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Analysis of retroviral production an...

Kwon, Young Jik.

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Analysis of retroviral production and transduction systems.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Analysis of retroviral production and transduction systems./
Author:	Kwon, Young Jik.
Description:	162 p.
Notes:	Source: Dissertation Abstracts International, Volume: 64-09, Section: B, page: 4494.
Contained By:	Dissertation Abstracts International64-09B.
Subject:	Engineering, Chemical. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3103924

Analysis of retroviral production and transduction systems.
Kwon, Young Jik.

Analysis of retroviral production and transduction systems. - 162 p.

Source: Dissertation Abstracts International, Volume: 64-09, Section: B, page: 4494.

Thesis (Ph.D.)--University of Southern California, 2003.

Gene therapy has been focused as one of the most feasible strategies in curing fatal diseases by correcting genetic disorders or introducing new genetic information. Retroviral gene delivery systems consist of two distinct steps: Vector preparation and gene transfer. As an emerging technology, gene therapy processes have not been optimized completely to promise clinical successes, which motivated this study.Subjects--Topical Terms:

1018531
Engineering, Chemical.

Analysis of retroviral production and transduction systems.
LDR:03157nmm 2200301 4500 001 1865305
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008 130614s2003 eng d
035 $a (UnM)AAI3103924
035 $a AAI3103924
040 $a UnM $c UnM
100 1 $a Kwon, Young Jik. $3 1952757
245 1 0 $a Analysis of retroviral production and transduction systems.
300 $a 162 p.
500 $a Source: Dissertation Abstracts International, Volume: 64-09, Section: B, page: 4494.
500 $a Adviser: Ching-An Peng.
502 $a Thesis (Ph.D.)--University of Southern California, 2003.
520 $a Gene therapy has been focused as one of the most feasible strategies in curing fatal diseases by correcting genetic disorders or introducing new genetic information. Retroviral gene delivery systems consist of two distinct steps: Vector preparation and gene transfer. As an emerging technology, gene therapy processes have not been optimized completely to promise clinical successes, which motivated this study.
520 $a A mathematical model was set up to characterize and the compare various retroviral production systems. Results showed higher and more stable production of ecotropic retrovirus from GP+E86/LNCX producer cells than vesicular stomatitis virus glycoprotein (VSV-G) pseudotyped retrovirus from 293GPG/EGFP producer cells. It was also shown that 10% FBS and 37°C is the optimized conditions to get maximized number of infectious ecotropic retrovirus for long-term production. These findings were applied to design a production system of circulating culture medium through cold reservoir.
520 $a Retroviral transduction systems were analyzed and infectious retroviral concentration and transduction rate constant were determined by curve-fitting experimental data to the mathematical model. The result showed two-order of magnitude difference of infectious retroviral concentration from the titer. From the analysis developed in this study, an efficiency parameter representing tranduction rate constant was extracted and showed its usefulness in quantifying various retroviral transduction system under various conditions. VSV-G pseudotyped retrovirus has shown to be more efficient vector than ecotropic and amphotropic retrovirus by comparing transductin rate constants. By analyzing the effect of factors involved in retroviral transduction, it was shown that Polybrene increased the efficiency with higher concentration, while chondroitin sulfate C increases nonspecific binding efficiency at low concentration but decreases at high concentration. Furthermore, the correlation of amphotropic retroviral transduction efficiency with receptor expression was clearly verified under phosphate-depleted condition.
520 $a This study clearly showed the usefulness of engineering approach to retroviral production and transduction systems in maximizing the efficiency.
590 $a School code: 0208.
650 4 $a Engineering, Chemical. $3 1018531
650 4 $a Engineering, Biomedical. $3 1017684
690 $a 0542
690 $a 0541
710 2 0 $a University of Southern California. $3 700129
773 0 $t Dissertation Abstracts International $g 64-09B.
790 1 0 $a Peng, Ching-An, $e advisor
790 $a 0208
791 $a Ph.D.
792 $a 2003
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3103924