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The role of flagella in the attachme...

Smith, Lisa Velsor.

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The role of flagella in the attachment of bacteria to an abiotic surface.

Record Type:	Electronic resources : Monograph/item
Title/Author:	The role of flagella in the attachment of bacteria to an abiotic surface./
Author:	Smith, Lisa Velsor.
Description:	228 p.
Notes:	Source: Dissertation Abstracts International, Volume: 63-12, Section: B, page: 5975.
Contained By:	Dissertation Abstracts International63-12B.
Subject:	Engineering, Chemical. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3073606
ISBN:	0493936181

The role of flagella in the attachment of bacteria to an abiotic surface.
Smith, Lisa Velsor.

The role of flagella in the attachment of bacteria to an abiotic surface. - 228 p.

Source: Dissertation Abstracts International, Volume: 63-12, Section: B, page: 5975.

Thesis (Ph.D.)--University of Virginia, 2003.

Flagella have been implicated in the attachment of motile bacteria to abiotic surfaces due to their physical and chemical properties as well as their ability to propel a cell from the bulk fluid to a surface. In this study, smooth-swimming, nonflagellated, paralyzed, tumbly, and straight flagella mutants of Escherichia coli were examined along with a wild-type strain so that the importance of different aspects of flagellar structure and function to attachment could be inferred from comparisons with each other.

ISBN: 0493936181Subjects--Topical Terms:

1018531
Engineering, Chemical.

The role of flagella in the attachment of bacteria to an abiotic surface.
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100 1 $a Smith, Lisa Velsor. $3 1952707
245 1 4 $a The role of flagella in the attachment of bacteria to an abiotic surface.
300 $a 228 p.
500 $a Source: Dissertation Abstracts International, Volume: 63-12, Section: B, page: 5975.
500 $a Adviser: Roseanne M. Ford.
502 $a Thesis (Ph.D.)--University of Virginia, 2003.
520 $a Flagella have been implicated in the attachment of motile bacteria to abiotic surfaces due to their physical and chemical properties as well as their ability to propel a cell from the bulk fluid to a surface. In this study, smooth-swimming, nonflagellated, paralyzed, tumbly, and straight flagella mutants of Escherichia coli were examined along with a wild-type strain so that the importance of different aspects of flagellar structure and function to attachment could be inferred from comparisons with each other.
520 $a With total internal reflection aqueous fluorescence microscopy (TIRAF) we were able to observe E. coli as they swam near and interacted with a quartz surface while also determining the separation distance between cells and the surface. The observed near-surface cell behaviors were categorized as one of the following based on if and how the cell body was moving: immobile, loosely attached, spinning, swimming, or free. The determined separation distances depended on behavior and strain and varied from 31 to 110 nanometers. Because these distances are greater than that predicted for bacterial attachment with the theory of Derjaguin, Landau, Verwey, and Overbeek, we investigated several potential sources of error for the TIRAF technique.
520 $a Using knowledge about the properties and function of E. coli flagella we were able to explain differences in the proportion of cells observed performing the different behaviors for each strain, and we assert that the rotation of individual filaments and the changing of rotational direction are most important for secure attachment. In general, cells exhibiting behaviors with stronger interactions were closer to the surface and showed less variation in separation distance over time. However, attached motile cells were farther away and showed more variation in separation distance over time than attached nonmotile cells. These results will be important in developing and testing more comprehensive mathematical models of cell/surface interactions.
590 $a School code: 0246.
650 4 $a Engineering, Chemical. $3 1018531
650 4 $a Biology, Microbiology. $3 1017734
690 $a 0542
690 $a 0410
710 2 0 $a University of Virginia. $3 645578
773 0 $t Dissertation Abstracts International $g 63-12B.
790 1 0 $a Ford, Roseanne M., $e advisor
790 $a 0246
791 $a Ph.D.
792 $a 2003
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3073606