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Integrated microfluidic platforms fo...

Kim, Hyung Joon.

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Integrated microfluidic platforms for investigating neuronal networks.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Integrated microfluidic platforms for investigating neuronal networks./
Author:	Kim, Hyung Joon.
Description:	174 p.
Notes:	Source: Dissertation Abstracts International, Volume: 71-04, Section: B, page: 2624.
Contained By:	Dissertation Abstracts International71-04B.
Subject:	Engineering, Materials Science. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3397049
ISBN:	9781109672084

Integrated microfluidic platforms for investigating neuronal networks.
Kim, Hyung Joon.

Integrated microfluidic platforms for investigating neuronal networks. - 174 p.

Source: Dissertation Abstracts International, Volume: 71-04, Section: B, page: 2624.

Thesis (Ph.D.)--University of California, Irvine, 2010.

This dissertation describes the development and application of integrated microfluidics-based assay platforms to study neuronal activities in the nervous system in-vitro. The assay platforms were fabricated using soft lithography and micro/nano fabrication including microfluidics, surface patterning, and nanomaterial synthesis. The use of integrated microfluidics-based assay platform allows culturing and manipulating many types of neuronal tissues in precisely controlled microenvironment. Furthermore, they provide organized multi-cellular in-vitro model, long-term monitoring with live cell imaging, and compatibility with molecular biology techniques and electrophysiology experiment. In this dissertation, the integrated microfluidics-based assay platforms are developed for investigation of neuronal activities such as local protein synthesis, impairment of axonal transport by chemical/physical variants, growth cone path finding under chemical/physical cues, and synaptic transmission in neuronal circuit.

ISBN: 9781109672084Subjects--Topical Terms:

1017759
Engineering, Materials Science.

Integrated microfluidic platforms for investigating neuronal networks.
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020 $a 9781109672084
035 $a (MiAaPQ)AAI3397049
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100 1 $a Kim, Hyung Joon. $3 2097710
245 1 0 $a Integrated microfluidic platforms for investigating neuronal networks.
300 $a 174 p.
500 $a Source: Dissertation Abstracts International, Volume: 71-04, Section: B, page: 2624.
500 $a Advisers: Noo Li Jeon; Abraham P. Lee.
502 $a Thesis (Ph.D.)--University of California, Irvine, 2010.
520 $a This dissertation describes the development and application of integrated microfluidics-based assay platforms to study neuronal activities in the nervous system in-vitro. The assay platforms were fabricated using soft lithography and micro/nano fabrication including microfluidics, surface patterning, and nanomaterial synthesis. The use of integrated microfluidics-based assay platform allows culturing and manipulating many types of neuronal tissues in precisely controlled microenvironment. Furthermore, they provide organized multi-cellular in-vitro model, long-term monitoring with live cell imaging, and compatibility with molecular biology techniques and electrophysiology experiment. In this dissertation, the integrated microfluidics-based assay platforms are developed for investigation of neuronal activities such as local protein synthesis, impairment of axonal transport by chemical/physical variants, growth cone path finding under chemical/physical cues, and synaptic transmission in neuronal circuit.
520 $a Chapter 1 describes the motivation, objectives, and scope for developing in-vitro platform to study various neuronal activities. Chapter 2 introduces microfluidic culture platform for biochemical assay with large-scale neuronal tissues that are utilized as model system in neuroscience research. Chapter 3 focuses on the investigation of impaired axonal transport by beta-Amyloid and oxidative stress. The platform allows to control neuronal processes and to quantify mitochondrial movement in various regions of axons away from applied drugs. Chapter 4 demonstrates the development of microfluidics-based growth cone turning assay to elucidate the mechanism underlying axon guidance under soluble factors and shear flow. Using this platform, the behaviors of growth cone of mammalian neurons are verified under the gradient of inhibitory molecules and also shear flow in well-controlled manner. In Chapter 5, I combine in-vitro multicellular model with microfabricated MEA (multielectrode array) or nanowire electrode array to study electrophysiology in neuronal network. Also, "diode-like" microgrooves to control the number of neuronal processes is embedded in this platform. Chapter 6 concludes with a possible future direction of this work. Interfacing micro/nanotechnology with primary neuron culture would open many doors in fundamental neuroscience research and also biomedical innovation.
590 $a School code: 0030.
650 4 $a Engineering, Materials Science. $3 1017759
650 4 $a Engineering, Biomedical. $3 1017684
650 4 $a Biology, Neurobiology. $3 1681328
690 $a 0794
690 $a 0541
690 $a 0421
710 2 $a University of California, Irvine. $b Materials Science and Engineering - Ph.D.. $3 2094019
773 0 $t Dissertation Abstracts International $g 71-04B.
790 $a 0030
791 $a Ph.D.
792 $a 2010
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3397049