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The significance of metallothionein ...

Dutta, Rana.

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The significance of metallothionein isoform 3 gene expression in human prostate cancer.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	The significance of metallothionein isoform 3 gene expression in human prostate cancer./
作者:	Dutta, Rana.
面頁冊數:	146 p.
附註:	Source: Dissertation Abstracts International, Volume: 63-09, Section: B, page: 4032.
Contained By:	Dissertation Abstracts International63-09B.
標題:	Biology, Genetics. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3064584
ISBN:	0493835687

The significance of metallothionein isoform 3 gene expression in human prostate cancer.
Dutta, Rana.

The significance of metallothionein isoform 3 gene expression in human prostate cancer. - 146 p.

Source: Dissertation Abstracts International, Volume: 63-09, Section: B, page: 4032.

Thesis (Ph.D.)--West Virginia University, 2002.

Since the expression of the brain specific metallothionein isoform, MT-3, was shown to be expressed in many, but not all prostate cancers, this gene was transfected into the commonly utilized prostate tumor cell line, PC-3, to study the consequence of MT-3 expression on growth rate and resistance to chemotherapeutic agents.

ISBN: 0493835687Subjects--Topical Terms:

1017730
Biology, Genetics.

The significance of metallothionein isoform 3 gene expression in human prostate cancer.
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245 1 4 $a The significance of metallothionein isoform 3 gene expression in human prostate cancer.
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500 $a Source: Dissertation Abstracts International, Volume: 63-09, Section: B, page: 4032.
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502 $a Thesis (Ph.D.)--West Virginia University, 2002.
520 $a Since the expression of the brain specific metallothionein isoform, MT-3, was shown to be expressed in many, but not all prostate cancers, this gene was transfected into the commonly utilized prostate tumor cell line, PC-3, to study the consequence of MT-3 expression on growth rate and resistance to chemotherapeutic agents.
520 $a PC-3 cells were stably transfected with MT-3 or MT-1E under control of the constitutive CMV promoter. The levels of MT-3 protein for the 5 overexpressing clones had an average level of 8.3 ng MT-3 per mug protein as assessed by quantitative immunodot blot analysis, and three were picked for further analysis. MT-3 expressing clones had a slower growth rate (63 hr doubling time) compared to vector-only transfected cells (35 hr doubling time). The MT-1E overexpressing clones had total MT-1/2 levels of 5.54 +/- 0.85 ng/mug of protein whereas clones from vector-only transfection had 0.90 +/- 0.05 ng/mug protein. The mean doubling time of the three independent clones of PC-3 cells overexpressing MT-1E was 39.2 hr compared to 36.7 hr for control PC-3 cells, and 39.9 hr for blank vector transfectants.
520 $a The effect of these two isoforms of MT on cellular resistance to commonly utilized chemotherapeutic agents was determined on MT-3 and MT-1E expressing and non-expressing clones of PC-3 cells exposed to cadmium, cisplatin, vinblastine, paclitaxel, etoposide, and mitoxantrone. Both MT-3 and MT-1E expression in PC-3 cells conferred resistance to all five chemotherapeutic agents, with a 10-fold increase in resistance to paclitaxel and mitoxantrone for the MT-1E transfected clones compared MT-3 transfected clones.
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