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Genome wide transcriptional comparis...

Saldanha, Alok Jerome.

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Genome wide transcriptional comparison of batch and chemostat nutrient limited cultures of Saccharomyces cerevisiae.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Genome wide transcriptional comparison of batch and chemostat nutrient limited cultures of Saccharomyces cerevisiae./
Author:	Saldanha, Alok Jerome.
Description:	176 p.
Notes:	Source: Dissertation Abstracts International, Volume: 64-11, Section: B, page: 5346.
Contained By:	Dissertation Abstracts International64-11B.
Subject:	Biology, Microbiology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3111789

Genome wide transcriptional comparison of batch and chemostat nutrient limited cultures of Saccharomyces cerevisiae.
Saldanha, Alok Jerome.

Genome wide transcriptional comparison of batch and chemostat nutrient limited cultures of Saccharomyces cerevisiae. - 176 p.

Source: Dissertation Abstracts International, Volume: 64-11, Section: B, page: 5346.

Thesis (Ph.D.)--Stanford University, 2004.

The chemostat, a continuous culture system, may provide a better controlled environment for detailed studies using highly sensitive, genome-wide techniques than the widely used batch culture. However, the relation and relevance of the chemostat to batch culture has not been fully characterized. In the first chapter of this thesis I use genome-wide transcriptional profiling to compare batch timepoints and a steady-state chemostat culture under phosphate, sulfate, leucine and uracil limitation. The profiles, together with physiological data, show that phosphate and sulfate limited batch cultures maintain homeostasis as the limiting nutrient is depleted, and are comparable to the chemostat near the point when the limiting nutrient is exhausted. Importantly, there is not a stress response in the chemostat. Leucine and uracil limited chemostat cultures also appear to lack a stress response, but the existence of a comparable point is more difficult to ascertain. Comparison of changes across the timecourses revealed induction of genes involved in the metabolism of the limiting nutrient for most nutrients, but there was no relevant response found for uracil limitation. Several other clusters specific to particular limitations provide insight into the response to those limitations. In the second chapter, I present the design and implementation of Java Treeview, a general, cross platform, open source visualization program for genome-wide microarray data.Subjects--Topical Terms:

1017734
Biology, Microbiology.

Genome wide transcriptional comparison of batch and chemostat nutrient limited cultures of Saccharomyces cerevisiae.
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040 $a UnM $c UnM
100 1 $a Saldanha, Alok Jerome. $3 1952451
245 1 0 $a Genome wide transcriptional comparison of batch and chemostat nutrient limited cultures of Saccharomyces cerevisiae.
300 $a 176 p.
500 $a Source: Dissertation Abstracts International, Volume: 64-11, Section: B, page: 5346.
500 $a Adviser: David Botstein.
502 $a Thesis (Ph.D.)--Stanford University, 2004.
520 $a The chemostat, a continuous culture system, may provide a better controlled environment for detailed studies using highly sensitive, genome-wide techniques than the widely used batch culture. However, the relation and relevance of the chemostat to batch culture has not been fully characterized. In the first chapter of this thesis I use genome-wide transcriptional profiling to compare batch timepoints and a steady-state chemostat culture under phosphate, sulfate, leucine and uracil limitation. The profiles, together with physiological data, show that phosphate and sulfate limited batch cultures maintain homeostasis as the limiting nutrient is depleted, and are comparable to the chemostat near the point when the limiting nutrient is exhausted. Importantly, there is not a stress response in the chemostat. Leucine and uracil limited chemostat cultures also appear to lack a stress response, but the existence of a comparable point is more difficult to ascertain. Comparison of changes across the timecourses revealed induction of genes involved in the metabolism of the limiting nutrient for most nutrients, but there was no relevant response found for uracil limitation. Several other clusters specific to particular limitations provide insight into the response to those limitations. In the second chapter, I present the design and implementation of Java Treeview, a general, cross platform, open source visualization program for genome-wide microarray data.
590 $a School code: 0212.
650 4 $a Biology, Microbiology. $3 1017734
650 4 $a Biology, Molecular. $3 1017719
650 4 $a Biology, Genetics. $3 1017730
690 $a 0410
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710 2 0 $a Stanford University. $3 754827
773 0 $t Dissertation Abstracts International $g 64-11B.
790 1 0 $a Botstein, David, $e advisor
790 $a 0212
791 $a Ph.D.
792 $a 2004
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3111789