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Linking microbial populations and ge...

Macur, Richard Eugene.

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Linking microbial populations and geochemical processes in soils, mine tailings, and geothermal environments.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Linking microbial populations and geochemical processes in soils, mine tailings, and geothermal environments./
Author:	Macur, Richard Eugene.
Description:	147 p.
Notes:	Source: Dissertation Abstracts International, Volume: 65-03, Section: B, page: 1205.
Contained By:	Dissertation Abstracts International65-03B.
Subject:	Biogeochemistry. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3127710

Linking microbial populations and geochemical processes in soils, mine tailings, and geothermal environments.
Macur, Richard Eugene.

Linking microbial populations and geochemical processes in soils, mine tailings, and geothermal environments. - 147 p.

Source: Dissertation Abstracts International, Volume: 65-03, Section: B, page: 1205.

Thesis (Ph.D.)--Montana State University, 2004.

The primary goal of this work was to identify and characterize the microbial populations responsible for transformations of As and 2,4-D in soils and waters. Chemical, spectroscopic, and microscopic techniques were used to characterize the aqueous and solid phase geochemistry of soils, mine tailings, and a geothermal spring. The role of specific microbial populations in these systems was examined using cultivation-independent molecular methods [total DNA extraction, 16S rDNA amplification, denaturing gradient gel electrophoresis (DGGE), and sequence analysis] coupled with either characterization of microorganisms isolated from the same systems, or inference of physiological characteristics from (i) closely related (16S rDNA sequence) cultured microorganisms and (ii) the geochemical environments in which they were detected.Subjects--Topical Terms:

545717
Biogeochemistry.

Linking microbial populations and geochemical processes in soils, mine tailings, and geothermal environments.
LDR:03516nmm 2200325 4500 001 1862041
005 20041215074551.5
008 130614s2004 eng d
035 $a (UnM)AAI3127710
035 $a AAI3127710
040 $a UnM $c UnM
100 1 $a Macur, Richard Eugene. $3 1949612
245 1 0 $a Linking microbial populations and geochemical processes in soils, mine tailings, and geothermal environments.
300 $a 147 p.
500 $a Source: Dissertation Abstracts International, Volume: 65-03, Section: B, page: 1205.
500 $a Adviser: William P. Inskeep.
502 $a Thesis (Ph.D.)--Montana State University, 2004.
520 $a The primary goal of this work was to identify and characterize the microbial populations responsible for transformations of As and 2,4-D in soils and waters. Chemical, spectroscopic, and microscopic techniques were used to characterize the aqueous and solid phase geochemistry of soils, mine tailings, and a geothermal spring. The role of specific microbial populations in these systems was examined using cultivation-independent molecular methods [total DNA extraction, 16S rDNA amplification, denaturing gradient gel electrophoresis (DGGE), and sequence analysis] coupled with either characterization of microorganisms isolated from the same systems, or inference of physiological characteristics from (i) closely related (16S rDNA sequence) cultured microorganisms and (ii) the geochemical environments in which they were detected.
520 $a The microbial reduction of As(V) to As(III) and the subsequent effects on As mobilization in contaminated mine tailings was examined under transport conditions. Enhanced elution of As from mine tailings apparently resulted from the enrichment of aerobic As(V)-reducing Caulobacter, Sphingomonas , and Rhizobium-like populations after liming.
520 $a Arsenite was rapidly oxidized to As(V) via microbial activity in unsaturated Madison River Valley soil columns. Eight aerobic heterotrophic bacteria with varying As redox phenotypes were isolated from these columns. Three isolates, identified as Agrobacterium tumefaciens-, Pseudomonas fluorescens-, and Variovorax paradoxus-like organisms, were As(III) oxidizers and all were apparently dominant members of the microbial community.
520 $a Successional changes in microbial communities colonizing an As-rich acid-sulfate-chloride geothermal spring stream channel in Norris Geyser Basin of Yellowstone National Park were examined. Enhanced As(III) oxidation correlated in time and space with the appearance of three Hydrogenobaculum-like populations. The formation of an As(V)-rich hydrous-ferric-oxide mat correlated with the detection of Thiomonas, Acidimicrobium, and Metallosphaera ---like populations whose nearest cultivated relatives were Fe-oxidizers.
520 $a Fingerprints of microbial communities (DGGE) established under increasing concentrations of 2,4-D (0--500 mg kg-1) in batch microcosms showed that at least 100 mg kg-1 2,4-D was required to obtain apparent shifts in the community structure. The microbial community selected at high 2,4-D concentrations was predominantly composed of Burkholderia-like populations, which harbored homologs of tfdA genes.
590 $a School code: 0137.
650 4 $a Biogeochemistry. $3 545717
650 4 $a Environmental Sciences. $3 676987
650 4 $a Biology, Ecology. $3 1017726
690 $a 0425
690 $a 0768
690 $a 0329
710 2 0 $a Montana State University. $3 1255135
773 0 $t Dissertation Abstracts International $g 65-03B.
790 1 0 $a Inskeep, William P., $e advisor
790 $a 0137
791 $a Ph.D.
792 $a 2004
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3127710