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Tracking stem cell differentiation w...

Lu, Jente.

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Tracking stem cell differentiation with novel electrophysiological markers using dielectrophoresis.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Tracking stem cell differentiation with novel electrophysiological markers using dielectrophoresis./
Author:	Lu, Jente.
Description:	229 p.
Notes:	Source: Dissertation Abstracts International, Volume: 73-03, Section: B, page: 1683.
Contained By:	Dissertation Abstracts International73-03B.
Subject:	Engineering, Biomedical. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3487056
ISBN:	9781267058072

Tracking stem cell differentiation with novel electrophysiological markers using dielectrophoresis.
Lu, Jente.

Tracking stem cell differentiation with novel electrophysiological markers using dielectrophoresis. - 229 p.

Source: Dissertation Abstracts International, Volume: 73-03, Section: B, page: 1683.

Thesis (Ph.D.)--University of California, Irvine, 2011.

The relatively new field of neural stem cell biology is hampered by a lack of methods to accurately determine the phenotype of cells. Cell type-specific markers used for flow cytometry or fluorescence-activated cell sorting are limited and often recognize multiple members of the neural stem cell lineage. The goal of my thesis work is to develop a complementary approach that would be less dependent on the identification of particular markers for a subpopulation of cells and more focused on measuring their overall character. We applied the label-free technique dielectrophoresis (DEP), which has existed for over 30 years but was not commonly used in the stem cell field, to the study of neural stem/progenitor cells (NSPCs). Using DEP trapping curves, we found unique dielectric signatures distinguish NSPCs and their differentiated progeny (neurons and astrocytes) as well as NSPCs with distinct fate potential. Furthermore, we identified a population level response to DEP reflecting heterogeneity of cell cultures. We determined that NSPC biophysical properties revealed by DEP reflect the fate potential of both human and mouse NSPCs. In particular, there is an inverse correlation between neuronal fate potential and membrane capacitance. Moreover, cells' membrane capacitance values shift as they differentiate from embryonic stem cells to fully differentiated neural cells. In addition, we verified that DEP exposure is not toxic for NSPCs in the short time scales needed for cell analysis or sorting. However, the toxicity studies led us to devise a DEP process to selectively ablate astrocytic progenitors based on their unique dielectric properties. I have collaborated on studies using intrinsic cell dielectric properties to separate mixtures of NSPCs and neurons and prospectively sort neuronal and astrocytic progenitors from NSPCs. The study has not only uncovered novel, label-free, cell type-specific markers of neural stem cell lineages but has also opened new avenues of biological discovery for stem and progenitor cells.

ISBN: 9781267058072Subjects--Topical Terms:

1017684
Engineering, Biomedical.

Tracking stem cell differentiation with novel electrophysiological markers using dielectrophoresis.
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245 1 0 $a Tracking stem cell differentiation with novel electrophysiological markers using dielectrophoresis.
300 $a 229 p.
500 $a Source: Dissertation Abstracts International, Volume: 73-03, Section: B, page: 1683.
500 $a Advisers: Abraham P. Lee; Lisa A. Flanagan.
502 $a Thesis (Ph.D.)--University of California, Irvine, 2011.
520 $a The relatively new field of neural stem cell biology is hampered by a lack of methods to accurately determine the phenotype of cells. Cell type-specific markers used for flow cytometry or fluorescence-activated cell sorting are limited and often recognize multiple members of the neural stem cell lineage. The goal of my thesis work is to develop a complementary approach that would be less dependent on the identification of particular markers for a subpopulation of cells and more focused on measuring their overall character. We applied the label-free technique dielectrophoresis (DEP), which has existed for over 30 years but was not commonly used in the stem cell field, to the study of neural stem/progenitor cells (NSPCs). Using DEP trapping curves, we found unique dielectric signatures distinguish NSPCs and their differentiated progeny (neurons and astrocytes) as well as NSPCs with distinct fate potential. Furthermore, we identified a population level response to DEP reflecting heterogeneity of cell cultures. We determined that NSPC biophysical properties revealed by DEP reflect the fate potential of both human and mouse NSPCs. In particular, there is an inverse correlation between neuronal fate potential and membrane capacitance. Moreover, cells' membrane capacitance values shift as they differentiate from embryonic stem cells to fully differentiated neural cells. In addition, we verified that DEP exposure is not toxic for NSPCs in the short time scales needed for cell analysis or sorting. However, the toxicity studies led us to devise a DEP process to selectively ablate astrocytic progenitors based on their unique dielectric properties. I have collaborated on studies using intrinsic cell dielectric properties to separate mixtures of NSPCs and neurons and prospectively sort neuronal and astrocytic progenitors from NSPCs. The study has not only uncovered novel, label-free, cell type-specific markers of neural stem cell lineages but has also opened new avenues of biological discovery for stem and progenitor cells.
590 $a School code: 0030.
650 4 $a Engineering, Biomedical. $3 1017684
650 4 $a Biophysics, General. $3 1019105
650 4 $a Engineering, General. $3 1020744
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710 2 $a University of California, Irvine. $b Biomedical Engineering - Ph.D.. $3 2097728
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792 $a 2011
793 $a English
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