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Cloning and targeted mutagenesis of ...

Ouyang, Ling.

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Cloning and targeted mutagenesis of a recA gene required for the extreme DNA damage-resistance of Deinococcus radiodurans.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Cloning and targeted mutagenesis of a recA gene required for the extreme DNA damage-resistance of Deinococcus radiodurans./
Author:	Ouyang, Ling.
Description:	53 p.
Notes:	Source: Masters Abstracts International, Volume: 33-06, page: 1786.
Contained By:	Masters Abstracts International33-06.
Subject:	Biology, Microbiology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1361765

Cloning and targeted mutagenesis of a recA gene required for the extreme DNA damage-resistance of Deinococcus radiodurans.
Ouyang, Ling.

Cloning and targeted mutagenesis of a recA gene required for the extreme DNA damage-resistance of Deinococcus radiodurans. - 53 p.

Source: Masters Abstracts International, Volume: 33-06, page: 1786.

Thesis (M.S.)--The American University, 1994.

Deinococcus (D.) radiodurans and other members of the same bacterial family are extremely resistant to ionizing radiation, ultraviolet (UV)-radiation and many other DNA-damaging chemical agents. This unusual resistance stems from their extraordinarily efficient DNA repair mechanisms. A mutant strain (strain rec30) generated through chemical mutagenesis has been found to be both DNA-damage sensitive and natural transformation-defficient. Through gene complementation with wild-type (wt) genomic DNA, a gene with extended homology to the Escherichia coli recA was cloned. Transformation of the cloned deinococccal recA gene restored both the DNA-damage resistance and full natural transformation competence of the rec30 mutant. Targeted insertional mutagenesis of the deinococccal recA gene in wt D. radiodurans generated a mutant strain that was phenotypically indistinguishable from strain rec30. These results indicate that the observed phenotypic changes in strain rec30 were caused by a defect in its recA gene, and a RecA-mediated recombination repair pathway plays a critical role in the DNA repair of D. radiodurans.Subjects--Topical Terms:

1017734
Biology, Microbiology.

Cloning and targeted mutagenesis of a recA gene required for the extreme DNA damage-resistance of Deinococcus radiodurans.
LDR:01920nmm 2200241 4500 001 1862045
005 20041215074551.5
008 130614s1994 eng d
035 $a (UnM)AAI1361765
035 $a AAI1361765
040 $a UnM $c UnM
100 1 $a Ouyang, Ling. $3 1949616
245 1 0 $a Cloning and targeted mutagenesis of a recA gene required for the extreme DNA damage-resistance of Deinococcus radiodurans.
300 $a 53 p.
500 $a Source: Masters Abstracts International, Volume: 33-06, page: 1786.
502 $a Thesis (M.S.)--The American University, 1994.
520 $a Deinococcus (D.) radiodurans and other members of the same bacterial family are extremely resistant to ionizing radiation, ultraviolet (UV)-radiation and many other DNA-damaging chemical agents. This unusual resistance stems from their extraordinarily efficient DNA repair mechanisms. A mutant strain (strain rec30) generated through chemical mutagenesis has been found to be both DNA-damage sensitive and natural transformation-defficient. Through gene complementation with wild-type (wt) genomic DNA, a gene with extended homology to the Escherichia coli recA was cloned. Transformation of the cloned deinococccal recA gene restored both the DNA-damage resistance and full natural transformation competence of the rec30 mutant. Targeted insertional mutagenesis of the deinococccal recA gene in wt D. radiodurans generated a mutant strain that was phenotypically indistinguishable from strain rec30. These results indicate that the observed phenotypic changes in strain rec30 were caused by a defect in its recA gene, and a RecA-mediated recombination repair pathway plays a critical role in the DNA repair of D. radiodurans.
590 $a School code: 0008.
650 4 $a Biology, Microbiology. $3 1017734
650 4 $a Biology, Molecular. $3 1017719
690 $a 0410
690 $a 0307
710 2 0 $a The American University. $3 1017567
773 0 $t Masters Abstracts International $g 33-06.
790 $a 0008
791 $a M.S.
792 $a 1994
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1361765