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Messenger RNA nuclear export.

Lei, Elissa Philyn.

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Messenger RNA nuclear export.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Messenger RNA nuclear export./
作者:	Lei, Elissa Philyn.
面頁冊數:	136 p.
附註:	Source: Dissertation Abstracts International, Volume: 64-01, Section: B, page: 0030.
Contained By:	Dissertation Abstracts International64-01B.
標題:	Biology, Cell. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3076904
ISBN:	0493975764

Messenger RNA nuclear export.
Lei, Elissa Philyn.

Messenger RNA nuclear export. - 136 p.

Source: Dissertation Abstracts International, Volume: 64-01, Section: B, page: 0030.

Thesis (Ph.D.)--Harvard University, 2003.

In eukaryotic cells, the nuclear export of mRNA is essential for gene expression. The S. cerevisiae hnRNP protein Npl3 shuttles between the nucleus and the cytoplasm and is required for mRNA export. A screen to identify genes necessary for Npl3 export identified a mutation in SPT15, which encodes TATA-binding protein (T13P). Furthermore, NPL3 interacts genetically and physically with the basal transcription machinery. Assayed by chromatin immunoprecipitation, Npl3 and another key mRNA export factor, Yra1, are recruited to RNA polymerase II (Pol II) transcribed genes dependent on ongoing transcription. These results suggest a model in which transcription promotes the efficiency of mRNA export by stimulating the recruitment of mRNA export factors to the site of transcription.

ISBN: 0493975764Subjects--Topical Terms:

1017686
Biology, Cell.

Messenger RNA nuclear export.
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500 $a Source: Dissertation Abstracts International, Volume: 64-01, Section: B, page: 0030.
500 $a Adviser: Pamela A. Silver.
502 $a Thesis (Ph.D.)--Harvard University, 2003.
520 $a In eukaryotic cells, the nuclear export of mRNA is essential for gene expression. The S. cerevisiae hnRNP protein Npl3 shuttles between the nucleus and the cytoplasm and is required for mRNA export. A screen to identify genes necessary for Npl3 export identified a mutation in SPT15, which encodes TATA-binding protein (T13P). Furthermore, NPL3 interacts genetically and physically with the basal transcription machinery. Assayed by chromatin immunoprecipitation, Npl3 and another key mRNA export factor, Yra1, are recruited to RNA polymerase II (Pol II) transcribed genes dependent on ongoing transcription. These results suggest a model in which transcription promotes the efficiency of mRNA export by stimulating the recruitment of mRNA export factors to the site of transcription.
520 $a In order to determine what mechanisms control the cotranscriptional recruitment of mRNA export factors, the effect of cis- and trans -acting factors were examined. Npl3 is recruited exclusively to Pol II transcribed genes. When a gene normally synthesized by Pol I is produced by Pol II, Npl3 is nevertheless recruited indicating that Pol II transcription is sufficient for Npl3 cotranscriptional recruitment independent of RNA sequence. However, Pol II transcription is not sufficient to recruit Yra1 to a transcribing gene. In contrast, Yra1 associates preferentially with the intron of genes in a splicing dependent manner. Additionally, 3' end formation is required for Yra1 recruitment to all genes. These results suggest that cotranscriptional pre-mRNA processing stimulates the recruitment of export factors.
520 $a The Npl3 export screen also identified a gene known to be involved in mRNA export, MTR2. Using the mtr2 - mutant as a starting point, a genetic screen was performed and identified SAC3, which encodes an NPC associated protein. Mutation of SAC3 causes nuclear accumulation of mRNA and lethality in combination with mutations in other genes required for mRNA export. Furthermore, Sac3 interacts physically with multiple mRNA export factors. Using immunoelectron microscopy, Sac3 was found to localize predominantly to the cytoplasmic fibrils of the NPC. Finally, mutation of SAC3 mislocalizes Mex67, an mRNA transport receptor, and these results suggest that SAC3 functions at a terminal stage of RNP translocation through the NPC.
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790 1 0 $a Silver, Pamela A., $e advisor
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791 $a Ph.D.
792 $a 2003
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