東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back

Switch To: Labeled | MARC Mode | ISBD

Whole-genome shotgun optical mapping...

Qi, Rong.

Linked to FindBook

Google Book

Amazon

博客來

Whole-genome shotgun optical mapping of the Deinococcus radiodurans and Trypanosoma brucei genomes; and the development of a new surface system for optical mapping.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Whole-genome shotgun optical mapping of the Deinococcus radiodurans and Trypanosoma brucei genomes; and the development of a new surface system for optical mapping./
Author:	Qi, Rong.
Description:	200 p.
Notes:	Source: Dissertation Abstracts International, Volume: 60-09, Section: B, page: 4390.
Contained By:	Dissertation Abstracts International60-09B.
Subject:	Biology, Genetics. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9945317
ISBN:	0599472979

Whole-genome shotgun optical mapping of the Deinococcus radiodurans and Trypanosoma brucei genomes; and the development of a new surface system for optical mapping.
Qi, Rong.

Whole-genome shotgun optical mapping of the Deinococcus radiodurans and Trypanosoma brucei genomes; and the development of a new surface system for optical mapping. - 200 p.

Source: Dissertation Abstracts International, Volume: 60-09, Section: B, page: 4390.

Thesis (Ph.D.)--New York University, 1999.

Optical Mapping is a proven means for the rapid construction of accurate, ordered restriction maps from ensembles of individual surface-mounted DNA molecules. Whole genome shotgun Optical Mapping was used to analyze the genome of Deinococcus radiodurans, Trypanosoma brucei and Homo sapiens. A Nhe I map of the Deinococcus radiodurans genome (3.1 Mb) with a resolution of 29 kb was generated by both manual map assembly and computational means. A second chromosome (415 kb) was also discovered using Optical Mapping. A new approach was developed to anchor any sequences to optical maps aided by Southern blotting analysis. A preliminary Xba I map, with an average restriction fragment size of 17.7 kb, was constructed for the unicellular parasite, Trypanosoma brucei, which causes sleeping sickness in humans. The contigs consisted of 10 maps and spanned 12.6 Mb. A composite map was aligned with a sequenced BAC clone derived from chromosome II. One region containing putative Ingi repeats was also detected. Whole genome optical maps have significantly facilitated the sequence assembly, gap closure and verification efforts for the large-scale sequencing of Deinococcus radiodurans and Trypanosoma brucei genomes. A pilot project for construction of a high-resolution reference map of the entire human genome was carried out. Preliminary data showed good correspondence with a statistical model of map construction developed by Lander and Waterman. In order to achieve the goal of high throughput and high reliability for Optical Mapping, a system using a cross-linked acrylamide overlay was developed. We have defined conditions that reproducibly resulted in excellent digestion efficiencies of 75%--80% and the routine retention of restriction fragments as small as 1 kb. Patterns of a 4-cutter enzyme, HinP1 I, were also evaluated using the overlay system. The preliminary results will provide the foundation for the development of an ultra-high resolution Optical Mapping system.

ISBN: 0599472979Subjects--Topical Terms:

1017730
Biology, Genetics.

Whole-genome shotgun optical mapping of the Deinococcus radiodurans and Trypanosoma brucei genomes; and the development of a new surface system for optical mapping.
LDR:02985nmm 2200289 4500 001 1862006
005 20041215074547.5
008 130614s1999 eng d
020 $a 0599472979
035 $a (UnM)AAI9945317
035 $a AAI9945317
040 $a UnM $c UnM
100 1 $a Qi, Rong. $3 1941777
245 1 0 $a Whole-genome shotgun optical mapping of the Deinococcus radiodurans and Trypanosoma brucei genomes; and the development of a new surface system for optical mapping.
300 $a 200 p.
500 $a Source: Dissertation Abstracts International, Volume: 60-09, Section: B, page: 4390.
500 $a Adviser: David C. Schwartz.
502 $a Thesis (Ph.D.)--New York University, 1999.
520 $a Optical Mapping is a proven means for the rapid construction of accurate, ordered restriction maps from ensembles of individual surface-mounted DNA molecules. Whole genome shotgun Optical Mapping was used to analyze the genome of Deinococcus radiodurans, Trypanosoma brucei and Homo sapiens. A Nhe I map of the Deinococcus radiodurans genome (3.1 Mb) with a resolution of 29 kb was generated by both manual map assembly and computational means. A second chromosome (415 kb) was also discovered using Optical Mapping. A new approach was developed to anchor any sequences to optical maps aided by Southern blotting analysis. A preliminary Xba I map, with an average restriction fragment size of 17.7 kb, was constructed for the unicellular parasite, Trypanosoma brucei, which causes sleeping sickness in humans. The contigs consisted of 10 maps and spanned 12.6 Mb. A composite map was aligned with a sequenced BAC clone derived from chromosome II. One region containing putative Ingi repeats was also detected. Whole genome optical maps have significantly facilitated the sequence assembly, gap closure and verification efforts for the large-scale sequencing of Deinococcus radiodurans and Trypanosoma brucei genomes. A pilot project for construction of a high-resolution reference map of the entire human genome was carried out. Preliminary data showed good correspondence with a statistical model of map construction developed by Lander and Waterman. In order to achieve the goal of high throughput and high reliability for Optical Mapping, a system using a cross-linked acrylamide overlay was developed. We have defined conditions that reproducibly resulted in excellent digestion efficiencies of 75%--80% and the routine retention of restriction fragments as small as 1 kb. Patterns of a 4-cutter enzyme, HinP1 I, were also evaluated using the overlay system. The preliminary results will provide the foundation for the development of an ultra-high resolution Optical Mapping system.
590 $a School code: 0146.
650 4 $a Biology, Genetics. $3 1017730
650 4 $a Biology, Microbiology. $3 1017734
650 4 $a Biophysics, General. $3 1019105
690 $a 0369
690 $a 0410
690 $a 0786
710 2 0 $a New York University. $3 515735
773 0 $t Dissertation Abstracts International $g 60-09B.
790 1 0 $a Schwartz, David C., $e advisor
790 $a 0146
791 $a Ph.D.
792 $a 1999
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9945317