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Statistical protein quantification a...

Clough, Timothy.

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Statistical protein quantification and prioritization in label-free shotgun LC-MS/MS proteomics.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Statistical protein quantification and prioritization in label-free shotgun LC-MS/MS proteomics./
Author:	Clough, Timothy.
Description:	140 p.
Notes:	Source: Dissertation Abstracts International, Volume: 74-03(E), Section: B.
Contained By:	Dissertation Abstracts International74-03B(E).
Subject:	Statistics. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3544043
ISBN:	9781267736093

Statistical protein quantification and prioritization in label-free shotgun LC-MS/MS proteomics.
Clough, Timothy.

Statistical protein quantification and prioritization in label-free shotgun LC-MS/MS proteomics. - 140 p.

Source: Dissertation Abstracts International, Volume: 74-03(E), Section: B.

Thesis (Ph.D.)--Purdue University, 2012.

Mass spectrometry-based proteomics is the current method of choice for identifying and quantifying the proteome of an organism. Because no prior knowledge is required, the label-free liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) "shotgun" workflow is widely used for discovery investigations, a typical goal of which is to identify candidate biomarkers of disease.

ISBN: 9781267736093Subjects--Topical Terms:

517247
Statistics.

Statistical protein quantification and prioritization in label-free shotgun LC-MS/MS proteomics.
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020 $a 9781267736093
035 $a (UMI)AAI3544043
035 $a AAI3544043
040 $a UMI $c UMI
100 1 $a Clough, Timothy. $3 2092565
245 1 0 $a Statistical protein quantification and prioritization in label-free shotgun LC-MS/MS proteomics.
300 $a 140 p.
500 $a Source: Dissertation Abstracts International, Volume: 74-03(E), Section: B.
500 $a Adviser: Olga Vitek.
502 $a Thesis (Ph.D.)--Purdue University, 2012.
520 $a Mass spectrometry-based proteomics is the current method of choice for identifying and quantifying the proteome of an organism. Because no prior knowledge is required, the label-free liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) "shotgun" workflow is widely used for discovery investigations, a typical goal of which is to identify candidate biomarkers of disease.
520 $a A challenge of addressing this goal in the LC-MS/MS workflow is that mass spectrometry-based experiments generate measurements not on intact proteins, but on fragments of a protein called peptides. Additionally, the workflow is biased towards the more abundant proteins in a biological sample, leaving potentially interesting lower abundant proteins undetected. The incomplete representation of the proteome can prohibit the discovery of potentially important candidates, severely limiting the usefulness of LC-MS/MS for biomarker discovery.
520 $a This dissertation introduces probabilistic models for addressing these challenges. Through the use of multiple case studies of proteomic investigations, we show that the models are more sensitive and specific for detecting changes in protein abundance than existing methods, and that they can help interpret these changes at a network level, which, with the aid of efficient computational algorithms, helps to prioritize putative disease-associated genes.
590 $a School code: 0183.
650 4 $a Statistics. $3 517247
650 4 $a Biology, Bioinformatics. $3 1018415
690 $a 0463
690 $a 0715
710 2 $a Purdue University. $b Statistics. $3 1279962
773 0 $t Dissertation Abstracts International $g 74-03B(E).
790 1 0 $a Vitek, Olga, $e advisor
790 1 0 $a Brusniak, Mi-Youn $e committee member
790 1 0 $a Craig, Bruce $e committee member
790 1 0 $a Zhu, Michael $e committee member
790 $a 0183
791 $a Ph.D.
792 $a 2012
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3544043