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Nucleotide excision repair of ultrav...

Chelico, Linda.

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Nucleotide excision repair of ultraviolet radiation-treated Beauveria bassiana.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Nucleotide excision repair of ultraviolet radiation-treated Beauveria bassiana./
Author:	Chelico, Linda.
Description:	277 p.
Notes:	Source: Dissertation Abstracts International, Volume: 66-09, Section: B, page: 4618.
Contained By:	Dissertation Abstracts International66-09B.
Subject:	Biology, Microbiology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=NR06225
ISBN:	9780494062258

Nucleotide excision repair of ultraviolet radiation-treated Beauveria bassiana.
Chelico, Linda.

Nucleotide excision repair of ultraviolet radiation-treated Beauveria bassiana. - 277 p.

Source: Dissertation Abstracts International, Volume: 66-09, Section: B, page: 4618.

Thesis (Ph.D.)--The University of Saskatchewan (Canada), 2004.

The entomopathogenic fungus (EPF) Beauveria bassiana has potential to be used as an alternative to chemical insecticides in ecosystems. However, the success of EPF is dependent on environmental factors, such as ultraviolet (UV) radiation. The focus for improving survival of EPF in the field has been the use of sunscreens in the final formulation or screening for isolates naturally more resistant to UV radiation. Although studies with B. bassiana have purported that DNA damage and DNA repair were responsible for conidiospore inactivation there has been no direct evidence.

ISBN: 9780494062258Subjects--Topical Terms:

1017734
Biology, Microbiology.

Nucleotide excision repair of ultraviolet radiation-treated Beauveria bassiana.
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020 $a 9780494062258
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100 1 $a Chelico, Linda. $3 2096837
245 1 0 $a Nucleotide excision repair of ultraviolet radiation-treated Beauveria bassiana.
300 $a 277 p.
500 $a Source: Dissertation Abstracts International, Volume: 66-09, Section: B, page: 4618.
502 $a Thesis (Ph.D.)--The University of Saskatchewan (Canada), 2004.
520 $a The entomopathogenic fungus (EPF) Beauveria bassiana has potential to be used as an alternative to chemical insecticides in ecosystems. However, the success of EPF is dependent on environmental factors, such as ultraviolet (UV) radiation. The focus for improving survival of EPF in the field has been the use of sunscreens in the final formulation or screening for isolates naturally more resistant to UV radiation. Although studies with B. bassiana have purported that DNA damage and DNA repair were responsible for conidiospore inactivation there has been no direct evidence.
520 $a The objectives of this research were to investigate UV-induced damage in and the repair of, UV-C-irradiated spores of B. bassiana and five other EPF, to demonstrate a DNA repair pathway in B. bassiana by mutagenesis, selection and partial characterization of UV-sensitive mutants, and to identify the presence of a nucleotide excision repair (NER) gene from an EPF.
520 $a The NER and photoreactivation (Phr) ability, based on percent survival, of B. bassiana, B. brongniartii, B. nivea, Metarhizium anisopliae, Paecilomyces farinosus, and Verticillium lecanii were studied. Beauveria bassiana and M. anisopliae were the most UV-tolerant EPF tested. The effect of two elevated temperatures, 35 and 50 °C, during UV irradiation, on B. bassiana conidiospores was to decrease percent spore survival more than at 25 °C during 36 J/m2 UV irradiation. The NER capability of B. bassiana differed at the life cycle stages of conidiospore, swollen/germinating conidiospore and blastospore. The results supported the hypothesis that B. bassiana had subsets of the NER pathway. The percent survival of B. bassiana in response to the chemical mutagens 4-nitroquinoline 1-oxide (4NQO) and methyl methanesulfonate (MMS) showed that different types of chemical damage to the DNA were repaired differently.
520 $a The number of CPDs formed in B. bassiana DNA UV irradiated at 720 J/m2 in vitro and 120 J/m2 in vivo was 25 and 11 CPDs/10 kb, respectively. The NER in vivo during a 6-h incubation decreased the CPDs to 3 CPDs/10 kb. During incubation post-UV irradiation the pyrimidine-(6-4)-pyrimidone photoproducts ((6-4)PPs) were repaired faster than CPDs.
520 $a To test the biochemical genetics of NER, B. bassiana conidiospores were treated with MMS and 200 putative mutants were isolated. Three UV sensitive mutants, LC75, LC194, and LC85 were identified. The spontaneous reversion frequencies were 2.5 x 10-6, 4.9 x 10 -6, and 2.8 x 10-6 for LC75, LC194, and LC85, respectively. (Abstract shortened by UMI.).
590 $a School code: 0780.
650 4 $a Biology, Microbiology. $3 1017734
650 4 $a Biology, Molecular. $3 1017719
690 $a 0410
690 $a 0307
710 2 $a The University of Saskatchewan (Canada). $3 1025078
773 0 $t Dissertation Abstracts International $g 66-09B.
790 $a 0780
791 $a Ph.D.
792 $a 2004
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=NR06225