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Enhanced Raman scattering of biologi...

Montoya, Joseph R.

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Enhanced Raman scattering of biological molecules.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Enhanced Raman scattering of biological molecules./
Author:	Montoya, Joseph R.
Description:	146 p.
Notes:	Source: Dissertation Abstracts International, Volume: 64-12, Section: B, page: 6145.
Contained By:	Dissertation Abstracts International64-12B.
Subject:	Physics, Optics. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3116351

Enhanced Raman scattering of biological molecules.
Montoya, Joseph R.

Enhanced Raman scattering of biological molecules. - 146 p.

Source: Dissertation Abstracts International, Volume: 64-12, Section: B, page: 6145.

Thesis (Ph.D.)--New Mexico State University, 2003.

The results presented in this thesis, originate from the aspiration to develop an identification algorithm for Salmonella enterica Serovar Enteritidis (S. enterica), Escherichia coli (E. coli), Bacillus globigii ( B. globigii), and Bacillus megaterium ( B. megaterium) using "enhanced" Raman scattering. We realized our goal, with a method utilizing an immunoassay process in a spectroscopic technique, and the direct use of the enhanced spectral response due to bacterial surface elements. The enhanced Raman signal originates from Surface Enhanced Raman Scattering (SERS) and/or Morphological Dependent Resonances (MDR's). We utilized a modified Lee-Meisel colloidal production method to produce a SERS active substrate, which was applied to a SERS application for the amino acid Glycine. The comparison indicates that the SERS/FRACTAL/MDR process can produce an increase of 107 times more signal than the bulk Raman signal from Glycine. In the extension of the Glycine results, we studied the use of SERS related to S. enterica, where we have shown that the aromatic amino acid contribution from Phenylalanine, Tyrosine, and Tryptophan produces a SERS response that can be used to identify the associated SERS vibrational modes of a S. enterica one or two antibody complexes. The "fingerprint" associated with the spectral signature in conjunction with an enhanced Raman signal allows conclusions to be made: (1) about the orientation of the secondary structure on the metal; (2) whether bound/unbound antibody can be neglected; (3) whether we can lower the detection limit. We have lowered the detection limit of S. enterica to 106 bacteria/ml. We also show a profound difference between S. enterica and E. coli SERS spectra even when there exists non-specific binding on E. coli indicating a protein conformation change induced by the addition of the antigen S. enterica. We confirm TEM imagery data, indicating that the source of the aromatic amino acid SERS response is originating from fractal structures on the surface of the bacteria with appropriate associated absorption spectra. In addition, we show that SERS may be used by directly detecting cell surface chemistry, with a report of a SERS response from gram-positive bacteria, B. globigii and B. megaterium combined, with silver fractal aggregates.Subjects--Topical Terms:

1018756
Physics, Optics.

Enhanced Raman scattering of biological molecules.
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035 $a (UnM)AAI3116351
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100 1 $a Montoya, Joseph R. $3 1952001
245 1 0 $a Enhanced Raman scattering of biological molecules.
300 $a 146 p.
500 $a Source: Dissertation Abstracts International, Volume: 64-12, Section: B, page: 6145.
500 $a Chair: Robert Armstrong.
502 $a Thesis (Ph.D.)--New Mexico State University, 2003.
520 $a The results presented in this thesis, originate from the aspiration to develop an identification algorithm for Salmonella enterica Serovar Enteritidis (S. enterica), Escherichia coli (E. coli), Bacillus globigii ( B. globigii), and Bacillus megaterium ( B. megaterium) using "enhanced" Raman scattering. We realized our goal, with a method utilizing an immunoassay process in a spectroscopic technique, and the direct use of the enhanced spectral response due to bacterial surface elements. The enhanced Raman signal originates from Surface Enhanced Raman Scattering (SERS) and/or Morphological Dependent Resonances (MDR's). We utilized a modified Lee-Meisel colloidal production method to produce a SERS active substrate, which was applied to a SERS application for the amino acid Glycine. The comparison indicates that the SERS/FRACTAL/MDR process can produce an increase of 107 times more signal than the bulk Raman signal from Glycine. In the extension of the Glycine results, we studied the use of SERS related to S. enterica, where we have shown that the aromatic amino acid contribution from Phenylalanine, Tyrosine, and Tryptophan produces a SERS response that can be used to identify the associated SERS vibrational modes of a S. enterica one or two antibody complexes. The "fingerprint" associated with the spectral signature in conjunction with an enhanced Raman signal allows conclusions to be made: (1) about the orientation of the secondary structure on the metal; (2) whether bound/unbound antibody can be neglected; (3) whether we can lower the detection limit. We have lowered the detection limit of S. enterica to 106 bacteria/ml. We also show a profound difference between S. enterica and E. coli SERS spectra even when there exists non-specific binding on E. coli indicating a protein conformation change induced by the addition of the antigen S. enterica. We confirm TEM imagery data, indicating that the source of the aromatic amino acid SERS response is originating from fractal structures on the surface of the bacteria with appropriate associated absorption spectra. In addition, we show that SERS may be used by directly detecting cell surface chemistry, with a report of a SERS response from gram-positive bacteria, B. globigii and B. megaterium combined, with silver fractal aggregates.
590 $a School code: 0143.
650 4 $a Physics, Optics. $3 1018756
650 4 $a Biology, Microbiology. $3 1017734
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710 2 0 $a New Mexico State University. $3 783784
773 0 $t Dissertation Abstracts International $g 64-12B.
790 1 0 $a Armstrong, Robert, $e advisor
790 $a 0143
791 $a Ph.D.
792 $a 2003
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3116351