 7 greenhouse trials. Based on inoculation data, subsequent research should focus on refining tissue-sampling methods and increasing length of plant growth time for tissue sampling post-inoculation.

ISBN: 9781303581601Subjects--Topical Terms:

1028950
Agriculture, Plant Pathology.

Evaluation of quantitative polymerase reaction and microbial volatile organic compounds to determine resistance to Aspergillus flavus in maize.
LDR:02926nam a2200289 4500 001 1961858
005 20140722092953.5
008 150210s2013 ||||||||||||||||| ||eng d
020 $a 9781303581601
035 $a (MiAaPQ)AAI3603491
035 $a AAI3603491
040 $a MiAaPQ $c MiAaPQ
100 1 $a Wood-Jones, Alicia Kay. $3 2097830
245 1 0 $a Evaluation of quantitative polymerase reaction and microbial volatile organic compounds to determine resistance to Aspergillus flavus in maize.
300 $a 117 p.
500 $a Source: Dissertation Abstracts International, Volume: 75-03(E), Section: B.
500 $a Adviser: Richard R. Baird.
502 $a Thesis (Ph.D.)--Mississippi State University, 2013.
520 $a Screening for resistance to Aspergillus flavus and aflatoxin contamination in maize is an ongoing effort by universities, state and federal agencies. We evaluated two techniques to screen for resistance; quantitative polymerase reaction (QPCR) and solid-phase microextraction (SPME). Methods were adapted to accurately detect and quantify the fungus in culture and in the vegetative stage of plant tissues. These assays can eliminate microbiological techniques. The primary objectives of the study were to utilize 1) QPCR to detect and quantify fungal biomass in maize stem tissues to evaluate resistance in maize genotypes to A. flavus colonization in situ and in vivo and 2) SPME to identify key MVOC's to differentiate aflatoxigenic and non-aflatoxigenic strains of A. flavus in situ. A novel QPCR TaqMan probe (OMG3) was designed to detect a region in the aflP gene. The OMG3 probe detected 98.3% of the aflatoxigenic strains. The predominant MVOC's extracted from both aflatoxigenic and non-aflatoxigenic strains were alcohols, ketones and hydrocarbons. The aflatoxigenic strain produced 39 compounds and the non-aflatoxigenic strain produced 41 compounds. Dimethylsulfide and 2-heptanol were key MVOC biomarkers produced only by the aflatoxigenic strain of A. flavus. Accuracy of the QPCR OMG3 probe, in vivo and in situ procedures were developed. A toothpick inoculation method was used to artificially inoculate maize stems in the vegetative stage five (V5). Plants were harvested at V7 and sampled at predetermined sites. This method was 91% consistent for infecting maize plants. The OMG3 probe was evaluated in in vivo and in situ studies conducted in the greenhouse, growth chamber, and field. Lesion length was greater in susceptible lines in 4 of 7 greenhouse trials. Based on inoculation data, subsequent research should focus on refining tissue-sampling methods and increasing length of plant growth time for tissue sampling post-inoculation.
590 $a School code: 0132.
650 4 $a Agriculture, Plant Pathology. $3 1028950
650 4 $a Chemistry, General. $3 1021807
650 4 $a Biology, Molecular. $3 1017719
690 $a 0480
690 $a 0485
690 $a 0307
710 2 $a Mississippi State University. $b Entomology and Plant Pathology. $3 1671729
773 0 $t Dissertation Abstracts International $g 75-03B(E).
790 $a 0132
791 $a Ph.D.
792 $a 2013
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3603491